Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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PROLIFERATION OF ENDOTHELIAL PROGENITOR CELLS BY OSTEOGENIC DIFFERENTIATION OF PERIOSTEAL-DERIVED CELLS

골막기원세포의 조골세포 분화과정에서 나타나는 혈관내피전구세포의 증식

  • Kim, Jong-Ryoul (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Song, Jung-Ho (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Kim, Uk-Kyu (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Park, Bong-Wook (Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine and Institute of Health Sciences, Biomedical Center (BK21)) ;
  • Hah, Young-Sool (Clinical Research Institute, Gyeongsang National University Hospital) ;
  • Kim, Jin-Hyun (Clinical Research Institute, Gyeongsang National University Hospital) ;
  • Kim, Deok Ryong (Department of Biochemistry, Gyeongsang National University School of Medicine and Institute of Health Sciences, Biomedical Center (BK21)) ;
  • Cho, Yeong-Cheol (Department of Oral and Maxillofacial Surgery, College of Medicine, Ulsan University) ;
  • Sung, Iel-Yong (Department of Oral and Maxillofacial Surgery, College of Medicine, Ulsan University) ;
  • Byun, June-Ho (Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine and Institute of Health Sciences, Biomedical Center (BK21))
  • 김종렬 (부산대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 송정호 (부산대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 김욱규 (부산대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 박봉욱 (경상대학교 의학전문대학원 구강악안면외과학교실, 경상대학교 건강과학연구원, 의생명과학사업단 (BK21)) ;
  • 하영술 (경상대학교병원 임상의학연구소) ;
  • 김진현 (경상대학교병원 임상의학연구소) ;
  • 김덕룡 (경상대학교 의학전문대학원 생화학교실, 경상대학교 건강과학연구원, 의생명과학사업단 (BK21)) ;
  • 조영철 (울산대학교 의과대학 구강악안면외과학교실) ;
  • 성일용 (울산대학교 의과대학 구강악안면외과학교실) ;
  • 변준호 (경상대학교 의학전문대학원 구강악안면외과학교실, 경상대학교 건강과학연구원, 의생명과학사업단 (BK21))
  • Published : 2009.08.31
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Abstract

Purpose : The purpose of this study was to examine the expression of various angiogenic factors during osteoblastic differentiation of periostealderived cells and the effects of osteogenic inductive medium of periosteal-derived cells on the proliferation of endothelial progenitor cells. Materials and methods : Periosteal-derived cells were obtained from mandibular periosteums and introduced into the cell culture. After passage 3, the cells were divided into two groups and cultured for 21 days. In one group, the cells were cultured in the DMEM supplemented with osteogenic inductive agent, including 50g/ml L-ascorbic acid 2-phosphate, 10 nM dexamethasone and 10 mM -glycerophosphate. In the other group, they were cultured in DMEM supplemented without osteogenic inductive agent. VEGF isoforms, VEGFR-1, VEGFR-2, and neuropilin-1 mRNA expression was observed. Human umbilical cord blood-derived endothelial progenitor cell proliferation was also observed. Results : The expression of VEGF isoforms was higher in osteogenic inductive medium than in non-osteogenic inductive medium. The expression of VEGFR-2 was also higher in osteogenic inductive medium than in non-osteogenic inductive medium. However, the expression of VEGFR-1 and neuropilin-1 was similar in both osteogenic inductive medium and non-osteogenic inductive medium. In addition, conditioned medium from differentiated periosteal-derived cells stimulated human umbilical cord blood-derived endothelial progenitor cell numbers compared to conditioned medium from non-differentiated periosteal-derived cells. Conclusion : These results suggest that in vitro osteoblastic differentiation of periosteal-derived cells has angiogenic capacity to support endothelial progenitor cell numbers.

Keywords

References

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