Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Hemicellulose Recovery from Rice Straw using Dilute Sulfuric Acid

묽은 황산을 사용하여 볏짚으로부터 헤미셀룰로오스 회수

  • Lee, Dong-Hun (Department of Chemical & Biological Engineering and ERI, Gyeongsang National University) ;
  • Kim, Chang-Joon (Department of Chemical & Biological Engineering and ERI, Gyeongsang National University) ;
  • Kim, Sung-Bae (Department of Chemical & Biological Engineering and ERI, Gyeongsang National University)
  • 이동헌 (경상대학교 공대 생명화학공학과 및 공학원) ;
  • 김창준 (경상대학교 공대 생명화학공학과 및 공학원) ;
  • 김성배 (경상대학교 공대 생명화학공학과 및 공학원)
  • Published : 2009.09.28
Download PDF
⟨ Previous Next ⟩

Abstract

Rice straw was pretreated using dilute sulfuric acid at reaction conditions covering two levels of reaction temperature (140, $150^{\circ}C$) and five levels of acid concentrations ($1.0{\sim}3.0%wt$). The production and decomposition rates of major components of rice straw indicating glucose, xylose, galactose and arabinose were investigated. The production rate of arabinose and the decomposition rate of xylose were greatest among them. The maximum attainable hemicellulose (xylose+galactose+arabinose) yield was about 80%. High acid concentration appears to favor the maximum yield but high temperature does not. The optimum condition was found to be $140^{\circ}C$, 2.5% and 20 minutes. The maximum glucose yields were almost same, around $16{\sim}18%$, regardless of reaction conditions.

희석 황산용액을 사용해 볏짚을 전처리했는데 반응조건은 2개의 반응온도(140, $150^{\circ}C$)와 5개의 황산농도($1.0{\sim}3.0 %wt$)를 사용했다. 볏짚을 구성하는 주요성분인 glucose, xylose, galactose와 arabinose의 생성과 분해속도를 조사했는데 생성속도는 arabinose, 분해속도는 xylose가 가장 컸다. 헤미셀룰로오스당 (xylose+galactose+arabinose)의 최고수율은 약 80%이었는데 최고수율에 도달하는데 산 농도가 높은 것은 유리하지만 반응온도가 높은 것은 불리하게 나타났다. 최적조건은 $140^{\circ}C$, 2.5%와 20분이었다. 최고 glucose 수율은 반응조건에 관계없이 거의 같았는데 대략 $16{\sim}18%$ 범위였다.

Keywords

References

  1. 소규호. 2009. 바이오에너지 마을 조성을 위한 제반조건 및 추진전략. Bioin 스페셜 zine. 11 호
  2. Bak, J. S., J. K. Ko, Y. H. Han, B. C. Lee, I.-G. Choi, and K. H. Kim. 2009. Improved enzymatic hydrolysis yield of rice straw using electron beam irradiation pretreatment, Bioresour. Technol. 100: 1285-1290 https://doi.org/10.1016/j.biortech.2008.09.010
  3. Dale, B. E., J. Weaver, and F. M. Byers. 1999. Extrusion processing for ammonia fiber explosion (AFEX). Appl. Biochem. Biotechnol. 77-79: 1-11 https://doi.org/10.1385/ABAB:77:1-3:35
  4. Fan, L. T., M. M. Gharpuray, and Y-H. Lee. 1987. Cellulose Hydrolysis. pp.5-20. Springer-Verlag, Berlin, Germany
  5. Karimi, K., S. Kheradmandina, and M. J. Taherzadeh. 2006. Conversion of rice straw to sugars by dilute-acid hydrolysis. Biomass Bioenergy. 30: 247-253 https://doi.org/10.1016/j.biombioe.2005.11.015
  6. Kim, B. J., Y. Y. Lee, and R. Torget. 1994. Modified percolation process in dilute-acid hydrolysis of biphasic hemicellulose. Appl. Biochem. Biotechnol. 45/46: 113-129 https://doi.org/10.1007/BF02941792
  7. Kim, S. B., D. M. Yum, and S. C. Park. 2000. Step-change variation of acid concentration in a percolation reactor for hydrolysis of hardwood hemicellulose. Appl. Biochem. Biotechnol. 72: 289-294 https://doi.org/10.1016/S0960-8524(99)00081-4
  8. Kim, S. B and J. W. Chun. 2004. Enhancement of enzymatic digestibility of recycled newspaper by addition of surfactant in ammonia-hydrogen peroxide treatment. Appl. Biochem. Biotechnol. 113-116: 1023-1031 https://doi.org/10.1385/ABAB:115:1-3:1023
  9. Kim, S. B., S. J. Kim, D. M. Yum, and S. C. Park. 1997. Kinetics in dilute acid hydrolysis of woody hemicellulose. Korean J. Biotechnol. Bioeng. 12(4): 402-409
  10. Kim, S. B. and Y. Y. Lee. 1996. Fractionation of herbaceous biomass by ammonia-hydrogen peroxide percolation treatment. Appl. Biochem. Biotechnol. 57/58: 147-156 https://doi.org/10.1007/BF02941695
  11. Kim, S. B. and Y. Y. Lee. 1987. Kinetics in acid catalyzed hydrolysis of hardwood hemicellulose. Biotechnol. Bioeng. Symp. No.17: 71-84
  12. Kuo, C.-H. and C.-K. Lee. 2009. Enhanced enzymatic hydrolysis of sugarcane bagasse by N-methylmorpholine-N-oxide pretreatment. Bioresour. Technol. 100: 866-871 https://doi.org/10.1016/j.biortech.2008.07.001
  13. Ma, H., W.-W. Liu, X. Chen, Y.-J. Wu, and Z.-L. Yu. 2009. Enhanced enzymatic saccharification of rice straw by microwave pretreatrnent. Bioresour. Technol. 100: 1279-1284 https://doi.org/10.1016/j.biortech.2008.08.045
  14. McMillan, J. D. (1992) Processes for pretreating lignocellulosic biomass: a review. NREL/TP-421-4978. NREL. Golden. CO
  15. National Renewable Energy Laboratory, Standard Biomass Analytical Procedures. www.nrel.gov/biomass/analytical_procedures.html
  16. Schwietzke, S., M. Ladisch, L. Russo, K. Kwant, T. Makinen, B. Kavalov, K. Maniatis, R. Zwart, G. Shahnan, K. Sipila, P. Grabowski, B. Telenius, M. White, and A, Brown. 2008. Analysis and identification of gaps in research for production of second-generation liquid transportation biofuels. Task 41. Project 2. IEA Bioenergy
  17. Shin, H. J, C.-J. Kim, and S. B. Kim. 2007. Optimization of culture medium for rifamycin SV production by Amycolatopsis mediterranei MM2 using statistical designs. Biotechnol. Bioprocess Eng. 12: 457-461 https://doi.org/10.1007/BF02931071
  18. Sun, F. and H. Chen. 2008. Enhanced enzymatic hydrolysis of wheat straw by aqueous glycerol pretreatment. Bioresour. Technol. 99: 6156-6161 https://doi.org/10.1016/j.biortech.2007.12.027
  19. Sun, F. and H. Chen. 2007. Evaluation of enzymatic hydrolysis of wheat straw pretreated by atmospheric glycerol autocatalysis. J. Chem. Technol. Biotechnol. 82: 1039-1044 https://doi.org/10.1002/jctb.1764
  20. Wyman, C. E., B. E. Dale, R. T. Elander, M. Holtzapple, M. R. Ladisch, and Y. Y. Lee. 2005. Coordinated development of leading biomass pretreatment technologies. Bioresour. Technol. 96: 1959-1966 https://doi.org/10.1016/j.biortech.2005.01.010
  21. Xu, Z., Q. Wang, Z. Jiang, X. Yang, and Y. Ji. 2007. Enzymatic hydrolysis of pretreated soybean straw. Biomass Bioenergy. 31: 162-167 https://doi.org/10.1016/j.biombioe.2006.06.015
  22. Yan, L., H. Zhang, J. Chen, Z. Lin, Q. Jin, H. Jia, and H. Huang. 2009. Dilute sulfuric acid cycle spray flow-through pretreatrnent of corn stover for enhancement of sugar recovery. Bioresour. Technol. 100: 1803-1808 https://doi.org/10.1016/j.biortech.2008.10.001
  23. Yat. S. C., A. Berger, and D. R. Shonnard. 2008. Kinetic characterization for dilute sulfuric acid hydrolysis of timber varieties and switchgrass. Bioresour. Technol. 99: 3855-3863 https://doi.org/10.1016/j.biortech.2007.06.046
  24. Zhu, J. Y., X. J. Pan, G. S. Wang, and R. Gleisner. 2009. Sulfite pretreatment (SPORL) for robust enzymatic saccharification of spruce and red pine. Bioresour. Technol. 100: 2411-2418 https://doi.org/10.1016/j.biortech.2008.10.057