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Enhancement of Antioxidant Activities of Codonopsis lanceolata and Fermented Codonopsis lanceolata by Ultra High Pressure Extraction

초고압 추출 처리에 의한 더덕 및 발효더덕의 항산화 증진

  • Park, Sung-Jin (Dept. of Tourism Food Service Cuisine, Hallym College) ;
  • Park, Dong-Sik (Funtional Food & Nutrition Division, Rural Development Administration) ;
  • Lee, Su-Bock (Dept. of Food Science and Biotechnology, School of Biotechnology) ;
  • He, Xin-Long (Dept. of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University) ;
  • Ahn, Ju-Hee (Dept. of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University) ;
  • Yoon, Won-Byung (Dept. of Food Science and Biotechnology, School of Biotechnology) ;
  • Lee, Hyeon-Yong (Dept. of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University)
  • 박성진 (한림성심대학 관광외식조리과) ;
  • 박동식 (농촌진흥청 기능성식품과) ;
  • 이수복 (강원대학교 BT특성화학부대학 식품생명공학과) ;
  • 허신용 (강원대학교 BT특성화학부대학 생물소재공학과) ;
  • 안주희 (강원대학교 BT특성화학부대학 생물소재공학과) ;
  • 윤원병 (강원대학교 BT특성화학부대학 식품생명공학과) ;
  • 이현용 (강원대학교 BT특성화학부대학 생물소재공학과)
  • Received : 2010.09.01
  • Accepted : 2010.11.17
  • Published : 2010.12.31

Abstract

This study was designed to evaluate antioxidant activity of low-quality Codonopsis lanceolata treated by fermentation process followed by hydraulic high pressure extraction. C. lanceolata was subjected to 5,000 bar for 30 min at $25^{\circ}C$. The highest phenolics content was observed in the combined treatment of fermentation and high pressure extraction (35.11 mg/100 g), followed by high pressure extraction alone (14.78 mg/100 g) and conventional extraction (14.56 mg/100 g). The content of flavonoids followed the similar pattern as that of total phenolics, showing 280.86 mg/100 g of C. lanceolata treated by the combined fermentation with high pressure extraction whereas 193.05 mg/100 g of C. lanceolata treated by the conventional extraction. The DPPH scavenging activity was 69.32% at 0.6 mg/mL of C. lanceolata treated by the combined process, while the DPPH scavenging activities of C. lanceolata treated by high pressure extraction alone and the conventional extraction were 60.35% and 30.92%, respectively. The highest reducing power of C. lanceolata extract (1.0 mg/mL) was observed at the combined treatment (0.926), followed by high pressure extraction alone (0.881) and the conventional extraction (0.733). The combination of fermentation with high pressure extraction significantly increased the contents of phenolics and flavonoids and also enhanced the antioxidant activity. Therefore, the combined application of fermentation and high pressure extraction can be an alternative technique for the extraction of C. lanceolata. These results provide useful information for enhancing biological properties of low-quality C. lanceolata.

본 연구에서는 초고압 추출공정을 이용하여 전통적인 기존 추출공정과 비교함으로써 초고압 추출공정에 의한 등급 외 더덕 및 발효더덕의 항산화 활성의 증진을 확인하고자 연구를 수행하였다. 총 페놀화합물 함량은 초고압 발효더덕 추출물이 35.11 mg/100 g으로 초고압 더덕 추출물 14.78 mg/100 g, 일반더덕 추출물 14.56 mg/100 g에 비해 총 페놀화합물 함량이 약 2배 이상 높은 수치를 나타내었다. 또한, 플라보노이드 함량은 페놀화합물과 유사한 경향으로 초고압 발효더덕 추출물이 280.86 mg/100 g으로 초고압 더덕 추출물의 198.35 mg/100 g과 일반더덕 추출물 193.05 mg/100 g에 비해 약 1.4배 정도 높은 함량을 나타내었다. 항산화도는 초고압 발효더덕 추출물의 경우 0.6 mg/mL에서 약 70%의 항산화도를 나타내었으며, 이 값은 초고압 더덕 추출물 60.35%와 일반더덕 추출물 30.92%보다 높은 항산화도를 나타내었다. 초고압 발효더덕 추출물의 환원력은 최종농도 1.0 mg/mL에서 일반더덕의 경우 0.733, 초고압 더덕 추출물의 경우 0.881, 초고압 발효더덕 추출물의 경우 0.926 으로서 초고압 발효더덕 추출물의 환원력이 다른 추출물보다 약 0.193까지 높은 결과를 나타내었다. 따라서 초고압 추출공정이 등급 외 더덕 및 발효더덕의 기능성을 증가시킬 수 있으며, 초고압 추출공정의 최적화를 통한 활성물질의 추출 극대화를 통해 높은 경제적 가치를 부여해 줄 수 있을 것으로 사료된다.

Keywords

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