Atromentin-Induced Apoptosis in Human Leukemia U937 Cells

  • Kim, Jin-Hee (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Lee, Choong-Hwan (Department of Bioscience and Biotechnology, Konkuk University)
  • Published : 2009.09.30

Abstract

In the course of screening for apoptotic substances that induce apoptosis in human leukemia U937 cells, a fungal strain, F000487, which exhibits potent inducible activity, was selected. The active compound was purified from an ethyl acetate extract of the microorganism by Sep-pak $C_{18}$ column chromatography and HPLC, and was identified as atromentin by spectroscopic methods. This compound induced caspase-3 processing in human leukemia U937 cells. The caspase-3 and poly (ADP-ribose) polymerase (PARP) were induced by atromentin in a dose-dependent manner. Furthermore, DNA fragmentation was also induced by this compound in a dose-dependent manner. These results show that atromentin potently induces apoptosis in U937 cells and that atromentin-induced apoptosis is related to the selective activation of caspases.

Keywords

References

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