Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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A portable surface plasmon resonance sensor system for detection of C-reactive protein using SAM with dimer structure

소형 표면 플라즈몬 공명 센서와 이합체 구조를 가진 SAM을 이용한 CRP 검출

  • Sin, Eun-Jung (Department of Biomedical Engineering, Catholic University of Daegu) ;
  • Joung, Eun-Jung (Department of Biomedical Engineering, Catholic University of Daegu) ;
  • Jo, Jin-Hee (Department of Biomedical Engineering, Catholic University of Daegu) ;
  • Hwang, Dong-Hwan (Department of Biomedical Engineering, Catholic University of Daegu) ;
  • Sohn, Young-Soo (Department of Biomedical Engineering, Catholic University of Daegu)
  • 신은정 (대구가톨릭대학교 의공학과) ;
  • 정은정 (대구가톨릭대학교 의공학과) ;
  • 조진희 (대구가톨릭대학교 의공학과) ;
  • 황동환 (대구가톨릭대학교 의공학과) ;
  • 손영수 (대구가톨릭대학교 의공학과)
  • Received : 2010.09.16
  • Accepted : 2010.11.03
  • Published : 2010.11.30
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Abstract

The detection of C-reactive protein(CRP) using self-assembled monolayer(SAM) was investigated by a portable surface plasmon resonance(SPR) sensor system. The CRP is a biomarker for the possible cardiovascular disease. The SAM was formed on gold(Au) surface to anchor the monoclonal antibody of CRP(anti-CRP) for detection of CRP. Sequence injection of the anti-CRP and bovine serum albumin(BSA) into the sensor system has been carried out immobilize the antibody and to prevent non-specific binding. The portable SPR system has two flow channels: one for the sample measurements and the other for the reference. The output SPR signal was increased with the injection of the anti-CRP, BSA and CRP due to binding of the proteins on the sensor chip. The valid output SPR signals was linearly related to the critical range of the CRP concentration. The experimental results showed the feasibility of the portable SPR system with newly developed SAM to diagnose a risk of the future cardiovascular events.

Keywords

References

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