Mycobiology

The Korean Society of Mycology (한국균학회)
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Production of Blastospore of Entomopathogenic Beauveria bassiana in a Submerged Batch Culture

  • Pham, Tuan Anh (Department of Bioscience and Biotechnology, The University of Suwon) ;
  • Kim, Jeong-Jun (Applied Entomology Division, National Academy of Agricultural Science) ;
  • Kim, Seon-Gon (Environment-Friendly Agricultural Research Institute, Jeollanam-do Agricultural Research & Extension Services) ;
  • Kim, Keun (Department of Bioscience and Biotechnology, The University of Suwon)
  • Published : 2009.09.30
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Abstract

The principal objective of this study was to determine the optimal liquid culture conditions in shake flasks for maximal sporulation of Beauveria bassiana. The optimal initial pH for the spore production of B. bassiana using Potato Dextrose Broth was 5.2. The screening in shake flasks of carbon and nitrogen sources resulted in the identification of an optimal medium based on 3% sucrose and 1% casamino acid, with a C : N ratio of 22 : 4. Using this medium, a production level of $5.65{\times}10^7$ spores per ml was obtained after 5 days of culture. Using 3% corn meal, 2% corn steep powder, and 2% rice bran, the maximum spore concentration of $8.54{\times}10^8$/ml was achieved 8 days after inoculation at $25^{\circ}C$ in a rotary shaking incubator operated at 200 rpm. This represents a yield gain of approximately 2.89 times that of pre-optimization.

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References

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