Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidative Activities of Water Extracts from Different Parts of Taraxacum officinale

민들레의 부위별 열수 추출물의 항산화 활성

  • Han, Eun-Kyung (Dept. of Food and Nutrition & Korea Nutrition Institute, Hallym University) ;
  • Lee, Ji-Young (Dept. of Food and Nutrition & Korea Nutrition Institute, Hallym University) ;
  • Jung, Eui-Jin (Dept. of Food and Nutrition & Korea Nutrition Institute, Hallym University) ;
  • Jin, Yong-Xie (Division of Functional Food & Nutrition, National Academy of Agricultural Sciences, Rural Development Administration) ;
  • Chung, Cha-Kwon (Dept. of Food and Nutrition & Korea Nutrition Institute, Hallym University)
  • 한은경 (한림대학교 식품영양학과) ;
  • 이지영 (한림대학교 식품영양학과) ;
  • 정의진 (한림대학교 식품영양학과) ;
  • 김영섭 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 정차권 (한림대학교 식품영양학과)
  • Received : 2010.08.04
  • Accepted : 2010.09.27
  • Published : 2010.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to examine antioxidative and physiological activities of water extracts from different parts (flower, leaf, root, and the whole plant) of Taraxacum officinale. The water extracts from different parts were measured to obtain total flavonoids content, total polyphenol content, electron donating ability, SOD-like activity, nitrite-scavenging ability and tyrosinase inhibition effects. Total flavonoids and total polyphenol contents in flower extract were 32.91 mg/g and 49.31 mg/g, respectively, which were much higher than those of any other parts. The electron donating abilities of flower, leaf, the whole plant, and root extracts were 87.07%, 87.66%, 81.06% and 66.20%, respectively at a concentration of 1 mg/mL. The activities increased in a dose-dependent manner. The SOD-like activity of water extracts from different parts showed 9.07~10.97% at a concentration of 1 mg/mL. The nitrite-scavenging abilities of flower and leaf extracts measured at pH 1.2 were 36.34% and 38.16%, respectively at a concentration of 1 mg/mL. Tyrosinase inhibition activity of the leaf extract at a concentration of 1 mg/mL was the highest (34.19%) and that of the whole plant and root extracts was shown to be more than 20%. These results suggest that water extracts from different parts of Taraxacum officinale could be used as an antioxidative functional food source.

민들레 꽃, 잎, 뿌리, 전초의 부위별 항산화성과 생리활성을 탐색하기 위해 열수 추출물에서 총 플라보노이드와 총폴리페놀 함량을 측정하였으며 항산화활성 그리고 tyrosinase 억제활성을 분석하였다. 총 플라보노이드 및 폴리페놀 함량은 다른 부위보다 꽃 추출물에서 32.91 mg/g, 49.31 mg/g으로 가장 높았다. 전자공여능은 1 mg/mL의 농도에서 꽃, 잎 그리고 전초 추출물이 각각 87.07%, 87.66%, 81.06%를, 뿌리 추출물은 66.20% 나타내었으며 농도 의존적으로 활성이 증가되었다. SOD 유사활성능은 1.0 mg/mL의 농도에서 부위별 추출물이 9.07~10.97%를 나타내었다. pH 1.2 조건에서 측정한 아질산염 소거능은 1 mg/mL의 농도에서 꽃과 잎 추출물이 각각 36.34%, 38.16%로 전초와 뿌리보다 높았다. Tyrosinase 저해활성은 1 mg/mL의 농도에서 잎추출물이 34.19%로 가장 높았으며 전초와 뿌리 추출물에서도 20% 이상의 저해활성이 나타났다. 이상의 결과 민들레 꽃, 잎, 뿌리 및 전초 열수 추출물이 우수한 항산화 활성을 지녀 이를 기능성 건강식품의 소재로 활용할 수 있다고 사료된다.

Keywords

References

  1. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. 2007. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39: 44-84. https://doi.org/10.1016/j.biocel.2006.07.001
  2. Byers T, Perry G. 1992. Dietary carotenes, vitamin C and vitamin E as protective antioxidants in human cancers. Ann Rev Nutr 12: 135-159.
  3. Lawrence RA, Burk F. 1976. Glutathione peroxidase activity in selenium deficient rat liver. Biochem Biophys Res Commun 71: 952-958. https://doi.org/10.1016/0006-291X(76)90747-6
  4. Namiki MO. 1990. Antioxidants/antimutagens in food. Crit Rev Food Sci Nutr 29: 273-300. https://doi.org/10.1080/10408399009527528
  5. Lee YS. 2007. Antioxidative and physiological activity of extracts of Angelica dahurica leaves. Korean J Food Preserv 14: 78-86.
  6. Kim TJ. 1994. Our Flower, 100 Species. 9th ed. Hyunamsa, Seoul, Korea. p 2-5.
  7. Lee HH, Kim YS, Park HY. 2007. Plant regeneration via organogenesis from leaf explant culture of Taraxacum coreanum Nakai. Korean J Med Crop Sci 15: 62-66.
  8. Williams CA, Goldstone F, Greenham J. 1996. Flavonoids, cinnamic acids and coumarins from the different tissues and medicinal preparations of Taraxacum officinale. Phytochemistry 42: 121-127. https://doi.org/10.1016/0031-9422(95)00865-9
  9. Katrin S, Carle R, Schieber A. 2006. Taraxacum-A review on its phytochemical and pharmacological profile. J Ethnopharmacol 107: 313-323. https://doi.org/10.1016/j.jep.2006.07.021
  10. Kang MJ, Seo YH, Kim JB, Shin SR, Kim KS. 2000. The chemical composition of Taraxacum officinale consumed in Korea. Korean J Soc Food Sci 16: 182-187.
  11. Jo JW. 1999. Compositae 10. In Encyclopedia of Oriental Medicine. Kyung Hee University Press, Seoul, Korea. p 514.
  12. Kim JK. 1997. Compositae 1. In I llustrated Natural Drugs Encyclopedia. Namsundang, Seoul, Korea. p 77.
  13. Han SH, Hwang JK, Park SN, Lee KH, Ko KI, Kim KS, Kim KH. 2005. Potential effect of solvent fractions of Taraxacum mongolicum H. on protection of gastric mucosa. Korean J Food Sci Technol 37: 84-89.
  14. Hu C, Kitts DD. 2003. Antioxidant, prooxidant, and cytotoxic activities of solvent-fractionated dandelion (Taraxacum officinale) flower extracts in vitro. J Agric Food Chem 51: 301-310. https://doi.org/10.1021/jf0258858
  15. Ho C, Choi EJ, Yoo GS, Kim KM, Ryu SY. 1998. Desacetylmatricarin, an anti-allergic component from Taraxacum platycarpum. Planta Med 64: 577-578. https://doi.org/10.1055/s-2006-957520
  16. Kim KH, Chun HJ, Han YS. 1998. Screening of antimicrobial activity of the dandelion (Taraxacum platycarum) extract. Korean J Soc Food Sci 24: 114-118.
  17. Takasaki M, Konoshima T, Tokuda H, Masuda K, Arai Y, Shiojima K, Ageta H. 1999. Anti carcinogenic activity of Taraxacum plant. I. Biol Pharm Bull 22: 602-605. https://doi.org/10.1248/bpb.22.602
  18. Takasaki M, Konoshima T, Tokuda H, Masuda K, Arai Y, Shiojima K, Ageta H. 1999. Anti carcinogenic activity of Taraxacum plant. II. Biol Pharm Bull 22: 606-610. https://doi.org/10.1248/bpb.22.606
  19. Cho SY, Park YJ, Oh YJ, Jang JY, Park EM, Kim MJ, Kim KS. 2000. Effect of dandelion leaf extracts on lipids metabolism in rats high cholesterol diet. J Korean Soc Food Sci Nutr 29: 676-682.
  20. Tina H, Tanya H. 1993. Medicinaleherbs. A Dorling Kindersley Book, London, UK. p 101-102.
  21. Kang MJ, Kim KS. 2001. Current trends of research and biological activities of dandelion. Food Industry Nutr 6: 60-67.
  22. Zhishen J, Mengcheng T, Jianming W. 1999. Determination of flavonoid contents in mulberry and other scavenging effects on superoxide radicals. Food Chem 64: 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2
  23. Singleton VL, Rossi JA Jr. 1965. Calorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16: 144-158.
  24. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  25. Marklund S, Marklund G. 1975. Involvement of superoxide anion radical in the oxidation of pyrogallol and convenient assay for superoxide dismutase. Eur J Biochem 47: 468-474.
  26. Gray JI, Dugan Jr LR. 1975. Inhibition of N-nitrosamine formation in model food system. J Food Sci 40: 981-984. https://doi.org/10.1111/j.1365-2621.1975.tb02248.x
  27. Yagi A, Kanbara T, Morinobu N. 1987. Inhibition of mushroom tyrosinase by aloe extract. Planta Med 53: 517-519. https://doi.org/10.1055/s-2006-962799
  28. Koh YJ, Cha DS, Choi HD, Park YK, Choi IW. 2008. Hot water extraction optimization of dandelion leaves to increase antioxidant activity. Korean J Food Sci Technol 40: 283-289.
  29. Lee YS, Ahn DS, Joo EY, Kim NW. 2009. Antioxidative activities of Syneilesis palmata extracts. J Korean Soc Food Sci Nutr 38: 1471-1477. https://doi.org/10.3746/jkfn.2009.38.11.1471
  30. Ferreres F, Gomes D, Valentano P, Goncalves R, Pio R, Chagas EA, Seabra RM, Andrade PB. 2009. Improved loquat (Eribotrya japonica Lindl) cultivars: variation of phenolics and antioxidative potential. Food Chem 114: 1019-1027. https://doi.org/10.1016/j.foodchem.2008.10.065
  31. Heo SI, Wang MH. 2008. Antioxidant activity and cytotoxicity effect of extracts from Taraxacum mongolicum H. Korean J Pharmacogn 39: 255-259.
  32. Lim AK, Kim JO, Jung MJ, Jung HK, Hong JH, Kim DI. 2008. Functional biological activity of hot water and ethanol extracts from Taraxaci Herba. J Korean Soc Food Sci Nutr 37: 1231-1237. https://doi.org/10.3746/jkfn.2008.37.10.1231
  33. Park SJ, Song SW, Seong DH, Park DS, Kim SS, Gou J, Ahn JH, Yoon WB, Lee HY. 2009. Biological activities in the extract of fermented Codonopsis lanceolata. J Korean Soc Food Sci Nutr 38: 983-988. https://doi.org/10.3746/jkfn.2009.38.8.983
  34. Cho YJ, Ju IS, Chun SS, An BJ, Kim JH, Kim MY, Kwon OJ. 2008. Screening of biological activities of extracts from Rhododendron mucronulatum Turcz. flowers. J Korean Soc Food Sci Nutr 37: 276-281. https://doi.org/10.3746/jkfn.2008.37.3.276
  35. Kim EY, Baik IH, Kim JH, Kim SR, Rhyu MR. 2004. Screening antioxidant activity of some medicinal plants. Korean J Food Sci Technol 36: 333-338.
  36. Miliauskas G, Venskutonis PR, Been TVA. 2004. Screening of radical scavenging activity of some medicinal and aromatic plants extracts. Food Chem 85: 231-237. https://doi.org/10.1016/j.foodchem.2003.05.007
  37. Lee KD, Chang HK, Kim HK. 1997. Antioxidative and nitrite scavenging activities of edible mushroom. Korean J Food Sci Technol 29: 432-436.
  38. Kang MJ, Shin SR, Kim KS. 2002. Antioxidative and free radical scavenging activity of water extract from dandelion (Taraxacum officinale). Korean J Food Preserv 9: 253-259.
  39. Park CS, Kwon CJ, Choi MA, Park GS, Choi KH. 2002. Antioxidative and nitrite scavenging activities of Mugwort and pine needle extracts. Korean J Food Preserv 9: 248-252.
  40. Kim HK, Kim YE, Do JR, Lee YC, Lee BY. 1995. Antioxidative activity and physiological activity of some Korean medicinal plants. Korean J Food Sci Technol 27: 80-85.
  41. Kitani K, Minami C, Amamoto T, Kanai S, Ivy GO, Carrillo MC. 2002. Pharmacological interventions in aging and age-associated disorders: potentials of propargylamines for human use. Ann N Y Acad Sci 959: 295-307. https://doi.org/10.1111/j.1749-6632.2002.tb02101.x
  42. Lim JD, Yu CY, Kim MJ, Yun SJ, Lee SJ, Kim NY. 2004. Comparison of SOD activity and phenolic compound contents in various Korean medicinal plants. Korean J Med Crop Sci 12: 191-202.
  43. Choi BD, Park CS, Joo EY. 2008. Physiological activities of Korean and Chinese Viola mandsurica extracts. J Korean Soc Food Sci Nutr 39: 1101-1108. https://doi.org/10.3746/jkfn.2008.37.9.1101
  44. Hong TG, Lee YR, Yim MH, Chung NH. 2004. Physiological functionality and nitrite scavenging ability of fermentation extracts from pine needles. Korean J Food Preserv 11: 94-99.
  45. Prota G. 1988. Progress in the chemistry of melanins and related metabolites. Med Res Rev 8: 525-556. https://doi.org/10.1002/med.2610080405
  46. Jung SW, Lee MK, Kim SJ, Han DS. 1995. Screening of tyrosinase inhibitor from plants. Korean J Food Sci Technol 27: 891-896.
  47. An BJ, Lee CE, Son JH, Lee JY, Choi GH, Park TS. 2005. Antioxidant, anticancer and tyrosinase inhibition activities of extracts from Rhododendron mucronulatum T. J Korean Soc Appl Biol Chem 48: 280-284.
  48. Kim YJ, Jeong JA, Kwon SH, Lee CH. 2008. Comparison of biological activities of extracts from different parts and solvent fractions in Cornus kousa Buerg. Korean J Plant Res 21: 28-35.
  49. Im MH, Park YS, Cho CJ, Heo BG. 2008. Assessment of the physiological activities of flower extracts from white lotus. Korean J Community Living Sci 19: 3-10.

Cited by

  1. Antioxidant Activity and Cytotoxicity of Ethanol Extracts from Different Parts of Taraxacum coreanum Nakai cultivated in South Korea vol.28, pp.4, 2015, https://doi.org/10.9799/ksfan.2015.28.4.594
  2. Nutritional Components and Antioxidant Activity of Dry Bitter Melon (Momordica charantia L.) vol.45, pp.4, 2016, https://doi.org/10.3746/jkfn.2016.45.4.518
  3. Antioxidant Activity and Inhibitory Effect of Taraxacum officinale Extracts on Nitric Oxide Production vol.45, pp.2, 2013, https://doi.org/10.9721/KJFST.2013.45.2.206
  4. Comparison of Antioxidant Activities of Water Extract from Dandelion (Taraxacum officinale) Aerial Parts, Roots, and Their Mixtures vol.44, pp.8, 2015, https://doi.org/10.3746/jkfn.2015.44.8.1157
  5. Anti-inflammatory Effect and Antioxidative Activities of Ingredients used in Bibimbab vol.23, pp.2, 2013, https://doi.org/10.5352/JLS.2013.23.2.213
  6. Quality Characteristics of Ground Pork Meat containing Hot Water Extract from Dandelion (Taraxacum officinale)     vol.25, pp.4, 2015, https://doi.org/10.17495/easdl.2015.8.25.4.651
  7. Antioxidative and Anticancer Activities of Water Extracts from Different Parts of Taraxacum coreanum Nakai Cultivated in Korea vol.44, pp.8, 2015, https://doi.org/10.3746/jkfn.2015.44.8.1234
  8. Anti-Inflammatory Activities of Extracts from Fermented Taraxacum platycarpum D. Leaves Using Hericium erinaceum Mycelia vol.45, pp.1, 2016, https://doi.org/10.3746/jkfn.2016.45.1.020
  9. Antioxidative and Antidiabetic Activities of Methanol Extracts from Different Parts of Jerusalem Artichoke (Helianthus tuberosus L.) vol.29, pp.1, 2016, https://doi.org/10.9799/ksfan.2016.29.1.128
  10. Screening of Peruvian Medicinal Plants for Tyrosinase Inhibitory Properties: Identification of Tyrosinase Inhibitors in Hypericum laricifolium Juss vol.22, pp.3, 2017, https://doi.org/10.3390/molecules22030402
  11. Biological Activity of Korean Dandelion (Taraxacum coreanum) Extracts and Preparation of Korean Dandelion Tea by Roasting Time vol.31, pp.5, 2015, https://doi.org/10.9724/kfcs.2015.31.5.581
  12. 랫드에서 thioacetamide의 반복 투여로 유도된 간 손상에 대한 민들레 추출물의 효과 vol.40, pp.2, 2017, https://doi.org/10.7853/kjvs.2017.40.2.107
  13. Persicaria thunbergii Extract as a Physiologically Active Cosmetic Ingredient vol.16, pp.3, 2010, https://doi.org/10.20402/ajbc.2018.0221
  14. 마치현 에탄올 추출물의 항균, 항산화 효과 vol.35, pp.3, 2010, https://doi.org/10.12925/jkocs.2018.35.3.975
  15. Habitat conditions strongly affect macro- and microelement concentrations in Taraxacum microspecies growing on coastal meadows along a soil salinity gradient vol.8, pp.None, 2010, https://doi.org/10.7717/peerj.10233