Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antiinflammatory Effect of Aqueous Extract from Red Pepper on Lipopolysaccharide Induced Inflammatory Responses in Murine Macrophages

홍고추가루 수용성 추출물의 항염증 효과

  • Kwon, Hyuck-Se (Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University) ;
  • Shin, Hyun-Kyung (Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University) ;
  • Kwon, Sang-O (S&D Co., Ltd) ;
  • Yeo, Kyung-Mok (S&D Co., Ltd) ;
  • Kim, Sang-Moo (S&D Co., Ltd) ;
  • Kim, Bok-Nam (Dept. of Tourism and Food Service Cuisine, Hallym College) ;
  • Kim, Jin-Kyung (Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University)
  • 권혁세 (한림대학교 식의약품의 효능평가 및 기능성 소재개발센터) ;
  • 신현경 (한림대학교 식의약품의 효능평가 및 기능성 소재개발센터) ;
  • 권상오 ((주)에스엔디) ;
  • 여경목 ((주)에스엔디) ;
  • 김상무 ((주)에스엔디) ;
  • 김복남 (한림성심대학 관광외식조리과) ;
  • 김진경 (한림대학교 식의약품의 효능평가 및 기능성 소재개발센터)
  • Published : 2009.10.31
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Abstract

Inflammation is a pivotal component of a variety of diseases, such as atherosclerosis and tumour progression. Various naturally occurring phytochemicals exhibit antiinflammatory activity and are considered to be potential drug candidates against inflammation-related pathological processes. Red pepper is the most consumed species in Korea. However, the antiinflammatory effects of red pepper have not been characterized. Thus, the present study was designed to evaluate the effects of the aqueous extract from red pepper (RPAE) on lipopolysaccharide (LPS)-induced inflammatory responses in murine macrophages. RPAE demonstrated strong antiinflammatory activity through its ability to reduce nitric oxide and prostaglandin $E_2$ production in the LPS-stimulated mouse macrophage cell, RAW264.7. It also inhibited the production of interleukin-6 (IL-6) on the LPS-stimulated RAW264.7 cells. Further study indicated that LPS-stimulated induction of inducible nitric oxide synthase and cyclooxygenase-2 was significantly inhibited by RPAE exposure (1,000 mg/mL) in RAW264.7 cells. Collectively, these data suggest that the use of RPAE may be a useful therapeutic approach to various inflammatory diseases.

본 연구의 목적은 향신료로서 한국인의 식생활에 중요한 위치를 차지하고 있는 홍고추가루 수용성 추출물의 항염증 활성을 검토해보고자 수행되었다. 그 결과 홍고추가루의 수용성 추출물은 LPS 처리에 의한 NO 및 $PGE_2$의 생성을 현저히 억제시키는 것을 관찰할 수 있었으며, NO의 생합성효소인 iNOS 단백질의 발현 또한 억제시킴을 확인할 수 있었다. 홍고추가루의 수용성 추출물은 염증성 사이토카인인 IL-6의 생성억제 효과도 탁월하였다. 이러한 연구결과로 볼 때 고추의 수용성 추출물이 대식세포에 의해 생성되는 염증반응의 매개물질인 NO, $PGE_2$, IL-6의 생성을 억제함으로 염증반응을 완화시켜 주는 것으로 판단된다. 차후 항염증 활성을 갖는 홍고추가루의 수용성 추출물의 성분을 동정하는 연구가 필요할 것으로 사료되며, 고추가 식품유래의 항염증활성을 갖는 제품 개발에 있어 유용한 식품자원 원료로 사용 될 수 있을 것으로 생각된다.

Keywords

References

  1. Daood HG, Vinkler M, Markus F, Hebshi EA, Biacs PA. 1996. Antioxidant vitamin content of spice red pepper (paprika) as affected by technological and varietal factors. Food Chem 55: 365-372. https://doi.org/10.1016/0308-8146(95)00136-0
  2. Krinsky NI. 1994. The biological properties of carotenoids. Pure Appl Chem 66: 1003-1010. https://doi.org/10.1351/pac199466051003
  3. Krinsky NI. 2001. Carotenoids as antioxidants. Nutrition 17: 815-817. https://doi.org/10.1016/S0899-9007(01)00651-7
  4. Matucci CM, McCarthy G, Lombardi A, Pignone A, Partsch G. 1995. Neurogenic influences in arthritis: potential modification by capsaicin. J Rheumatol 22: 1447-1449.
  5. Diepvens K, Westerterp KR, Westerterp-Plantenga MS. 2007. Obesity and thermogenesis related to the consumption of caffeine, ephedrine, capsaicin, and green tea. Am J Physiol Regul Integr Comp Physiol 292: R77-85. https://doi.org/10.1152/ajpregu.00832.2005
  6. Sicuteri F, Fusco BM, Marabini S, Campagnolo V, Maggi CA, Geppetti P, Fanciullacci M. 1989. Beneficial effect of capsaicin application to the nasal mucosa in cluster headache. Clin J Pain 5: 49-53. https://doi.org/10.1097/00002508-198903000-00010
  7. Watson CP, Evans RJ, Watt VR. 1988. Post-herpetic neuralgia and topical capsaicin. Pain 33: 333-340. https://doi.org/10.1016/0304-3959(88)90292-8
  8. Kang SN, Chung SW, Kim TS. 2001. Capsaicin potentiates 1,25-dihydoxyvitamin $D_3-$ and ATRA-induced differentiation of human promyelocytic leukemia HL-60 cells. Eur J Pharmacol 420: 83-90. https://doi.org/10.1016/S0014-2999(01)00994-3
  9. Zhang J, Nagasaki M, Tanaka Y, Morikawa S. 2003. Capsaicin inhibits growth of adult T-cell leukemia cells. Leuk Res 27: 275-283. https://doi.org/10.1016/S0145-2126(02)00164-9
  10. Palevitch D, Craker LE. 1995. Nutritional and medicinal importance of red pepper (Capsicum spp.). J Herbs Spices Med Plants 3: 55-83.
  11. Ito K, Nakazato T, Yamato K, Miyakawa Y, Yamada T, Hozumi N, Segawa K, Ikeda Y, Kizaki M. 2004. Induction of apoptosis in leukemic cells by homovanillic acid derivative, capsaicin, through oxidative stress: Implication of phosphorylation of p53 at ser-15 residue by reactive oxygen species. Cancer Res 64: 1071-1078. https://doi.org/10.1158/0008-5472.CAN-03-1670
  12. Kim JD, Kim JM, Pyo JO, Kim SY, Kim BS, Yu R, Han IS. 1997. Capsaicin can alter the expression of tumor forming- related gene which might be followed by induction of apoptosis of a Korean stomach cancer cell line, SNU-1. Cancer Lett 120: 235-241. https://doi.org/10.1016/S0304-3835(97)00321-2
  13. Mori A, Lehmann S, O'Kelly J, Kumagai T, Desmond JC, Pervan M, McBride WH, Kizaki M, Koeffler HP. 2006. Capsaicin, a component of red peppers, inhibits the growth of androgen-independent, p53 mutant prostate cancer cells. Cancer Res 66: 3222-3229. https://doi.org/10.1158/0008-5472.CAN-05-0087
  14. Tizard IR. 1986. Immunology: an introduction inflammation. 2nd ed. Saunders College Pubulishing, New York, NY, USA. p 423-441.
  15. Lawrence T, Wiilloughby DA, Gilroy DW. 2002. Anti-inflammatory lipid mediators and insights into the resolution of inflammation. Nat Rev Immunol 2: 787-795. https://doi.org/10.1038/nri915
  16. Higuchi M, Hisgahi N, Taki H, Osawa T. 1990. Cytolytic mechanisms of activated macrophages. Tumor necrosis factor and L-arginine-dependent mechanisms act synergistically as the major cytolytic mechanisms of activated macrophages. J Immunol 144: 1425-1431.
  17. Laflamme N, Rivest S. 2001. Toll-like receptor 4: The missing link of the cerebral innate immune response triggered by circulating gram-negative bacterial cell wall components. FASEB J 15: 155-163. https://doi.org/10.1096/fj.00-0339com
  18. Willeaume V, Kruys V, Mijatovic T, Huez G. 1996. Tumor necrosis factor-alpha production induced by viruses and by lipopolysaccharide in macrophages: similarities and differences. J Inflamm 46: 1-12.
  19. Iontcheva I, Amar S, Zawawi KH, Kantarci A, Van Dyke TE. 2004. Role for moesin in lipopolysaccharide-stimulated signal transduction. Infect Immun 72: 2312-2320. https://doi.org/10.1128/IAI.72.4.2312-2320.2004
  20. Baud V, Karin M. 2001. Signal transduction by tumor necrosis factor and its relatives. Trend Cell Biol 11: 372-377. https://doi.org/10.1016/S0962-8924(01)02064-5
  21. Park JY, Kawada T, Han IS, Kim BS, Goto T, Takahashi N, Fushiki T, Kurata T, Yu R. 2004. Capsaicin inhibits the production of tumor necrosis factor alpha by LPS-stimulated murine macrophages, RAW 264.7: a PPARgamma ligand- like action as a novel mechanism. FEBS Lett 572: 266-270. https://doi.org/10.1016/j.febslet.2004.06.084
  22. Kim CS, Kawada T, Kim BS, Han IS, Choe SY, Kurata T, Yu R. 2003. Capsaicin exhibits anti-inflammatory property by inhibiting IkB-a degradation in LPS-stimulated peritoneal macrophages. Cell Signal 15: 299-306. https://doi.org/10.1016/S0898-6568(02)00086-4
  23. Oboh G, Rocha JB. 2008. Hot pepper (Capsicum spp.) protects brain from sodium nitroprusside- and quinolinic acid-induced oxidative stress in vitro. J Med Food 11: 349-355. https://doi.org/10.1089/jmf.2007.341

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