Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Virulence of Ornithobacterium rhinotracheale Isolates for Embryonated SPF Eggs and Broilers

국내에서 분리한 Ornithobacterium rhinotracheale 균의 종란과 육계에서의 병원성

  • Kwon, Yong-Kuk (National Veterinary Research & Quarantine Service) ;
  • Jeon, Woo-Jin (National Veterinary Research & Quarantine Service) ;
  • Kang, Min-Soo (National Veterinary Research & Quarantine Service) ;
  • Oh, Jae-Young (National Veterinary Research & Quarantine Service) ;
  • An, Byung-Ki (National Veterinary Research & Quarantine Service) ;
  • Song, Eun-A (National Veterinary Research & Quarantine Service) ;
  • Kwon, Jun-Hun (National Veterinary Research & Quarantine Service) ;
  • Lee, Cheong-San (Chungbuk Livestock and Veterinary Research Institute) ;
  • Kim, Jae-Hong (College of Veterinary Medicine, Seoul National University)
  • 권용국 (국립수의과학검역원 조류질병과) ;
  • 전우진 (국립수의과학검역원 조류질병과) ;
  • 강민수 (국립수의과학검역원 조류질병과) ;
  • 오재영 (국립수의과학검역원 조류질병과) ;
  • 안병기 (국립수의과학검역원 조류질병과) ;
  • 송은아 (국립수의과학검역원 조류질병과) ;
  • 권준헌 (국립수의과학검역원 조류질병과) ;
  • 이청산 (충북축산위생연구소) ;
  • 김재홍 (서울대학교 수의과대학)
  • Received : 2010.03.19
  • Accepted : 2010.05.31
  • Published : 2010.06.30
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Abstract

Field strains of Ornithobacterium rhinotracheale (OR) were tested on their virulence in specific pathogen free (SPF) embryonated chicken eggs and 3-week-old broilers. When infected with three different OR isolates (OR-161, OR-240 and OR-295) through yolk sac infection route, all strains appeared to be highly pathogenic with responsible mortality 66% and 100% within 12 days post infection (DPI). To test the virulence of OR in the commercial broilers, 3 week-old broilers were grouped depends on the inoculation route of OR isolate (OR-295) through five different infection routes; group 1 (IT: intratracheal), group 2 (IM: intramuscular), group 3 (IV: intravenous), group 4 (aerosol) and group 5 [Mixed: NDV (LaSota)+OR aerosol]. Within 5 to 7 days after inoculation, only broilers given NDV+OR were slightly depressed and coughing, and had mild facial redness. Grossly, foamy and yellow-white yogurt like exudate in the air sacs, predominantly in the abdominal air sacs was present. In histology, infiltration of the air sac epithelium and lamina propria by macrophage and polymorphonuclear granulocytes was seen with cell debris and inflammatory cells, correlated with the presence of OR antigen, as demonstrated by immunohistochemistry. Field strains of OR were able to induce high mortality in the embryonated chicken eggs, whereas broilers were less susceptible to OR infection. Interestingly, in the absence of NDV infection, the four groups of OR single infection only different route showed minimal and temporary microscopic air sac lesions. Thus, Newcastle disease virus (LaSota strain) showed triggering effects on the OR infection in chickens.

본 시험은 국내에서 분리한 Ornithobacterium rhinotracheale(OR) 병원성 세균이 부화중인 종란과 일반 육계에서 얼마만큼 병원성을 보이는지 알아보기 위하여 수행하였다. 첫 번째 9일령 부화란의 yolk sac에 국내에서 분리한 3종의 OR strain을 접종하여 12일동안 관찰한 결과, 66% 이상의 폐사율이 관찰되어 높은 병원성이 인정되었다. 두 번째로 3주령 일반 육계를 대상으로 5가지 다른 공격접종법[Intratracheal, Intravenous, Intramuscular, Aerosol, Newcastle Disease Virus(NDV)와 혼합 Aerosol]으로 접종한 다음 병원성을 관찰하였다. NDV와 OR균을 동시에 분무 접종한 계군에서만 특이적인 임상증상인 침울, 기침, 안면 종대와 함께 부검시 치즈양 또는 요구르트와 비슷한 염증성 삼출물이 기낭에 관찰되었다. 조직학적으로도 기낭상피세포의 변성과 탈락, 대식세포와 다형태성 관립구의 침윤, 부종 등의 기낭염이 확인되었으며, 이들 기낭을 이용한 면역 조직 화학적 염색법을 적용한 결과 다량의 OR균의 항원이 검출되었다. 그러나 OR균만 단독 처리한 닭에서는 일시적이고 경미한 조직학적 병변만이 관찰되었다. 결론적으로 NDV가 OR균의 감염에 따른 임상 증상과 병리조직학적 병변 유발에 주요한 역할을 하는 것으로 판단된다.

Keywords

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