Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Development and Characterization of New Microsatellite Markers for the Oyster Mushroom (Pleurotus ostreatus)

  • Ma, Kyung-Ho (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Lee, Gi-An (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Lee, Sok-Young (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Gwag, Jae-Gyun (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Kim, Tae-San (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Kong, Won-Sik (Mushroom Research Division, National Institute Horticultural and Herbal Science, RDA) ;
  • Seo, Kyoung-In (Mushroom Research Division, National Institute Horticultural and Herbal Science, RDA) ;
  • Lee, Gang-Seob (Genomics Division, National Academy of Agricultural Science, RDA) ;
  • Park, Yong-Jin (Department of Plant Resoures, College of Industrial Sciences, National Kongju University)
  • Published : 2009.09.30
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Abstract

We developed and characterized 36 polymorphic microsatellite markers for the oyster mushroom (Pleurotus ostreatus). In total, 169 alleles were identified with an average of 4.7 alleles per locus. Values for observed ($H_o$) and expected ($H_E$) heterozygosities ranged from 0.027 to 0.946 and from 0.027 to 0.810, respectively. Nineteen loci deviated from Hardy-Weinberg equilibrium. Significant (P<0.05) excess heterozygosity was observed at nine loci. Linkage disequilibrium (LD) was significant (P<0.05) between pairs of locus alleles. Cluster analysis revealed that five species of genus Pleurotus made a distinct group, and the individual cultivars were grouped into major five groups from G-1 to G-5. The diverse cultivars of P. ostreatus were discriminated and the other four species revealed a different section in the UPGMA tree. These microsatellite markers proved to be very useful tools for genetic studies, including assessment of the diversity and population structure of P. ostreatus.

Keywords

References

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