Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Allergenicity Assessment of Cry Proteins in Insect-resistant Genetically Modified Maize Bt11, MON810, and MON863

  • Kim, Jae-Hwan (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Seo, Young-Ju (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim, Ji-Young (Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine) ;
  • Han, Young-Shin (Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine) ;
  • Lee, Kwang-Shin (Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine) ;
  • Kim, Sun-Ah (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim, Han-Na (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Ahn, Kang-Mo (Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine) ;
  • Lee, Sang-Il (Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine) ;
  • Kim, Hae-Yeong (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University)
  • Published : 2009.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study aimed to evaluate the potential allergenicity of Cry proteins in insect-resistant genetically modified (GM) maizes (Bt11, MON810, and MON863) using serum screening tests. Serum samples were obtained from Korean children (0-15 years old) with allergic symptoms who had positive maize-specific IgE. The levels of serum specific IgE was measured by the Phadia ImmunoCAP system and considered as positive when they are 0.35 kU/L or higher. Cry proteins (Cry1Ab in Bt11, mCry1Ab in MON810, and Cry3Bb1 in MON863) were expressed in Escherichia coli and purified for serum screening. The reactivity of purified Cry proteins was confirmed by IgE immunoblots in 50 patients (maize-sensitized patients). There was no reaction between Cry proteins and sera from maize-sensitized patients. Our results suggest that these Cry proteins are not likely to cause allergic reactions. Further studies using more sera from patients with true clinical allergies are needed to evaluate the potential allergenicity of novel proteins in GM maize.

Keywords

References

  1. Kim JH, Kim HY. Allergenicity of genetically modified crops. Pediatr. Allergy Respir. Dis. 17: 166-172 (2007)
  2. James C. Global Status of Commercialized Biotech/GM Crops: ISAAA Briefs No. 39. International Service for the Acquisition of Agri-biotech Applications (ISAAA), Ithaca, NY, USA. pp. 1-19 (2008)
  3. Agriculture & Biotechnology Strategies. Agbios database. Available from: http://www. Agbios.com. Accessed Mar. 13, 2009
  4. Korea Biosafety Clearing House. Approval status of LMO in Korea (By Feb. 28. 2009). Available from: http://www.biosafety.or.kr. Accessed Mar. 13, 2009
  5. Metcalfe DD. Genetically modified crops and allergenicity. Nat. Immunol. 6: 857-860 (2005) https://doi.org/10.1038/ni0905-857
  6. Goodman RE, Hefle SL, Taylor SL, van Ree R. Assessing genetically modified crops to minimize the risk of increased food allergy: A review. Int. Arch. Allergy Imm. 137: 153-166 (2005) https://doi.org/10.1159/000086314
  7. Spok A, Gaugitsch H, Laffer S, Pauli G, Saito H, Sampson H, Sibanda E, Thomas W, van Hage-Hamsten M, Valenta R. Suggestions for the assessment of the allergenic potential of genetically modified organisms. Int. Arch. Allergy Imm. 137: 167-180 (2005) https://doi.org/10.1159/000086315
  8. Goodman RE, Vieths S, Sampson HA, Hill D, Ebisawa M, Taylor SL, van Ree R. Allergenicity assessment of genetically modified crops-what makes sense? Nat. Biotechnol. 26: 73-81 (2008) https://doi.org/10.1038/nbt1343
  9. Hileman RE, Silvanovich A, Goodman RE, Rice EA, Holleschak G, Astwood JD, Hefle SL. Bioinformatic methods for allergenicity assessment using a comprehensive allergen database. Int. Arch. Allergy Imm. 128: 280-291 (2002) https://doi.org/10.1159/000063861
  10. Goodman RE. Performing IgE serum testing due to bioinformatics matches in the allergenicity assessment of GM crops. Food Chem. Toxicol. 46: S24-S34 (2008) https://doi.org/10.1016/j.fct.2008.07.023
  11. Codex Alimentarius Commission. Alinorm 03/04: Joint FAO/WHO Food standard programme. Codex alimentarius commission, 25th Session, Rome, Italy, 30 June-5 July, 2003. Appendix III, Guideline for the conduct of food safety assessment of foods derived from recombinant-DNA plants and Appendix IV, Annex on the assessment of possible allergenicity. pp. 47-60 (2003)
  12. Barber D, Rodríguez, Salcedo G. Molecular profiles: A new tool to substantiate serum banks for evaluation of potential allergenicity of GMO. Food Chem. Toxicol. 46: S35-S40 (2008) https://doi.org/10.1016/j.fct.2008.07.022
  13. Okunuki H, Teshima R, Shigeta T, Sakushima J, Akiyama H, Goda Y, Toyoda M, Sawada J. Increased digestibility of two products in genetically modified food (CP4-EPSPS and Cry1Ab) after preheating. J. Food Hyg. Soc. Jpn. 43: 68-73 (2002) https://doi.org/10.3358/shokueishi.43.68
  14. Batista R, Nunes, B, Carmo M, Cardoso C, São Josè H, Bugalho de Almeida A, Manique A, Bento L, Ricardo CP, Oliveira MM. Lack of detectable allergenicity of transgenic maize and soya sample. J. Allergy Clin. Imm. 116: 403-410 (2005) https://doi.org/10.1016/j.jaci.2005.04.014
  15. Nakajima O, Teshima R, Takagi K, Okunuki H, Sawada J. ELISA method for monitoring human serum IgE specific for Cry1Ab introduced into genetically modified corn. Regul. Toxicol. Pharm. 47: 90-95 (2007) https://doi.org/10.1016/j.yrtph.2006.08.003
  16. Herman RA, Schafer BW, Korjagin VA, Ernest AD. Rapid digestion of Cry34Ab1 and Cry35Ab1 in simulated gastric fluid. J. Agr. Food Chem. 51: 6823-6827 (2003) https://doi.org/10.1021/jf034290p