Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
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Effect of pinewood nematode on the water content and early disease development of seedlings of susceptible Pinus densiflora and resistant Pinus × rigitaeda for breeding for resistance to pinewood nematode

  • Woo, Kwan-Soo (Department of Forest Resources Development, Korea Forest Research Institute) ;
  • Yoon, Jun-Hyuck (Department of Forest Resources Development, Korea Forest Research Institute) ;
  • Fins, Lauren (Department of Forest Resources, University of Idaho) ;
  • Lee, Do-Hyung (Department of Forest Resources, Yeungnam University) ;
  • Koo, Yeong-Bon (Department of Forest Resources Development, Korea Forest Research Institute) ;
  • Yeo, Jin-Kie (Department of Forest Resources Development, Korea Forest Research Institute)
  • Received : 2009.12.07
  • Published : 20091200
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Abstract

Three-year-old seedlings of susceptible Pinus densiflora and resistant Pinus x rigitaeda were each inoculated with the pinewood nematode, Bursaphelenchus xylophilus, to compare disease development. Needle dehydration was evident on seedlings of P. densiflora by 20 days after inoculation, 10 days earlier than this symptom was observed on P. ${\times}$ rigitaeda. Xylem drying was more frequent in seedlings of P. densiflora than in that of P. ${\times}$ rigitaeda between 20 and 60 days after inoculation. No significant differences were found between P. densiflora and P. ${\times}$ rigitaeda for stem water content or for stem and leaf relative water content in current-year branches after nematode inoculation, but the average number of B. xylophilus recovered from stems differed significantly between the two groups. The number of B. xylophilus recovered from stems was negatively correlated with the stem water content and with stem and leaf relative water content. By the time the experiment was terminated at 60 days after inoculation, all 3 of the last group of P. densiflora seedlings had died, but 2 of the 3 remaining P. ${\times}$ rigitaeda hybrid seedlings were still alive. Additional studies are needed to further explore the specific mechanisms preventing nematode multiplication in the seedlings of resistant P. ${\times}$ rigitaeda.

Keywords

References

  1. Dropkin VH. 1985. Pine wilt in the U.S. In Proceedings of the United States - Japan Seminar: The resistance mechanisms of pines against pine wilt disease, May 7-11, 1984. Edited by V.H. Dropkin, University of Missouri–Columbia, Extension Publ. No. 116:3-5
  2. Futai K, Furuno T. 1979. The variety of resistances among pine-species to pinewood nematode, Bursaphelenchus lignicolus. Bull. Kyoto Univ. For. 51:23-36
  3. Hara N, Takeuchi Y. 2006. Histological analysis for mechanism of pine wilt disease. J. Jpn. For. Soc. 88:364-369 https://doi.org/10.4005/jjfs.88.364
  4. Ikeda T, Kiyohara T. 1995. Water relations, xylem embolism and histological features of Pinus thunbergii inoculated with virulent or avirulent pine wood nematode, Bursaphelenchus xylophilus. J. Exp. Bot. 46(285):441-449
  5. Ishii K, Kurinobu S, Ohba K, Furukoshi T. 1981. Resistance of interspecific hybrids among the pine species belonging to Sylvestres subsect. to pine wood nematode (Bursaphelenchus lignicolus). Annual Meeting of Japanese Forestry Society, 92nd, pp. 291-292. Tokyo: Jpn. For. Soc. pp. 536
  6. Jones JT, Moens M, Mota M, Li H, Kikuchi T. 2008. Bursaphelenchus xylophilus: opportunities in comparative genomics and molecular host-parasite interactions. Mol. Plant Pathol. 9(3):357-368 https://doi.org/10.1111/j.1364-3703.2007.00461.x
  7. Kuroda K. 1991. Mechanism of cavitation development in the pine wilt disease. Eur. J. For. Path. 21:82-89 https://doi.org/10.1111/j.1439-0329.1991.tb00947.x
  8. Kuroda K. 2004. Inhibiting factors of symptom development in several Japanese red pine [Pinus densiflora] families selected as resistant to pine wilt. J. For. Res. 9:217-224 https://doi.org/10.1007/s10310-004-0076-0
  9. Kuroda K, Yamada T, Ito S. 1991. Bursaphelenchus xylophilus- induced pine wilt: factors associated with resistance. Eur. J. For. Path. 21:430-438 https://doi.org/10.1111/j.1439-0329.1991.tb00780.x
  10. Kuroda K, Yamada T, Mineo K, Tamura H. 1988. Effects of cavitation on the development of pine wilt disease caused by Bursaphelenchus xylophilus. Annals of the Phytopathological Society of Japan 54:606-615 https://doi.org/10.3186/jjphytopath.54.606
  11. Kwon TS, Lim JH, Sim SJ, Kwon YD, Son SK, Lee KY, Kim YT, Park JW, Shin CH, Ryu SB, Lee CK, Shin SC, Chung YJ, Park YS. 2006. Distribution patterns of Monochamus alternatus and M. saltuarius (Coleoptera: Cerambycidae) in Korea. Jour. Korean For. Soc. 95(5):543-550
  12. Lee YJ. 2002. Dominant height growth and site index curves for pitch-loblolly hybrid pine (Pinus rigida × P. taeda) plantations. Jour. Korean For. Soc. 91(5):661-666
  13. Mamiya Y. 1972a. Pine wood nematode, Bursaphelenchus lignicolus Mamiya and Kiyohara, as a causal agent of pine wilting disease. Rev. Plant Prot. Res. 5:46-60
  14. Mamiya Y. 1972b. Reproduction of pine lethal wilting disease by the inoculation of young trees with Bursaphelenchus lignicolus. Jpn. J. Nematol. 2:40-44
  15. Mamiya Y. 1980. Inoculation of the first year pine (Pinus densiflora) seedlings with the pine wood nematode Bursaphelenchus lignicolus and histopathology of diseased seedlings. J. Jpn. For. Soc. 62:176-183
  16. Mamiya Y. 1983. Pathology of the pine wilt disease caused by Bursaphelenchus xylophilus. Annu. Rev. Phytopathol. 21:201-220 https://doi.org/10.1146/annurev.py.21.090183.001221
  17. Mamiya Y, Enda N. 1972. Transmission of Bursaphelenchus lignicolus (Nematoda: Aphelenchoididae) by Monochamus alternatus (Coleoptera: Cerambycidae). Nematologica 18:159-162 https://doi.org/10.1163/187529272X00395
  18. Mori Y, Miyahara F, Tsutsumi Y, Kondo R. 2008. Relationship between resistance to pine wilt disease and the migration or proliferation of pine wood nematodes. Eur. J. Plant Pathol. 122:529-538 https://doi.org/10.1007/s10658-008-9321-2
  19. Sasaki M, Furukoshi T. 1976. The initial performances of interspecific hybrids, Pinus thunbergii × P. massoniana and P. tabulaeformis. Trans. Meet. Jpn. For. Soc. 87th, pp. 183-184. Tokyo: J. For. Res. pp. 421
  20. Southey JF. 1986. Laboratory methods for work with plant and soil nematodes, 6th edn. HMSO, London
  21. SYSTAT Software Inc. 2004. SYSTAT 11, Statistics II. SYSTAT Software Inc., Richmond, CA
  22. Tamura H, Mineo K, Yamada T. 1987. Blockage of water conduction in Pinus thunbergii inoculated with Bursaphelenchus xylophilus. Jpn. J. Nematol. 17:23-30
  23. Tan JJ, Ye JR. 2003. Research advance in pathogenic mechanism of pine wood nematode disease. Jour. Huazhong Agric. Univ. 22:613-617
  24. Tan JJ, Ye JR, Wu XQ, Zhu YF, Li Y. 2005. A study on disease development and early diagnosis of pine wood nematode, Bursaphelenchus xylophilus, infection of Japanese black pine. Nematol. 7:481-485 https://doi.org/10.1163/156854105774384787
  25. Trudgill DL. 1991. Resistance to and tolerance of plant parasitic nematodes in plants. Annu. Rev. Phytopathol. 29:167-192 https://doi.org/10.1146/annurev.py.29.090191.001123
  26. Wang YY, Li HY, Shu CR, Guo ZH. 2001. A study on difference of pH between healthy and pine wilt diseased woods of several pine species. Acta Phytopathologica Sinica 31:342-348
  27. Wingfield MJ, Bedker PJ, Blanchette RA. 1986. Pathogenicity of Bursaphelenchus xylophilus in pines in Minnesota and Wisconsin. J. Nematol. 18:44-49
  28. Woo KS, Kim YS, Koo YB, Yeo JK, Moon YS. 2007. Variation in susceptibility of pine species seedlings with the pinewood nematode, Bursaphelenchus xylophilus, in greenhouse, Plant Pathol. J. 23:70-75 https://doi.org/10.5423/PPJ.2007.23.2.070
  29. Yamada T. 2006. Biochemical responses in pines infected with Bursaphelenchus xylophilus. J. Jpn. For. Soc. 88:370-382 https://doi.org/10.4005/jjfs.88.370