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Immuno-Activities of Extracts of Tofu Fermented with Pleurotus eryngii Mycelia

큰느타리버섯 균사체로 제조한 발효두부 추출물의 면역 활성

  • Lee, Sang-Won (Dept. of Microbiological Engineering, Jinju National University) ;
  • Kang, Jong-Woo (Dept. of Food and Nutrition, Sunchon National University) ;
  • Kim, Jae-Yong (Dept. of Food Science and Technology, Kyungpook National University) ;
  • Park, Kyung-Wuk (Dept. of Food and Nutrition, Sunchon National University) ;
  • Park, Seok-Kyu (Dept. of Food and Nutrition, Sunchon National University) ;
  • Joo, Ok-Soo (Dept. of Food Science, Jinju National University) ;
  • Yee, Sung-Tae (Dept. of Biology, Sunchon National University) ;
  • Seo, Kwon-Il (Dept. of Food and Nutrition, Sunchon National University)
  • 이상원 (진주산업대학교 미생물공학과) ;
  • 강종우 (순천대학교 식품영양학과) ;
  • 김재용 (경북대학교 식품공학과) ;
  • 박경욱 (순천대학교 식품영양학과) ;
  • 박석규 (순천대학교 식품영양학과) ;
  • 주옥수 (진주산업대학교 식품과학과) ;
  • 이성태 (순천대학교 생물학과) ;
  • 서권일 (순천대학교 식품영양학과)
  • Published : 2010.01.30

Abstract

In order to improve the functional benefits and storage properties of soybean tofu, fermented tofu was developed using Pleurotus eryngii mycelia. The immune activities of water and methanol extracts of the tofu were investigated. The optimal medium for the growth of Pleurotus eryngii mycelia was PD broth medium and the optimal fermentation period for the tofu was 7 days. The water and methanol extracts of the fermented tofu induced the proliferation of spleen cells at above $0.01 {\mu}g/mL$. The water extract increased IL-2, IFN-$\gamma$ production, while the methanol extract increased IFN-$\gamma$ synthesis. The water and methanol extracts of the fermented tofu induced the NO production in RAW264.7 macrophage cells at above $1 {\mu}g/mL$ and above $10 {\mu}g/mL$ concentration, respectively. The extracts also significantly increased the production of IL-6, TNF-$\alpha$, IL-1$\beta$ and GM-CSF in the cells. These results suggest that the tofu fermented with Pleurotus eryngii mycelia could be developed as a functional tofu.

두부의 기능성 및 저장성을 향상시킬 목적으로 큰느타리버섯 균사체를 이용한 발효두부를 제조하여 물과 메탄올로 추출하여 면역세포 활성에 미치는 효과를 조사하였다. 큰느타리버섯 균사체를 배양하기 위한 최적 배지는 PD broth 배지인 것을 확인하였으며, 큰타리버섯 균사체를 이용한 두부의 최적 발효기간은 7일 정도가 적당하였다. 큰느타리버섯 균사체를 이용하여 발효한 두부의 물 및 메탄올추출물은 $0.01 {\mu}g/mL$ 농도 이상에서 비장세포의 증식을 유도하였으며, 이들 추출물은 IL-6, IFN-$\gamma$ 분비를 유도하는 것으로 나타났다. 발효두부 물 추출물은 대조군에 비해 대식세포의 일산화질소 생산을 $1 {\mu}g/mL$ 농도 이상에서 유의적으로 증가시키는 것을 알 수 있었으며, 메탄올 추출물은 $10 {\mu}g/mL$ 농도 이상에서 그 생산을 증가시켰다. 발효두부 추출물들은 대식세포가 분비하는 IL-6, TNF-$\alpha$, IL-1$\beta$ 및 GM-CSF 분비량을 유의하게 증가시켰다. 따라서 큰느타리버섯 균사체로 발효한 두부는 기능성 두부로 개발이 가능하리라 생각된다.

Keywords

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