Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Antioxidative Effects of Doenjang Fermented Using Bacillus subtilis DJI

Bacillus subtilis DJI을 이용하여 제조한 된장의 항산화효과

  • Lee, Jae-Joon (Department of Food and Nutrition, Chosun University) ;
  • Lee, Yu-Mi (Department of Food and Nutrition, Chosun University) ;
  • Chang, Hae-Choon (Department of Food and Nutrition, Chosun University) ;
  • Lee, Myung-Yul (Department of Food and Nutrition, Chosun University)
  • 이재준 (조선대학교 식품영양학과) ;
  • 이유미 (조선대학교 식품영양학과) ;
  • 장해춘 (조선대학교 식품영양학과) ;
  • 이명렬 (조선대학교 식품영양학과)
  • Published : 2009.08.30
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Abstract

We investigated the antioxidative effects of solvent extracts of doenjang fermented using Bacillus subtilis DJI (DJI doenjang) in vitro. The solvents used for extraction were ethanol, n-hexane, and water. The antioxidative activities of DJI doenjang solvent extracts were measured by estimation of peroxide value, the presence of linoleic acid level, and nitrite scavenging activity, the Rancimat test, and 1,1-diphenyl-2-picryl hydrazyl (DPPH) free-radical generation, in comparison with the commercial antioxidant butylated hydroxytoluene (BHT). The peroxide value of an ethanol extract was lower than those obtained using n-hexane and water extracts. Furthermore, the peroxide value of the ethanol extract was similar to that obtained after BHT treatment. The nitrite scavenging activity was 23.36% after addition of 600 ppm DJI doenjang ethanol extract, and the DPPH free-radical scavenging activity was 19.06% under same condition, which shows that DJI doenjang ethanol extract exhibited lower antioxidative capacities than did BHT. In the Rancimat test, the ethanol extract (11.20 min induction time), n-hexane extract (7.58 min induction time), and water extract (8.26 min induction time) after treatment with 600 ppm DJI doenjang extracts demonstrated longer induction periods than did BHT (6.94 min). These results indicate that DJI doenjang has potential anti-oxidative activity.

B. subtilis DJI 균주로 접종 후 천일염을 첨가하여 제조한 B. subtilis DJI 된장의 에탄올, 물 및 헥산의 용매별 항산화효과를 비교하였다. B. subtilis DJI 된장의 에탄올 추출물 수율은 15.9%, 헥산 추출물 수율은 14.9% 및 물 추출물 수율은 26.9% 범위로 물 추출물 수율이 가장 높았다. 과산화물가는 B. subtilis DJI 된장 용매별 추출물 중에서 에탄올 추출물이 300 ppm에서 600 ppm으로 농도를 증가하였을 경우 저하되어 과산화물 생성 억제효과가 있었으며, 합성항산화제인 BHT와 유사한 경향을 보였다. 아질산염 소거능은 B. subtilis DJI 된장 에탄올 추출물이 300 ppm과 600 ppm 농도에서 각각 18.26%와 23.36%로 가장 높았고, 물 추출물에서 10.23%와 12.46%였고, 헥산 추출물에서는 2.62%와 5.51%로 가장 낮아 헥산 추출물의 경우 항산화효과가 아주 미비한 것으로 나타났다. 전자공여능도 아질산염 소거능과 비슷한 경향을 보여 시료 농도가 증가할수록 항산화효과가 증가하였고, 에탄올 추출물이 가장 우수한 항산화효과를 나타내었다. 그러나 B. subtilis DJI 된장 추출물의 아질산염 소거능과 전자공여능은 합성항산화제인 BHT에 비해서는 낮은 경향이었다. Rancimat 방법으로 측정한 항산화효과도 두 농도 모두 B. subtilis DJI 된장 에탄올 추출물의 항산화효과가 가장 우수하였으나, 물과 헥산 추출물은 항산화효과에 영향을 미치지 않았다.

Keywords

References

  1. Korea Ministry of Health and Welfare. (2003) In: Depth analysis on 2001 national health and nutrition surveynutrition survey. KHIDI, Seoul, Korea. p 188-200
  2. Lee, J.H. and Min, D.B. (2006) Nutraceuticals, aging, and food oxidation. Handbook of Functional Lipids. Taylor & Francis Group, LLC, CRC Press, USA. p 325-350
  3. Shin, M.R. and Joo, K.j. (1999) Fractionated volatile flavor components of soybean paste by dynamic headspace method. Korean j. Soc. Food Sci. Nutr., 28, 305-311
  4. Shin, M.R. and Joo, K.J. (1999) Fractionated volatile flavor components of soybean paste by dynamic headspace method. Korean J. Soc. Food Sci. Nutr., 28, 305-311
  5. Enrique Cadenrs, M.D. and Lester, P. (2002) Handbook of antioxidants. MarcelDekker, New York, p.317-318
  6. Messina, M. (1995) Modern application for an ancient mean, soybeans and the prevention and treatment of chronic disease. J. Nutr., 125, 567-572
  7. Park, K.Y, Moon, S.H., Cheigh, H.S. and Baik, H.S. (1996) Antimutagenic effect of Doenjang (Korean soy paste). J. Food Sci. Nutr., 1, 151-156
  8. Kurechi, T., Kikuda, S. and Hasunuma, M. (1981) Inhibition of N-nitrosamine formation by soya products. Food Cosmet. Toxicol., 19, 425-450 https://doi.org/10.1016/0015-6264(81)90445-4
  9. Han, E.S., Lee, H.J. and Shon, D.W. (1993) Effect of surface hydrophobicity of soybean peptides on the concentration of serum cholesterol and fecal steroid excretion in rats. Korean J. Food Sci. Technol., 25, 571-575
  10. Yu, R.N., Chung, D.K., Nam, H.S. and Shin, Z.I. (1996) Effect of soybean hydrolysate on hypertension on spontaneous ely hypertensive rats. J. Korean Soc. Food Sci. Nutr., 25, 1031-1036
  11. Rhee, C.H., Kim, B.S., Shin, M.K., Woo, C.J., Kim, J.H., Kwon, K.Y. and Park, H.D. (2008) Changes in enzyme activity and physiological flUlctionality of deonjang (soybean paste) prepared with extracts of Phenillinus linteus. Korean J. Food Preserv., 15, 736-742
  12. Lee, K.I., Moon, R.J., Lee, S.J. and Park, K.Y. (2001) The quality assessment of doenjang with Japanese apricot, garlic and ginger, and samjang. Korean J. Soc. Food Cookery Sci., 17, 472-477
  13. Park, W.P., Kim, N.D., Lee, S.C., Kim, S.Y and Cho, S.H. (2006) Effects of powder and concentrates of Prnus mume on the quality of doenjang during fermentation. Korean J. Food Preserv., 13, 574-580
  14. Lee, J.S., Kwon, S.J., Ahn, C. and Yoo, J.Y (1997) Enzyme activities and physiological functionality of yeasts from traditional Meju. Kor. J. Appl. Biotechnol., 25, 448-453
  15. Chang, M. and Chang, H.C (2007) Characteristics of bacterial-koji and doenjang (soybean paste) made by using Bacillus subtilis DJI. Kor. J. Microbiol. Biotechnol., 35, 325-333
  16. Yoo, SK, Kang, S.M. and Noh, YS. (2000) Quality properties on soy bean pastes made with microorganism isolation from traditional soy bean pastes. Korean J. Food Sci. Technol., 32, 1266-1270
  17. Shon, M.Y, Seo, K.I., Park, S.K., Cho, Y.S. and sung, N.J. (2001) Some biological activities and isoflavone content of chunglatkjang prepared with black beans and Bacillus strains. J. Korean Soc. Food Sci. Nutr., 30, 662-667
  18. Shon, M.Y. (1999) Physiochemical properties and biological activities of chunglatkjang produced from Korean black bean. Ph.D. Dissertation. Gyeongsang National University. p.37-50
  19. Back, L.M., Park, L.Y, Park, K.S. and Lee, S.H. (2008) Effect of starter cultures on the fermentative characteristics of chunglatkjang. Korean J. Food Sci. Technol., 40, 400-405
  20. Song, J.Y, Ahn, C.W. and Kim, J.K. (1984) Flavor components produced by microorganism during fermentation of Korean ordinary soybean paste. Kor. J. Appl. Microbiol. Bioeng., 12, 147-152
  21. A.O.A.C. (1995) Official Methods of Analysis. 16th eds., Association of Official Analytical Chemists, Washington, D.C, USA
  22. Kim, H.K., Kim, Y.E., Do, J.R. and Lee, B.Y (1995) Antioxidative activity and physiological activity of some Korean medicinal plants. Korean J. Food Sci. Technol., 27, 80-89
  23. Hayase, F. and Kato, H. (1984) Antioxidative components of sweet potatoes. J. Nutr. Sci. Vitamin, 30, 37-42 https://doi.org/10.3177/jnsv.30.37
  24. Kato, H., Lee, I.E., Cheyen. N,Y., Kim, S.B. and Hayase, F. (1987) fuhibitcry of nit:J:arunine fonrntion by nondialyzable melanoidins. Agric. Biol. Chem., 5, 1333-1335
  25. Joo, K.J. and Kim, J.J. (2002) Oxidative stability and flavor compolUlds of sesame oils blended with vegetable oils. Korean J. Food Sci. Technol., 34, 499-502
  26. Blois, M.S. (1958) Antioxidant determination by the use of a stable free radical. Nature, 26, 1199-1200
  27. Kwon, S.H., Lee, K.B., Im, K.S., Kim, S.O. and Park, K.Y. (2006) Weight reduction and lipid lowering effects of Korean traditional soybean fermented products. J. Korean Soc. Food Sci. Nutr., 35, 1194-1199 https://doi.org/10.3746/jkfn.2006.35.9.1194
  28. Lee, S.L. and Kim, J.G. (2005) Inhibitory effects of doenjang (Korean fermented soybean paste) and soybean extracts on the growth of KB cells. Kor. J. Envir. Health, 31, 444-450
  29. Oh, H.J. and Kim, C.S. (2007) Antioxidant and nitrite scavenging ability of fermented soybean foods (Chunglatkjang, Doenjang). J. Korean Soc. Food Sci. Nutr., 36, 1503-1510 https://doi.org/10.3746/jkfn.2007.36.12.1503
  30. Park, K.Y. and Jung, K.O. (2005) Fermented soybean products as functional foods: functional properties of Deonjang (fermented soybean paste). In Asian Functional Foods. Taylor & Francis Group, LLC, CRC Press, USA. p.555-596
  31. Kwon, S.H. and Shon, M.Y (2004) Antioxidant and anticarcinogenic effects of traditional doenjang during maturation periods. Korean J. Food Preserv., 11, 461-467
  32. Bae, E.A. and Moon, G.S. (1997) A study on the antioxidative of Korean soybeans. J. Korean Food Sci. Nutr., 26, 203-208
  33. Choe, G.S., Lim, S.Y and Choi, J.S. (1998) Antioxidant and nitrite scavenging effect of soybean, meju and doenjang. Korean J. Life Sci., 8, 473-478
  34. Archer, M.C. and Weisman, M. (1975) Reaction of nitrite with ascorbate and its relation to nitrosamine formation. J. Natl. Cancer lust., 54, 1203-1205
  35. Kim, J.S. and Yoon, S. (1999) Isoflavone content and $\beta$-glucosidase activity of the soybeans, meju, and deonjang. Korean J. Food Sci. Technol., 31, 1405-1409
  36. Cooney, R.V. and Ross, P.D. (1989) N-nitrosation and N-nitration of morpholine by nitrogen dioxide: Inhibition by ascorbate, glutathione and a-tocopherol. J. Agric. Food Chem., 35, 83-90
  37. Gray, J.I. and Dugan, J.R. (1975) Inhibition of n-nitrosamine formation in model food systems. J. Food Sci., 40, 981-984 https://doi.org/10.1111/j.1365-2621.1975.tb02248.x
  38. Kim, A.H., Lee, J.J. and Lee, M.Y. (2009) Antioxidant effect of Angelica acutiloba kitagawa ethanol extract. J. Life Sci., 19, 117-122 https://doi.org/10.5352/JLS.2009.19.1.117
  39. Cha, G.S. and Choi, C.U. (1990) Determination of oxidation stability of perilla oil by the Rancimat method. Korean J. Food Sci. Technol., 22, 61-65
  40. Kim, B.S., Rhee, C.H., Hong, Y.A., Woo, C.J., Jang, C.M., Kim, Y.B. and Park, H.D. (2007) Changes of enzyme activity and physiological functionality of traditional doenjang during fermentation using Bacillus sp. SP-KSW3. Korean J. Food Preserv., 14, 545-551
  41. Rhee, C.H., Kim, W.C., Rhee, I.K. and Park, H.D. (2008) Effect of inoculation of Bacillus subtilis cells on the fermentation of Korean traditional soy paste (Deonjang). Korean J. Food Preserv., 15, 598-605