Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Induction of IL-8 and reactive oxygen species in periodontal ligament cells by Aggregatibacter actinomycetemcomitans

치주인대세포에서 Aggregatibacter actinomycetemcomitans의 IL-8 및 활성산소종 유도능

  • Lee, Yang-Sin (Department of Oral Biology, College of Dentistry, Yonsei University) ;
  • Park, Hong-Gyu (Department of Oral Biology, College of Dentistry, Yonsei University) ;
  • Kim, Sung-Whan (Department of Applied Life Science, The Graduate School, Yonsei University) ;
  • Cha, Jeong-Heon (Department of Oral Biology, College of Dentistry, Yonsei University) ;
  • Yoo, Yun-Jung (Department of Oral Biology, College of Dentistry, Yonsei University)
  • 이양신 (연세대학교 치과대학 구강생물학교실) ;
  • 박홍규 (연세대학교 치과대학 구강생물학교실) ;
  • 김성환 (연세대학교 대학원 응용생명과학과) ;
  • 차정헌 (연세대학교 치과대학 구강생물학교실) ;
  • 유윤정 (연세대학교 치과대학 구강생물학교실)
  • Published : 2009.09.30
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Abstract

Purpose: Interleukin (IL)-8 is one of pro-inflammatory cytokines. Reactive oxygen species (ROS) are reduced metabolites of $O_2$. Aggregatibacter actinomycetemcomitans is one of representative periodontopathogens. To investigate the role of A. actinomycetemcomitans in IL-8 expression of periodontal ligament (PDL) cells, we estimated the production of IL-8 and ROS in A. actinomycetemcomitans treated PDL cells. Methods: The IL-8 production was determined by enzyme-linked immunosorbent assay. The ROS production was estimated using H2DCFDA and FACS. Results: A. actinomycetemcomitans increased the production of IL-8 and ROS at 10, 100, and 500 multiplicity of infection. N-acetylcysteine, an antioxidant of ROS, down-regulated the production of IL-8 induced by A. actinomycetemcomitans. Conclusions: These results suggest that A. actinomycetemcomitans induces IL-8 production and ROS may act as a mediator in this process.

Keywords

References

  1. Ishikawa I. Host responses in periodontal diseases: a preview. Periodontol 2000 2007;43:9-13 https://doi.org/10.1111/j.1600-0757.2006.00188.x
  2. Baggiolini M, Clark-Lewis I. Interleukin-8, a chemotactic and inflammatory cytokine. FEBS Lett 1992;307:97-101 https://doi.org/10.1016/0014-5793(92)80909-Z
  3. Tanaka S. Signaling axis in osteoclast biology and therapeutic targeting in the RANKL/RANK/OPG system. Am J Nephrol 2007;27:466-478' https://doi.org/10.1159/000106484
  4. Bendre MS, Montague DC, Peery T, et al. Interleukin-8 stimulation of osteoclastogenesis and bone resorption is a mechanism for the increased osteolysis of metastatic bone disease. Bone 2003;33:28-37 https://doi.org/10.1016/S8756-3282(03)00086-3
  5. Bendre MS, Margulies AG, Walser B, et al. Tumor-derived interleukin-8 stimulates osteolysis independent of the receptor activator of nuclear factor-κB ligand pathway. Cancer Res 2005;65:11001-11009 https://doi.org/10.1158/0008-5472.CAN-05-2630
  6. Tsai CC, Ho YP, Chen CC. Levels of interleukin-1 $\beta$ and interleukin-8 in gingival crevicular fluids in adult periodontitis. J Periodontol 1995;66:852-859 https://doi.org/10.1902/jop.1995.66.10.852
  7. Gamonal J, Acevedo A, Bascones A, Jorge O, Silva A. Levels of interleukin-1 β, -8, and -10 and RANTES in gingival crevicular fluid and cell populations in adult periodontitis patients and the effect of periodontal treatment. J Periodontol 2000;71:1535-1545 https://doi.org/10.1902/jop.2000.71.10.1535
  8. Gamonal J, Acevedo A, Bascones A, Jorge O, Silva A. Characterization of cellular infiltrate, detection of chemokine receptor CCR5 and interleukin-8 and RANTES chemokines in adult periodontitis. J Periodontal Res 2001;36:194-203 https://doi.org/10.1034/j.1600-0765.2001.360309.x
  9. Socransky SS, Haffajee AD. Periodontal microbial ecology. Periodontol 2000 2005;38:135-187 https://doi.org/10.1111/j.1600-0757.2005.00107.x
  10. Yamaji Y, Kubota T, Sasaguri K, et al. Inflammatory cytokine gene expression in human periodontal ligament fibroblasts stimulated with bacterial lipopolysaccharides. Infect Immun 1995;63:3576-3581
  11. Yamamoto T, Kita M, Oseko F, et al. Cytokine production in human periodontal ligament cells stimulated with Porphyromonas gingivalis. J Periodontal Res 2006;41:554-559 https://doi.org/10.1111/j.1600-0765.2006.00905.x
  12. Lee SH, Kim KK, Choi BK. Upregulation of intercellular adhesion molecule 1 and proinflammatory cytokines by the major surface proteins of Treponema maltophilum and Treponema lecithinolyticum, the phylogenetic group IV oral spirochetes associated with periodontitis and endodontic infections. Infect Immun 2005;73:268-276 https://doi.org/10.1128/IAI.73.1.268-276.2005
  13. Patil C, Rossa C, Jr., Kirkwood KL. Actinobacillus actinomycetemcomitans lipopolysaccharide induces interleukin-6 expression through multiple mitogen-activated protein kinase pathways in periodontal ligament fibroblasts. Oral Microbiol Immunol 2006;21:392-398 https://doi.org/10.1111/j.1399-302X.2006.00314.x
  14. Droge W. Free radicals in the physiological control of cell function. Physiol Rev 2002;82:47-95 https://doi.org/10.1152/physrev.00018.2001
  15. Lakshminarayanan V, Beno DW, Costa RH, Roebuck KA. Differential regulation of interleukin-8 and intercellular adhesion molecule-1 by H2O2 and tumor necrosis factor-α in endothelial and epithelial cells. J Biol Chem 1997;272:32910-32918 https://doi.org/10.1074/jbc.272.52.32910
  16. Verhasselt V, Goldman M, Willems F. Oxidative stress up-regulates IL-8 and TNF-$\alpha$ synthesis by human dendritic cells. Eur J Immunol 1998;28:3886-3890 https://doi.org/10.1002/(SICI)1521-4141(199811)28:11<3886::AID-IMMU3886>3.0.CO;2-M
  17. Josse C, Boelaert JR, Best-Belpomme M, Piette J. Importance of post-transcriptional regulation of chemokine genes by oxidative stress. Biochem J 2001;360:321-333 https://doi.org/10.1042/0264-6021:3600321
  18. Hwang YS, Jeong M, Park JS, et al. Interleukin-1$\alpha$ stimulates IL-8 expression through MAP kinase and ROS signaling in human gastric carcinoma cells. Oncogene 2004;23:6603-6611 https://doi.org/10.1038/sj.onc.1207867
  19. Pelaia G, Cuda G, Vatrella A, et al. Effects of hydrogen peroxide on MAPK activation, IL-8 production and cell viability in primary cultures of human bronchial epithelial cells. J Cell Biochem 2004;93:142-152 https://doi.org/10.1002/jcb.20124
  20. Lee YS, Bak EJ, Kim M, et al. Induction of IL-8 in periodontal ligament cells by $H_2O_2$. J Microbiol 2008;46:579-584 https://doi.org/10.1007/s12275-008-0182-3
  21. Seo JY, Kim H, Kim KH. Transcriptional regulation by thiol compounds in Helicobacter pylori-induced interleukin-8 production in human gastric epithelial cells. Ann N Y Acad Sci 2002;973:541-545 https://doi.org/10.1111/j.1749-6632.2002.tb04697.x
  22. Christersson LA, Albini B, Zambon JJ, Wikesjo UM, Genco RJ. Tissue localization of Actinobacillus actinomycetemcomitans in human periodontitis. I. Light, immunofluorescence and electron microscopic studies. J Periodontol 1987;58:529-539 https://doi.org/10.1902/jop.1987.58.8.529
  23. Christersson LA, Wikesjo UM, Albini B, Zambon JJ, Genco RJ. Tissue localization of Actinobacillus actinomycetemcomitans in human periodontitis. II. Correlation between immunofluorescence and culture techniques. J Periodontol 1987;58:540-545 https://doi.org/10.1902/jop.1987.58.8.540
  24. Barnes PJ, Karin M. Nuclear factor-κB: a pivotal transcription factor in chronic inflammatory diseases. N Engl J Med 1997;336:1066-1071 https://doi.org/10.1056/NEJM199704103361506
  25. Meyer M, Schreck R, Baeuerle PA. H2O2 and antioxidants have opposite effects on activation of NF-κ B and AP-1 in intact cells: AP-1 as secondary antioxidant-responsive factor. Embo J 1993;12:2005-2015
  26. DeForge LE, Preston AM, Takeuchi E, et al. Regulation of interleukin 8 gene expression by oxidant stress. J Biol Chem 1993;268:25568-25576
  27. O'Hara AM, Bhattacharyya A, Bai J, et al. Tumor necrosis factor (TNF)-α-induced IL-8 expression in gastric epithelial cells: role of reactive oxygen species and AP endonuclease-1/redox factor (Ref)-1. Cytokine 2009;46:359-369 https://doi.org/10.1016/j.cyto.2009.03.010
  28. Kim do Y, Jun JH, Lee HL, et al. N-acetylcysteine prevents LPS-induced pro-inflammatory cytokines and MMP2 production in gingival fibroblasts. Arch Pharm Res 2007;30:1283-1292 https://doi.org/10.1007/BF02980269
  29. Di Paola R, Mazzon E, Zito D, et al. Effects of Tempol, a membrane-permeable radical scavenger, in a rodent model periodontitis. J Clin Periodontol 2005;32:1062-1068 https://doi.org/10.1111/j.1600-051X.2005.00818.x
  30. Toker H, Ozdemir H, Eren K, Ozer H, Sahin G. N-acetylcysteine, a thiol antioxidant, decreases alveolar bone loss in experimental periodontitis in rats. J Periodontol 2009;80:672-678 https://doi.org/10.1902/jop.2009.080509
  31. Long P, Hu J, Piesco N, Buckley M, Agarwal S. Low magnitude of tensile strain inhibits IL-1$\alpha$-dependent induction of pro-inflammatory cytokines and induces synthesis of IL-10 in human periodontal ligament cells in vitro. J Dent Res 2001;80:1416-1420 https://doi.org/10.1177/00220345010800050601
  32. Lee HJ, Cho JW, Kim SC, et al. Roles of p38 and ERK MAP kinases in IL-8 expression in TNF-$\alpha$- and dexamethasone-stimulated human periodontal ligament cells. Cytokine 2006;35:67-76 https://doi.org/10.1016/j.cyto.2006.07.009