Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Analyses of Capsaicinoids and Ascorbic Acid in Pepper (Capsicum annum L.) Breeding Lines

고추의 육성계통에 따른 Capsaicinoids와 Ascorbic Acid 분석

  • Jung, Mi-Ri (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Hwang, Young (Korean Food & Culture Division, Rural Development Administration) ;
  • Kim, Hae-Young (Dept. of Food Science and Nutrition, Yong In University) ;
  • Jeong, Heong-Sang (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Park, Ji-Sung (Jeil Seed Company) ;
  • Park, Dong-Bok (Jeil Seed Company) ;
  • Lee, Jun-Soo (Dept. of Food Science and Technology, Chungbuk National University)
  • 정미리 (충북대학교 식품공학과) ;
  • 황영 (농촌진흥청 국립농업과학원 농식품자원부 전통한식과) ;
  • 김혜영 (용인대학교 식품영양학과) ;
  • 정헌상 (충북대학교 식품공학과) ;
  • 박지성 (제일종묘 육종연구소) ;
  • 박동복 (제일종묘 육종연구소) ;
  • 이준수 (충북대학교 식품공학과)
  • Received : 2010.06.14
  • Accepted : 2010.11.08
  • Published : 2010.11.30
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Abstract

Peppers (Capsicum annuum L.) are a rich source of phytochemicals including polyphenolics, flavonoids, capsaicinoids and ascorbic acid. Capsaicinoids are a group of 12 or more related alkaloids responsible for the pungent sensation in the fruits of the genus Capsicum. Ascorbic acid is another functional and nutritional constituent of peppers. In this study, the contents of two major capsaicinoids (capsaicin and dihydrocapsaicin) and ascorbic acid in 131 pepper breeding lines were quantified by HPLC. In 131 pepper breeding lines, capsaicin and dihydrocapsaicin contents were in the range of 0.0 to 219.6 and 0.0 to 110.8 mg/100 g, respectively. The breeding lines with higher capsaicin content contained higher dihydrocapsaicin content as well. Ascorbic acid contents were 264.9 to 1695.5 mg/100 g for the 131 pepper breeding lines. The analytical method validation parameters including accuracy, repeatability, and reproducibility were calculated to ensure the method's validity. This study provides basic information to plant breeders and biotechnologists who are planning to breed genotypes with high content of phytochemicals.

본 연구에서는 130여종의 육성계통 고추의 capsaicinoids와 ascorbic acid 등의 기능성 성분의 함량을 분석함으로써 부가가치 높은 우수 고추 품종 개발을 위한 database를 제공하고자 하였다. Capsaicinoids 및 ascorbic acid 분석을 위해 역상 HPLC를 이용하였다. 연구 결과 capsaicin의 평균함량은 29.0 mg/100 g, dihydrocapsaicin은 15.8 mg/100 g으로 나타났으며 ascorbic acid의 평균 함량은 809.8 mg/100 g이었다. 육성계통 고추 No.2687의 capsaicin 함량은 219.6 mg/100 g, dihydrocapsaicin 함량은 110.8 mg/100 g으로 가장 높게 분석되었으나 ascorbic acid 함량은 264.9 mg/100 g으로 가장 낮게 분석되었다. 또한 육성계통 고추 No. 2524가 ascorbic acid의 함량이 1695.5 mg/100 g으로 가장 높았으나 capsaicinoid 함량은 평균 이하로 낮게 나타났다. 분석방법을 검증하기 위해 재현성, 반복성 및 회수율을 구하였으며 그 결과 재현성과 반복성의 CV는 5% 이하였으며 회수율은 90% 이상으로 우수하였다. 본 연구 결과는 고추의 육성 계통별 기능성 성분의 함량을 분석하고 그 결과를 토대로 우수품종 개발을 위한 기초자료를 제공함에 그 의의가 있다.

Keywords

References

  1. Cho YS, Cho MC, Suh HD. 2000. Current status and projects of national hot pepper industry in Korea. J Korean Capsicum Res Coop 6: 1-27.
  2. Park JB, Lee SM, Kim S. 2000. Capsaicinoids control of red pepper powder by particle size. J Korean Capsicum Res Coop 6: 51-62.
  3. Kang IH. 1983. Hankook Shiksenghwalsa. Samyongsa, Seoul, Korea. p 190.
  4. Park CR, Lee KJ. 1975. A study on the influence of drying methods upon the chemical change in red pepper, part 2, changes of free amino acid, free sugar. Korean J Nutr 8: 27-31.
  5. Kim S, Kim KS, Park JB. 2006. Changes of various chemical components by the difference of the degree of ripening and harvesting factors in two single-harvested peppers (Capsicum annuum L.). Korean J Food Sci Technol 38: 615-620.
  6. Lee SW. 1979. Gas liquid chromatographic studies on sugars and organic acid in different portions of hot pepper fruit (Capsicum annuum L.). Korean J Food Sci Technol 11: 278-282.
  7. Kawaguci Y, Ochi T, Takaishi Y, Kawazoe K, Lee KH. 2004. New sesquiterpenes from Capsicum annuum L. J Nat Prod 67: 1893-1896. https://doi.org/10.1021/np0305472
  8. Davis BH, Mathews S, Kirk JTO. 1970. The nature and biosynthesis of the carotenoids of different colour varieties of Capsicum annuum L. Phytochemistry 9: 797-800. https://doi.org/10.1016/S0031-9422(00)85183-3
  9. Materska M, Piacente S, Stochmal A, Pizza C, Olezek W, Peruka I. 2003. Isolation and structure elucidation of flavonoid and phenolic acid glycosides from pericarp of hot pepper fruit Capsicum annuum L. Phytochemistry 63: 893-898. https://doi.org/10.1016/S0031-9422(03)00282-6
  10. Chiang GH. 1986. HPLC analysis of capsaicins and simultaneous determination of capsaicins and piperine by HPLC-ECD and UV. J Food Sci 51: 499-505. https://doi.org/10.1111/j.1365-2621.1986.tb11165.x
  11. Zhang WY, Po ALW. 1994. The effectiveness of topically applied capsaicin. Eur J Clin Pharmacol 46: 517-522.
  12. Mori A, Lehman S, O'kelly J, Kumagai T, Desmond JC, Pervan M, McBride WH, Kizaki M, Koeffler P. 2006. Capsaicin, a component of red pepper, inhibits the growth of androgen-independent, p53 mutant prostate cancer cells. Cancer Res 66: 3222-3229. https://doi.org/10.1158/0008-5472.CAN-05-0087
  13. Howard LR, Smith RT, Wagner AB, Villalon B, Burns EE, 1994. Provitamin A and ascorbic acid content of fresh pepper cultivars (Capsicum annuum L.) and processed jalapenos. J Food Sci 59: 362-365. https://doi.org/10.1111/j.1365-2621.1994.tb06967.x
  14. Topuz A, Ozdemir F. 2007. Assessment of carotenoids, capsaicinoids and ascorbic composition of some selected pepper cultivars (Capsicum annuum L.) grown in Turkey. J Food Compos Anal 20: 596-602. https://doi.org/10.1016/j.jfca.2007.03.007
  15. Deepa N, Kaur C, George B, Singh B, Kapoor HC. 2007. Antioxidant constituents in some sweet pepper (Capsicum annuum L.) genotypes during maturity. Food Sci Technol 40: 121-129.
  16. Attuquayefio VK, Buckle KA. 1987. Rapid sample preparation method for HPLC analysis of capsaicinoids in capsicum fruits and oleoresins. J Agric Food Chem 35: 777-779. https://doi.org/10.1021/jf00077a032
  17. Wang HF, Tsai YS, Lin ML, Ou AS. 2006. Comparison of bioactive components in GABA tea and green tea produced in Taiwan. Food Chem 96: 648-653. https://doi.org/10.1016/j.foodchem.2005.02.046
  18. Shin HH, Lee SR. 1991. Quality attributes of Korean red pepper according to cultivars and growing areas. Korean J Food Sci Technol 23: 296-300.
  19. Kim S, Park JH, Wang IK. 2002. Quality attributes of various varieties of Korean red pepper powders (Capsicum annuum L.) and color stability during sunlight exposure. J Food Sci 67: 2957-2961. https://doi.org/10.1111/j.1365-2621.2002.tb08845.x
  20. Park JS, Kim MH, Yu RN. 1999. Approximative study of nutrients and intakes determined from capsaicin contents in powdered soups of Korean instant noodles and hot pepper. J Korean Soc Food Sci Nutr 28: 501-504.
  21. Chai JY, Kim MS, Han IK, Lee SY, Yeo IH. 1994. Relationships between the content and sensory evaluation of pungent principles in red pepper. J Korean Soc Anal Sci 7: 541-545.
  22. Choi SH. 2006. Ascorbic acid of Korean pepper by cultivating season, region and cooking method. J East Asian Soc Dietary Life 16: 578-584.
  23. Kye SH, Lee JD, Paik HY. 1993. Analysis of ascorbic acid contents in raw, processed and cooked foods by HPLC. J Korean Home Assoc 31: 201-208.
  24. Cho BC, Park KW, Kang HM, Lee WM, Choe JS. 2004. Correlationship between climatic elements and internal characteristics of red pepper fruit in different growing periods. J Bio-Env Con 13: 67-72.

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