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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Quantitative measurement of salivary testosterone in Korean adults by stable isotope-dilution liquid chromatographyelectrospray-tandem mass spectrometry

  • Lee, Sang-Hoo (Department of Bioanalysis and Mass Spectrometry, Seoul Medical Science Institute & Seoul Clinical Laboratories) ;
  • Kwon, Soon-Ho (Department of Bioanalysis and Mass Spectrometry, Seoul Medical Science Institute & Seoul Clinical Laboratories) ;
  • Shin, Hye-Jin (Department of Bioanalysis and Mass Spectrometry, Seoul Medical Science Institute & Seoul Clinical Laboratories) ;
  • Park, Ji-Myeong (Department of Bioanalysis and Mass Spectrometry, Seoul Medical Science Institute & Seoul Clinical Laboratories) ;
  • Lim, Hwan-Sub (Department of Laboratory Medicine, Kwandong University College of Medicine) ;
  • Lee, Kyoung-Ryul (Department of Bioanalysis and Mass Spectrometry, Seoul Medical Science Institute & Seoul Clinical Laboratories) ;
  • Kim, Young-Jin (Department of Bioanalysis and Mass Spectrometry, Seoul Medical Science Institute & Seoul Clinical Laboratories)
  • Received : 2010.08.02
  • Accepted : 2010.10.14
  • Published : 2010.11.30
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Abstract

Salivary testosterone levels in Korean adults were quantitatively measured for the first time by liquid chromatography-electrospray-tandem mass spectrometry (LC ESI MS/MS). Salivary testosterone was separated on a multiple reaction monitoring (MRM) chromatogram within 7 min. The LC ESI MS/MS assay was validated over the linearity range of 0.01-2.00 ng/ml (r=0.99987) using testosterone-$d_3$ as an internal standard. The lower limit of quantification (LOQ) was 0.01 ng/ml. The intra- and inter-assay precisions were 1.54% to 4.09% and 0.96% to 4.29%, respectively. The mean recovery was 93.32% (range 88.43-98.05%). The validated assay was then applied to measure the salivary testosterone levels of Korean adults. In men, the salivary testosterone level collected between 9：00-11：00 am was approximately 2.8 times higher than that in women (P < 0.0001). Salivary testosterone levels in both sexes negatively correlated with age. The present assay would also be useful in measuring salivary testosterone levels in clinical laboratories.

Keywords

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