Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Verification of the Suitability of the ATP Luminometer as the Monitoring Tool for Surface Hygiene in Foodservices

급식소 식품접촉표면 위생 모니터링 도구로서의 ATP Luminometer 적합성 확인

  • Kim, Yang-Sook (Dept. of Food and Nutrition, Changwon National University) ;
  • Moon, Hey-Kyung (Dept. of Food and Nutrition, Changwon National University) ;
  • Kang, Seong-Il (3M Korea Ltd., Health Care Business) ;
  • Nam, Eun-Jeong (Dept. of HACCP Support, Korean Health Industry Development Institute)
  • 김양숙 (국립창원대학교 식품영양학과) ;
  • 문혜경 (국립창원대학교 식품영양학과) ;
  • 강성일 (한국 3M 기술연구소) ;
  • 남은정 (한국보건산업진흥원 HACCP 지원단)
  • Received : 2010.07.20
  • Accepted : 2010.09.08
  • Published : 2010.11.30
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Abstract

The purpose of this study was to verify feasibility of using a ATP Luminometer, real-time hygiene monitoring tool for food contact surfaces in foodservices. For this, 54 cutting boards, 70 knives, 21 rubber gloves in 4 institutional foodservices were studied. ATP (RLU: relatively light unit) values by ATP Luminometer were compared with APC (CFU: colony forming unit) of swabbing culture method using aerobic count plates of 3M petrifilm. ATP ranged from 0 RLU/$cm^2$ to 64693 RLU/$cm^2$ on knives, from 0.1 RLU/$cm^2$ to 6743.6 RLU/$cm^2$ on cutting boards and from 31 RLU/$cm^2$ to 465635 RLU/$cm^2$ on the rubber gloves. APC ranged from 0 CFU/$cm^2$ to 166667 CFU/$cm^2$ on knives, from 0 CFU/$cm^2$ to 1000 CFU/$cm^2$ on cutting boards and from 0 CFU/$cm^2$ to 730000 CFU/$cm^2$ on the rubber gloves. To express the degree of association between ATP and APC, a linear regression was performed. There were significant positive correlations found between log RLU and log CFU on the knives (r=0.84, p<0.001), the cutting boards (r=0.79, p<0.001), the rubber gloves (r=0.78, p<0.001). Results of this study showed the possibility that ATP bioluminescence technique can be used as the monitoring tool for surface hygiene in foodservices.

창원시 소재 단체급식소 4개소에서 사용하는 칼($12\;cm^2$), 도마($100\;cm^2$), 고무장갑(손바닥과 손가락 사이)의 표면을 대상으로 ATP Luminometer와 일반세균수용 건조필름을 이용한 미생물 배양법을 함께 적용하여 ATP(RLU)와 APC (CFU)을 측정하였다. 통계분석 결과, 3가지 조리도구 모두에서 ATP(RLU)와 APC(CFU) 간에 유의적인 양의 상관성이 존재하였다. 각 도구별 상관계수는 칼 0.84(p<0.001), 도마 0.79(p<0.001), 고무장갑 0.78(p<0.001)로 나타났다. 본 실은 단체급식 현장에 적합한 ATP Luminometer 가이드라인을 설정하기에 앞서 실시간적인 표면 위생 모니터링 도구로서의 사용 적합성을 살펴 본 예비실험이며, 도출된 회귀식을 이용하여 도구별 ATP(RLU) 가이드라인을 설정하고 검증할 예정이다.

Keywords

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