Bioactivity of the Extract of Coptis chinensis: In-vitro Antifungal Activity against Phytophthora capsici and Growth-promotion Effect in Red-pepper

황련 추출물의 고추역병에 대한 In-vitro 항진균 활성 및 고추 생육촉진 효과

  • Ahn, Seon-Mi (Dept. of Food and Nutrition, Andong National University) ;
  • Lee, Dong-Sin (Dept. of Food and Nutrition, Andong National University) ;
  • Kim, Mi-Sun (Dept. of Food and Nutrition, Andong National University) ;
  • Choi, Su-Ji (Dept. of Food and Nutrition, Andong National University) ;
  • Choi, Chung-Sik (HansBio, Cyeongbuk Institute for Bioindustry) ;
  • Lee, Jung-Bok (HansBio, Cyeongbuk Institute for Bioindustry) ;
  • Jang, Han-Su (Gyeongbuk Institute for Bioindustry) ;
  • Sohn, Ho-Yong (Dept. of Food and Nutrition, Andong National University)
  • Published : 2009.09.28


To investigate anti-phytopathogenic fungal activity of Coptis chinensis, the methanol extract and its organic solvent fractions were prepared. Using the extract and the fractions, in-vitro spore-germination inhibition and mycelial-growth inhibition activities were evaluated against Colletotrichum gloeosporioides, Phytohpthora capsici, Pyricularia grisea, Rhizoctonia solani, Botryosphaeri dothidea, Glomerella cingulata, respectively. Treatment of the methanol extract (500 mg/mL) into the spore of phytopathogenic fungi completely inhibited germinations for 5 days, except B. dothidea, and showed strong antifungal activities against P. grisea and B. cinerea, and antioomycetes activity against P. capsici. The minimal growth inhibition concentrations of the methanol extract against P. grisea, B. cinerea and P. capsici were 300, 300, and 500 mg/mL, respectively. For practical application of C. chinensis in red-pepper field, the hot-water extract (1,000 mg/mL) was prepared in commercial facility, after evaluation of heat stability and solvent-extraction yields of antifungal substances. The 3-times leaf-spray of the extract from June to August, 2008 did not show any deleterious effect to red-pepper. In fact, the leaf-spray promoted plant growth including leaf, root and fruit. The average weight and rind of each fruit were increased to 119% and 117% comparison to those of without treatments. Our results suggest that C. chinensis is a useful source for control of red-pepper diseases and plant growth.

황련의 식물병원성 곰팡이에 대한 항진균 활성을 평가하기 위해 황련 메탄올 추출물 및 유기용매 분획물들을 조제하여 Colletotrichum gloeosporioides(고추탄저 병), Phytohpthora capsici(고추역병), Pyricularia grisea(벼도열병), Rhizoctonia solani(잎집무늬마름병), Botryosphaeri dothidea(겹무늬썩음 병), Glomerella cingulata(사과과실 탄저병) 및 Botrytis cinerea(잿빛곰팡이병)의 7종에 대해 $0{\sim}1,000{\mu}g/mL$ 농도로 처리한 후, 포자발아억제 활성 및 균사체 생육억제 활성을 평가하였다. 그 결과 황련 메탄올 추출물은 $500{\mu}g/mL$ 농도에서 B. dothidea를 제외한 6종의 곰팡이의 포자발아를 억제하였으며, P. grisea 및 B. cinerea에 대해 $300{\mu}g/mL$의 MIC를, P. capsici에 대해서는 $500{\mu}g/mL$의 MIC를 나타내었고, 다른 4종에 곰팡이에 대해서는 균사체 성장억제 활성이 나타나지 않았다. 고추 재배지의 실제적 적용을 위해 대량 추출조건을 선정한 후, 상업적 시설에서 열수 추출물($1,000{\mu}g/mL$)을 조제하고, 이를 6월부터 한달 간격으로 3회에 걸쳐 엽면살포한 결과 병해와 약해가 전혀 나타나지 않았다. 황련 살포에 의해 고추의 전반적인 생육, 잎, 뿌리 발육이 촉진되었으며, 고추는 평균중량이 119%, 과육의 두께가 평균 117% 증가되어 생육촉진 및 수확량 증가효과를 확인하였다. 본 연구결과는 황련 추출물이 고추 관련 질병 방제에 유용하며, 고추생육 촉진효과를 가짐을 시사하고 있다.



  1. An B. J. J. T. Lee, C. E. Lee, J. H. Kim, J. H. Son, J. H. Kwak, J. Y. Lee, T. S. Park, H. J. Bae, M. J. Jang, and C. H. Jo. 2005. A study of physiological activities of Coptis rhizoma and application for cosmetic ingredients. Kor. J. Herbology 20: 83-92
  2. Bae, J. H. 2005. Antimicrobial effect of Plagiorhegama dubium extract on food borne pathogen. Kor. J. Food Nutr. 18: 81-87
  3. Choi, G. J., J. C. Kim, K. S. Jang, H. K. Lim, I. K. Park, S. C. Shin, and K. Y. Cho. 2006. In vivo antifungal activities of 67 plant fruit extracts against six plant pathogenic fungi. J. Microbiol. Biotechnol. 16: 491-495
  4. Chung, I. M., and S. B. Paik. 1997. Separation and activity test of antifungal substance from C. japonica extract. Anal. Sci. Technol. 10: 153-159
  5. Hwang T. M., H. C. Kuo, T. H. Tseng, J. Y. Liu, and C. Y. Chu. Berberine induces apoptosis through a mitochondria caspases pathway in human hepatoma cells. 2006. Arch. Toxicol. 80: 62-73
  6. Jung, H. A., N. Y. Yoon, H. J. Bae, B. S. Min, and J. S. Choi. 2008. Inhibitory activitìes of the alkaloids from Coptidis Rhizoma against aldose reductase. Arch. Pharm. Res. 31: 1405-1412
  7. Jung, H. W., and Y. K. Park. 2007. Effects of subfractions of Coptidis rhizoma extract on the nitric oxide production in LPS-stimulated BV2 microglial cells. Kor. J. Herbology 22: 73-78
  8. Keumann V., Kosfalova D., Jantova S., Cemakova M., Drimal J. 2004. In vitro cytotoxicity of berberine against HeLa and L1210 cancer cell lines. Pharmazie 59: 548-551
  9. Kim, I. C. 2008. Antioxidative property and whitening effect of the Puerariaradix, Poriacocos and Coptidis rhizoma. J. Kor. Oil Chem. Soc. 25: 219-225
  10. Kim, J. Y., Y. S. Yi, and Y. H. Lim. 2009. Biological and antifungal activity of herbal plant extracts against Candida species. Kor. J. Microbiol. Biotechnol. 37: 42-48
  11. Lee, C. H., H. J. Lee, J. H. Jeon, and H. S. Lee. 2005. In vivo antifungal effect of Coptis japonica root-derived isoquinoline alkaloids against phytopathogenic fungi. J. Microbiol. Biotechnol. 15: 1402-1407
  12. Lee, J. B., J. H. Shin, J. O. Jang, K. S. Shin, C. S. Choi, K. W. Kim, M. S. Jo, C. P. Jeon, Y. H. Kim, and G. S. Kwon. 2008. Antifungal activity of Bacillus sp. AM-651 against Phytophthora capsici Kor. J. Microbiol. Biotechnol. 36: 227-232
  13. Lee, J. Y., D. H. Sherman, and B. K. Hwang. 2008. In vitro antimicrobial and in vivo antioomycete activities of the novel antibiotic thiobutacin. Pest Manag. Sci. 64: 172-177
  14. Liu, Z., Q, Liu, B. Xu, J. Wu, C. Guo, F. Zhu, Q. Yang, G. Gao, Y. Gong, and C. Shao. 2009. Berberine induces p53 dependent cell cycle arrest and apoptosis of human osteosarcoma cells by inflicting DNA damage. Mutat. Res. 662: 75-83
  15. Ma, C.Y., S. C. Shen, D. W. Huang, H. M. Chang, and J. S. Wu. 2008. Growth inhibition and induction of apoptosis in U937 cells by Coptis chinesis extract. J. Food Sci. 73: H127-133
  16. Min B. S. and J. S. Cho. 2008. Quantitative determination of protoberberines from the roots of Coptis chinensis. Nat. Prod. Sci. 14: 1-5
  17. Park, Y. K., H. W. Jung, C. M. Kim, J. S. Choi, and Y. S. Kim. 2007. Effect of berberine on the production of inflammatory mediators from LPS-stimulated BN2 microglial cells. Kor. J. Herbology 22: 117-125
  18. Ryu, H. Y., E. J. Kum, K. H. Bae, Y. K. Kim, I. S. Kwon, and H. Y. Sohn. 2007. Evaluation for the antimicrobial, antioxidant and antithrombosis activity of Korean traditional liquors. Kor. J. Microbiol. Biotechnol. 35: 310-315
  19. Sohn, H. Y. H. Y. Ryu, Y. Jang, H. S. Jang, Y. M. Park, and S. Y. Kim. 2008. Evaluation of antimicrobial, antithrombin, and antioxidant activity of aerial part of Saxifraga stolonifera. Kor. J. Microbiol. Biotechnol. 36: 195-200
  20. Sohn, H. Y., K. H. Son, C. S. Kwon, G. S. Kwon, and S. S. Kang. 2004. Antimicrobial and cytotoxic activity of 18 prenylated flavonoids isolated from medicinal plants: Morus alba L., Morus mongolica Schneider, Broussnetia papyrifera (L.) Vent, Sophora flavescens Ait and Echinosophora koreensis Nakai. Phytomedicine 11: 666-672
  21. Tanabe, H., H. Suzuki, A. Nagatsu, H. Mizukarni, Y. Ogihara, and M. Inoue. 2006. Selective inhibition of vascular smooth muscle cell proliferation by coptisine isolated from Coptis rhizome, one of the crude drugs composing Kampo medicines Unsei-in. Phytomedicine 13: 334-342
  22. Tang, L. Q., W. Wei, L. M. Chen, and S. Liu. 2006. Effects of berberine on diabetes induced by allxan and a high-fat/high-cholesterol diet in rats. J. Ethnopharmacol. 108: 109-115
  23. Yan, D., C. Jin, X. Xiao, and X. Dong. 2008. Antimicrobial properties of berberine alkaloids in Coptis chinensis Franch by microcalorimetry. J. Biochem. Biophys. Methods 70: 845-849
  24. Yoo, J. K., K. H. Ryu, J. H. Kwon, S. S. Lee, and Y. J. Ahn. 1998. Fungicidal activity of oriental medicinal plant extracts against plant pathogenic fungi. Agric. Chem. Biotechnol. 41: 600-604
  25. Yu, H. H., K. J. Kim, J. D. Cha, H. K. Kim, Y. E. Lee, N. Y. Choi, and Y. O. You. 2005. Antimicrobial activity of berberine alone and in combination with ampicillin or oxacillin against methicillin-resistant Staphylococcus aureus. J. Med. Food 8: 454-461