Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Abundance and Structure of Microbial Loop Components (Bacteria and Protists) in Lakes of Different Trophic Status

  • Chrost, Ryszard J. (Department of Microbial Ecology, Faculty of Biology, University of Warsaw) ;
  • Tomasz, Adamczewski (Department of Microbial Ecology, Faculty of Biology, University of Warsaw) ;
  • Kalinowska, Krystyna (Hydrobiological Station, Centre for Ecological Research, Polish Academy of Sciences) ;
  • Skowronska, Agnieszka (Department of Environmental Microbiology, Faculty of Environmental Sciences and Fisheries, University of Warmia and Mazury)
  • Published : 2009.09.30
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Abstract

The abundance, biomass, size distribution, and taxonomic composition of bacterial and protistan (heterotrophic and autotrophic nanoflagellates and ciliates) communities were investigated in six lakes of Masurian Lake District (north-eastern Poland) differing in trophic state. Samples were taken from the trophogenic water layer during summer stratification periods. Image analysis techniques with fluorescent in situ hybridization (FISH) as well as [$^3H$]-methyl-thymidine incorporation methods were applied to analyze differences in the composition and activity of bacterial communities. The greatest differences in trophic parameters were found between the humic lake and remaining non-humic ones. The same bacterial and heterotrophic nanoflagellate (HNF) cell size classes dominated in all the studied lakes. However, distinct increases in the contributions of large bacterial (>$1.0{\mu}m$) and HNF (>$10{\mu}m$) cells were observed in eutrophic lakes. The bacterial community was dominated by the ${\beta}$-Proteohacteria group, which accounted for 27% of total DAPI counts. Ciliate communities were largely composed of Oligotrichida. Positive correlations between bacteria and protists, as well as between nanoflagellates (both heterotrophic and autotrophic) and ciliates, suggest that concentrations of food sources may be important in determining the abundance of protists in the studied lakes.

Keywords

References

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