Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidative Activity of Solvent Extracts from Synurus excelsus and Synurus palmatopinnatifidus

큰수리취 및 국화수리취 용매추출물의 항산화 활성

  • Lee, Kwang-Jae (Gangwon Agricultural Research and Extention Services) ;
  • Yun, In-Ju (Gangwon Agricultural Research and Extention Services) ;
  • Kim, Hee-Yeon (Gangwon Agricultural Research and Extention Services) ;
  • Kim, Kyung-Hee (Gangwon Agricultural Research and Extention Services) ;
  • Kim, Young-Jin (Gangwon Agricultural Research and Extention Services) ;
  • Kim, Dong-Woon (National Institute of Animal Science, RDA) ;
  • Lim, Sang-Hyun (Gangwon Agricultural Research and Extention Services)
  • Received : 2010.09.06
  • Accepted : 2010.10.19
  • Published : 2010.12.31
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Abstract

The total polyphenol contents and antioxidative activities of water and ethanol extracts from Synurus excelsus and Synurus palmatopinnatifidus var. palmatopinnatifidus were determined. Total polyphenol contents of ESE and ESP were 195.7 and 216.2 mg/g, and were higher than WSE (74.7 mg/g) and WSP (77.4 mg/g). The total flavonoid contents were also higher in ESE (176.6 mg/g) and ESP (148.8 mg/g) than WSE and WSP. DPPH radical scavenging activities of ESE and ESP (73.1 and 73.4%) were higher than WSE and WSP as like total polyphenol and flavonoid contents. Although the DPPH radical scavenging activities of the extracts were proportional to total polyphenol contents, there are no differences between water extracts and ethanol extracts of Synurus excelsus and Synurus palmatopinnatifidus on ABTS radical scavenging activities. Moreover, all the solvent extracts (ESE, ESP, WSE and WSP) had no SOD-like activity. These results suggest that consideration for adoption of method is necessary to evaluate antioxidative activity of extracts from plants including vegetables.

큰수리취와 국화수리취 등 국내 자생하는 수리취 2종의 폴리페놀 및 항산화 활성을 분석한 결과, 추출수율은 두 종간 차이가 없었으며, 총 폴리페놀 함량은 큰수리취와 국화수리취 에탄올 추출물이 195.7 및 216.2 mg/g으로 두 종 모두 물 추출물에 비해 더 높은 것으로 분석되었다. 또한, 총 플라보노이드 함량도 에탄올 추출물이 큰수리취와 국화수리취 모두 물 추출물보다 더 높았으며, 총 폴리페놀은 국화수리취 에탄올 추출물에 가장 많이 함유되어 있는 반면 총 플라보노이드 함량은 큰수리취 에탄올 추출물에서 가장 높았다. 수리취의 항산화 활성 실험 결과, DPPH radical 소거활성은 1mg/mL의 농도에서 큰수리취 및 국화수리취 에탄올 추출물이 각각 73.1% 및 73.4%로 대조물질로 사용한 BHT(81.2%)와 유의적인 차이를 보이지 않을 정도로 우수하였으나, ABTS radical 소거활성은 용매별, 종별 추출물 간에 차이가 없었고 SOD 유사활성은 거의 나타나지 않아 실험방법에 따라 항산화 활성의 차이가 크게 나타났다. 식물 추출물의 정확한 항산화 활성 측정을 위해서는 추출물에 따라 측정방법의 선택에 대한 고려가 필요하며 수리취 역시 추출물에 함유되어 있는 페놀성 물질의 특성 및 항산화 기전에 대한 추가적인 연구가 필요할 것으로 판단된다.

Keywords

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