Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Response of fetal rat calvarial cells on mineral trioxide aggregate after IL-$1{\beta}$ stimulation

IL-$1{\beta}$ 처리 백서 두개관 세포의 mineral trioxide aggregate에 대한 반응

  • Lee, Sool-Heon (Department of Periodontology, School of Dentistry, Dental Science Reserch Institute, Chonnam National University) ;
  • Park, Ji-Il (Department of Dental hygiene, Gwangju Health College) ;
  • Kim, Young-Joon (Department of Periodontology, School of Dentistry, Dental Science Reserch Institute, Chonnam National University)
  • 이술현 (전남대학교 치의학전문대학원 치주과학교실, 전남대학교 치의학연구소) ;
  • 박지일 (광주보건전문대학 치위생과) ;
  • 김영준 (전남대학교 치의학전문대학원 치주과학교실, 전남대학교 치의학연구소)
  • Published : 2009.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: The purpose of this study was to investigate the ability of Mineral trioxide aggregate(MTA) to support osteoclastic differentiation from fetal rat calvarial cell. Methods: In this study, response of IL-6, RANKL, and OPG in fetal rat calvarial cells stimulated with IL-$1{\beta}$ on MTA was evaluated by ELISA and RT-PCR. Results: The results were as follows; there was no significant difference between glass and MTA at 5days. In ELISA analysis, Glass group and MTA group showed similar IL-6 expression, Glass+IL-$1{\beta}$ group and MTA+IL-$1{\beta}$ group showed similar IL-6 expression. In RT-PCR analysis, Glass group and MTA group showed similar IL-6, RANKL, OPG mRNA expression, MTA+IL-$1{\beta}$ group and Glass+IL-$1{\beta}$ group showed 3 fold increase of IL-6 and RNAKL mRNA expression when compared with MTA group. All groups showed similar OPG mRNA expression. Conclusions: MTA does not suppress cell proliferation and increase the proinflammatory cytokine that induce osteoclastogenesis. Thus, MTA is biocompatible material that could be used in various clinical conditions.

Keywords

References

  1. Torabinejad M, Hong CU, McDonald F, Pitt Ford TR. Physical and chemical properties of a new root-end filling material. J Endod 1995;21:349-353 https://doi.org/10.1016/S0099-2399(06)80967-2
  2. Koh ET, McDonald F, Pitt Ford TR, Torabinejad M. Cellular response to Mineral Trioxide Aggregate. J Endod 1998;24:543-547 https://doi.org/10.1016/S0099-2399(98)80074-5
  3. Robert HW, Toth JM, Berzins DW, Charlton DG. Mineral trioxide aggregate material use in endodontic treatment: a review of the literature. Dent Mater 2008;24:149-164 https://doi.org/10.1016/j.dental.2007.04.007
  4. Zhu Q, Haglund R, Safavi KE, Spangberg LSW. Adhesion of human osteoblasts on root-end filling materials. J Endod 2000;26:404-406 https://doi.org/10.1097/00004770-200007000-00006
  5. Torabinejad M, Hong CU, Pitt Ford TR, Kettering JD. Cytotoxicity of four root-end filling materials. J Endod 1995;21:489-492 https://doi.org/10.1016/S0099-2399(06)80518-2
  6. Pelliccioni GA, Ciapetti G, Cenni E, Granchi D, Nanni M, Pagani S, Giunti A. Evaluation of osteoblast-like cell response to ProRoot MTA cement. J Mat Sci 2004;15:167-173
  7. Yavuzyilmaz E, Yamalik N, Bulut S, Ozen S, Ersoy F, Saatci U. The gingival crevicular fluid interleukin-1beta and tumor necrosis factor-a levels in patients with rapidly progressive periodontitis. Aust Dent J 1995;40:46-49 https://doi.org/10.1111/j.1834-7819.1995.tb05614.x
  8. Tsai CC, Hou YP, Chen CC. Levels of interleukin-1beta and interleukin-8 in gingival crevicular fluid in adult periodontitis. J Periodontol 1995;66:852-859 https://doi.org/10.1902/jop.1995.66.10.852
  9. Ishihara Y, Kuroyagani T, Shirozi N, Yamagishi E, Ohguchi M, Koide M, Ueda N, Amano K, Noguchi T. Gingival crevicular interleukin-1 and interleukin-1 receptor antagonist levels in periodontally healthy and diseased sites. J Periodont Res 1997;32:524-529 https://doi.org/10.1111/j.1600-0765.1997.tb00568.x
  10. Giannopoulou C, Kama JJ, Mombelli A. Effect of inflammation, smoking, and stress on gingival, crevicular fluid cytokine level. Journal of Clinical Periodontology 2003;30:145-153 https://doi.org/10.1034/j.1600-051X.2003.300201.x
  11. Barrieshi-Nusair K, Qudeimat MA. A prospective clinical study of mineral trioxide aggregate for partial pulpotomy in cariously exposed permanent teeth. J Endod 2006;32:731-735 https://doi.org/10.1016/j.joen.2005.12.008
  12. Cory AH, Barltrop JA, Cory JG. Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture. Cancer Commun 1991;3:207-212
  13. Nakayama A, Ogiso B, Tanabe N, Takeichi O, Matsuzaka K, Inoue T. Behaviour of bone marrow osteoblasts-like cells on mineral trioxide aggregate: morphology and expression of type 1 collagen and bone-related protein mRNAs. Int Endodont J 2005;38:203-210 https://doi.org/10.1111/j.1365-2591.2004.00917.x
  14. Nakashima T, Kobayashi Y, Yamasaki S, Kawakami A, Eguchi K, Sasaki H, Sakai H. Protein expression and functional difference of membrane-bound and soluble receptor activator of NF-kappaB ligand : Modulation of the expression by osteotropic factors and cytokines. Biochem Biophys Res Commun 2000;275:768-775 https://doi.org/10.1006/bbrc.2000.3379
  15. Hofbaur LC, Heufelder AE. Role of receptor activator of nuclear factor-kB ligand and osteoprotegerin in bone cell biology. J Mol Med 2001;79:243-253 https://doi.org/10.1007/s001090100226
  16. Siggelkow H, Eidner T, Lehmann G, Viereck V, Raddatz D, Munzel U, Hein G, Hufner M. Cytokine, osteoprotegerin, and RANKL in vitro and histomorphometric indices of bone turnover in patients with different bone disease. J Bone Miner Res 2003;18:529-538 https://doi.org/10.1359/jbmr.2003.18.3.529
  17. Kobayashi N, Kadono Y, Naito A, Matsumoto K, Yamamoto T, Tanaka S, Inoue J. Segregation of TRAF6-mediated signaling pathways clarifies its role in osteoclastogenesis. EMBO J 2001;20:1271-1280 https://doi.org/10.1093/emboj/20.6.1271
  18. Lerner UH. Inflammation-induced bone remodeling in periodontal disease and the influence of post menopausal osteoporosis. J Dent Res 2006;85:596-607 https://doi.org/10.1177/154405910608500704
  19. Boyle WJ, Simonet WS, Lacey DL. Osteoclast differentiation and activation. Nature 2003;423:337-342 https://doi.org/10.1038/nature01658
  20. Cochran DL. Inflammation and bone loss in periodontal disease. J Periodontol. 2008;79(Suppl):1569-1576 https://doi.org/10.1902/jop.2008.080233
  21. Ishihara K, Hirano T. IL-6 in autoimmune disease and chronic inflammatory proliferative disease. Cytoline Growth Factor Rev 2002;13:357-368 https://doi.org/10.1016/S1359-6101(02)00027-8
  22. Dalrymple SA, Slattery R, Aud DM, Krishna M, Lucan LA, Murray R. Interleukin-6 is required for a protective immune response to systemic Escherichia coli infection. Infect Immun 1996;64:3231-3235