Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Antimicrobial Susceptibility of Escherichia coli Isolated from Fish Farms on the Southern Coast of Korea

남해안 어류양식장에서 분리된 Escherichia coli에 대한 항균제 감수성

  • 손광태 (국립수산과학원 남해수산연구소 증식과) ;
  • 오은경 (국립수산과학원 식품안전연구단) ;
  • 박큰바위 (국립수산과학원 남해수산연구소 증식과) ;
  • 권지영 (국립수산과학원 남해수산연구소 증식과) ;
  • 이희정 (국립수산과학원 식품안전연구단) ;
  • 이태식 (국립수산과학원 양식환경연구소) ;
  • 김지회 (국립수산과학원 양식환경연구소)
  • Published : 2009.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Three-hundred and sixteen Escherichia coli strains from seawater, and a variety of farmed fishes, including oliver flounder (Paralichthys olivaceus), black rock fish (Sebastes schlegeli), red sea bream (Pagrus major) and sea bass (Lateolabrax japonicus) between May to October in 2004, were tested by agar dilution method to determine their susceptibility patterns to 17 antimicrobial agents. Overall, 92.1% of Escherichia coli isolates from samples showed antimicrobial resistance to at least one antimicrobial agent and the multiple resistance was seen in 173 isolates (54.7%). The resistance of E. coli isolates to tetracycline (74.1%) was highest, followed by cephalothin (69.9%), doxycycline (66.5%), streptomycin (47.2%), ampicillin (46.2%), cefazolin (31.6%), enrofloxacin (31.0%). norfloxacin (28.2%). The most frequent resistance pattern was TE-D-CF-CIP-ENO-NOR-AM-S-C-SXT-AmC-CZ (14.7%), followed by CF (6.2%), TE (5.1%), TE-CF (4.5%) in 177 isolates from fishes and TE-D-CF (7.2%) followed by TE-D-CF-S (5.8%), CF and TE-D-S (3.6%) in 139 isolates from seawater.

Keywords

References

  1. Acar, J,F. and F.W, Goldstein. 1991. Disk susceptibility test. In: Antibiotics in Laboratory Medicine, Lorian, V. Ed., Williams & Wilkins, Baltimore, 17-52
  2. Aaestrup, F.M., F. Bager, N.E. Jesen, M. Madsen, A. Meyling and H.C. Wegener. 1998. Surveillance of antimicrobial resistance in bacteria isolated from food animals to antimicrobial growth promoters and related therapeutic agents in Denmark. APMIS, 106, 606-622
  3. A.P.H.A. 1970, Recommended Procedures for the Examination of Sea Water and Shellfish. 4th ed., American Public Health Association, Washington, D,C., 105 pp
  4. Von Baum, H. and R. Marre. 2005, Antimicrobial resistance of Escherichia coli and therapeutic implications. Int. J. Medic. Microbiol., 295, 503- 511 https://doi.org/10.1016/j.ijmm.2005.07.002
  5. Beam, J.W. and B. Buckley. 2006. Community acquired methicillin resistant Staphylococcus aureus: Prevalence and risk factors. J. Athl. Train., 41, 337- 340
  6. Cho, J.K. J.S. Ha and K.S. Kim, 2006. Antimicrobial drug resistance of Escherichia coli isolated from cattle, swine and chicken, Kor.J. Vet. Publ. Hlth., 30, 9-18
  7. Chio, S.H., YJ. Lee, B.H. Kim, KS. Kim, C.K. Park, D.H. Bae, J.K. Cho, J,W, Kim, RH. Kim and M.S, Kang. 2006. Antibiotic resistance and genotypic characterization of Escherichia coli isolated from pig farm environment. J, Bacteriol. Virol., 36, 159-165 https://doi.org/10.4167/jbv.2006.36.3.159
  8. Fey, P,D., T.J, Safranek, M.E, Rupp, E.F. Dunne, E. Ribot, p,c. Iwen, P.A. Bradford, F.J. Angulo and S. Hinrichs. 2000. Ceftriaxone-resistant salmonella infection acquired by a child from cattle. N, Engl. J. Med., 27, 1242-1249 https://doi.org/10.1097/01.smj.0000220882.84566.9f
  9. KFDA (Korean Food and Drug Administration), 2007. Monitoring of antimicrobial resistance on the food animals and meats. Seoul, 1-110
  10. Frieden, T.R., S.S. Munsiff, D.E. Low, B.M. Willey, G, Williams, Y. Faur, W. Eisner, S. Warren and B. Kreiswirth. 1993. Emergence of vancomycin resistant enterococci in New York City, Lancet, 342, 76-79 https://doi.org/10.1016/0140-6736(93)91285-T
  11. Gaskins, H.R., C.T. Collier and D,B. Anderson. 2002. Antibiotics as growth promotants: mode of action. Anim. Biothechnol., 13, 29-42 https://doi.org/10.1081/ABIO-120005768
  12. Heo, J.H, M.H. Jung, M.H. Cho, G.H. Kim, K.C. Lee, J.H. Kim and T.S, Jung. 2002, The study on fish disease with reference to bacterial susceptibility to antibiotics in the southern area of Kyeongnam. J. Vet. Clin., 19, 19-22
  13. Herwig, R.P., J.P. Gray and D.P. Weston. 1997. Antibacterial resistant bacteria in surficial sediments near salmon net-cage farms in Puget Sound, Washington, Aquaculture, 149, 263-283 https://doi.org/10.1016/S0044-8486(96)01455-X
  14. Kim, A.R., Y.M. Cho, S.K. Lim, M. Her, W.S. Jeong, S.C. Jung and J.H. Kown. 2007. Antimicrobial resistance of commensal bacteria isolated from food-producing animals. I. Antimicrobial resistance of Escherichia coli and Enterococcus spp, isolated from chicken faecal samples, Kor. J. Vet. Publ. Hlth., 31, 41-49
  15. Kruse, H. and H. Sorum. 1994. Transfer of multiple drug resistance plasmids between bacteria of diverse origins in natural microenvironemnts. Appl. & Environ. Microbiol., 60, 4015-4021
  16. Kummerer, K 2009, Antibiotics in the aquatic environment: a review-part I . Chemosphere, 417-434 https://doi.org/10.1016/j.chemosphere
  17. Kummerer, K and A. Henninger. 2003. Promoting resistance by the emission of antibiotics from hospitals and households into effluents, Clin, Microbiol. Infec., 9, 1203-1214 https://doi.org/10.1111/j.1469-0691.2003.00739.x
  18. Lau, M.M. and S.C. Ingham, 2001. Survival of faecal indicator bacteria in bovine manure incorporated into soil. Lett, Appl. Microbiol., 33, 131-136 https://doi.org/10.1046/j.1472-765x.2001.00962.x
  19. Lee, H., Y.H. Oh, S,G, Park and S,M. Choi. 2007. Antibiotic susceptibility and distribution of Vibrio parahaemolyticus isolated from the seafood, Kor. J. Env, Hlth., 33, 16-20
  20. Lee, J.I., G.Y. Han and H.H. Park. 2003. Characteristics and antibiotics susceptibility of Escherichia coli isolated from fIshery products. Korean J. Food & Nutr. 16, 111-115
  21. Levin, B.R., M. Lipsitch, V. Perrot, S. Schrag, R. Antia, L. Simonsen, N.M. Walker and F.M. Stewart. 1997. The population genetics of antibiotic resistance. Clin. Infect Dis., 24, Suppll, S9-16 https://doi.org/10.1093/clinids/24.Supplement_1.S9
  22. Lim, S.K., H.S. Lee, J.R. Byun, S.Y. Park and S.C. Jung. 2007a. Antimicrobial resistance of commensal bacteria isolated from food-producing animals. I. Antimicrobial resistance of Escherichia coli and Enterococcus spp. isolated from cattle faecal samples. Kor. J. Vet. Publ. Hlth., 31, 21- 29
  23. Lim, S.K., H.S. Lee, J.R. Byun, S.Y. Park and S.C. Jung. 2007b. Antimicrobial resistance of commensal bacteria isolated from food-producing animals. I. Antimicrobial resistance of Escherichia coli and EnteroCOCClls spp. isolated from pig faecal samples. Kor. J. Vet. Publ. Hlth., 31, 31- 39
  24. Longlois, B.E., K.A. Dawson, I. Leak and D.K Aaron. 1988. Antimicrobial resistance of fecal colifonns from pigs in a herd not exposed to antimicrobial agents for 126 months. Vet. Microbiol., 18, 147- 153
  25. Marano, N.N., S. Rossiter, K Stamey, K. Joyce, T.J. Barrett, L.K Tolldfson and F.J. Angulo. 2000. The national antimicrobial resistance monitoring system (NARMS) for enteric bacteria, 1996-1999: surveillance for action. J. Am. Vet. Med. Associ., 217, 1829-1830
  26. NCCLS. 2004. Performance standards for antimicrobial susccptibility tcsting. NCCLS document MlOO-S14. National Committee for Clinical Laboratory Standards, Wayne, PA
  27. Son, K.T., E.G. Oh, T.S. Lee, H.J. Lee, P.H. Kim and J.H. Kim. 2005. Antimicrobial susceptibility of Vibrio parahaemolyticus and Vibrio alginolyticus from fish farms on the southern coast of Korea. J. Kor. Fish. Soc., 38, 365-371
  28. Sung, M.S, J.H. Kim, J.S. Ha, J.K. Cho, S.Y. Seol and K.S. Kim. 2008. Biochemical properties and serotypes of pathogenic Escherichia coli isolated from poultry in Korca. Kor. J. Vet. Res., 48, 145- 151
  29. Tendencia, E.A. and L.D. Pena. 2001. Antibiotic resistance of bacteria from shrimp ponds. Aquaculture, 195, 193-204 https://doi.org/10.1016/S0044-8486(00)00570-6
  30. Tollefson, L., S.F. Altekruse and M.E. Potter. 1997. Therapeutic antibiotics in animal feeds and antibiotic resistance. Rev. Sci. Tech. 16, 709-715 https://doi.org/10.1128/AEM.67.4.1558-1564.2001
  31. Wise, R. 2002. Antimicrobial resistance: priorities for action. J. Antimicrob. Chemoth., 49, 585-586 https://doi.org/10.1093/jac/49.4.585
  32. You, J.Y., B.M. Moon, J.G. Oh, B.K Baek, L.G. Li, B.S. Kim, B.D. Stein and 1.R. Lee. 2006. Antimicrobial resistance of Escherichia coli 0157 trom cattle in Korea.. Int. J. Food Microbiol., 106, 74- 78 https://doi.org/10.1016/j.ijfoodmicro.2005.05.013

Cited by

  1. Antimicrobial Resistance in Escherichia coli Isolated from the Shellfish Farms in the Southern Coast of Korea vol.46, pp.5, 2013, https://doi.org/10.5657/KFAS.2013.0528
  2. Antimicrobial Resistance in Escherichia coli Isolated from Shellfish Farms on the West Coast of Korea vol.49, pp.1, 2016, https://doi.org/10.5657/KFAS.2016.0013
  3. 창선 해역의 지중해담치(Mytilus galloprovincialis) 양식장 및 육상오염원에서 분리한 대장균(Escherichia coli)의 항생제 내성 vol.49, pp.5, 2016, https://doi.org/10.5657/kfas.2016.0564
  4. 서해안 수산생물에서 분리한 대장균(Escherichia coli)의 항생제 내성 및 다제 내성 양상 비교 vol.54, pp.4, 2009, https://doi.org/10.5657/kfas.2021.0388