Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Evaluation of Efficacy of PoulShot® MG-F Vaccine against Mycoplasma gallisepticum Infection in the Layer Farms

PoulShot® MG-F 백신의 마이코플라즈마 감염증에 대한 산란계 농장에서의 야외 효능 평가

  • Jeon, Eun-Ok (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Woo, Chang-Gok (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Won, Ho-Keun (Department of QA/R&D, Choongang Vaccine Laboratory) ;
  • Mo, In-Pil (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • Received : 2010.05.27
  • Accepted : 2010.06.14
  • Published : 2010.06.30
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Abstract

Mycoplasma gallisepticum (MG) infection results in severe economic loss in the poultry industry. In the previous reports, F strain, one of the MG live vaccine strains, could protect the bird from field infection of MG strains. In this study, efficacy of PoulShot$^{(R)}$ MG-F vaccine againset mycoplasma gallisepticum infection was evaluated for filed application in commercial layers. Commercial layers from two different farms received with PoulShot$^{(R)}$ MG-F, MG-F live vaccine at 9~14 weeks of age. Serological immune response to MG vaccine, the persistency of MG vaccine strain in the upper respiratory tracts and egg production rate were evaluated in the vaccinated, contacted or nonvaccinated flocks. The serological response was first detected at 3 weeks after vaccination (WAV) and persisted for 31 WAV. The MG vaccine strains were also persisted for 31 WAV based on the reisolation and PCR detection. There was no difference between the vaccinated or non-vaccinated flocks in the egg production rate but in the abnormality rate of eggs. Based on the above results, we suggested that the PoulShot$^{(R)}$, MG-F live vaccine was fully immunogenic and had characteristics of long persistence in the upper respiratory trachea which will reduce economic loss caused by MG infection in the layer farms.

본 연구에서는 Mycoplasma gallisepticum F 주 생독 백신(PoulShot$^{(R)}$ MG-F)의 안전성과 효능을 평가하였다. 충청북도 진천과 경기도 안성 지역의 산란계 농장을 선정하여, 백신과 야외주 공격 접종에 따른 혈청 역가 변화, 상부 호흡기에서의 마이코플라즈마균 재분리, 조직학적 병변과 백신 접종군 및 백신 미접종군 간의 산란율 및 오파란율의 차이를 농장별로 평가하였다. 백신 접종에 의한 혈청 역가 변화는 백신 접종 후 3주부터 확인되었으며, 농장에 따라 접종 후 23주에서 31주까지 지속됨으로써 백신 항체가 오랫동안 유지되는 것을 확인할 수 있었다. 또한 상부 호흡기에서 MG-F 재분리 및 PCR에 의한 유전자 검출도 백신 접종 후 31주까지 양성이었다. 이러한 항체 및 항원의 지속적인 검출은 상부 호흡기에 MG-F 백신주의 집락 형성이 오랫동안 지속된다는 것을 의미하는 것이다. 동일한 방법으로 백신 접종군에 대한 야외주 공격 접종 후 상부 호흡기에서의 백신주 집락 형성을 분석한 결과, 공격 접종 후 3주까지 백신주의 집락 형성율이 야외주보다 동등하거나 높은 것으로 확인됨으로써 야외주 공격에 대한 백신주의 방어력이 입증되었다. MG-F의 안전성과 생산성 측면에서의 효능을 야외 농장에서 검증하기 위하여 두 실험 농장에서 백신 접종군과 백신 미접종군간의 산란율 및 오파란율을 비교하였다. 그 결과, MG-F 접종에 따른 임상적 부작용과 산란율 하락은 발견되지 않았으며, 오히려 백신 접종군의 오파란율이 백신 미접종군보다 평균 1~3% 낮은 것으로 분석됨으로써 백신 접종에 의한 난질 개선 효과가 있음이 확인되었다. 따라서, PoulShot$^{(R)}$ MG-F 생균백신을 산란계에 접종하였을 때 임상적으로 안전하였으며, 오랜 기간 야외 감염에 방어할 수 있는 항체 형성과 상부 호흡기에서의 지속성이 확인됨으로써 마이코플라즈마 야외 감염을 효율적으로 방어할 수 있을 것으로 판단된다.

Keywords

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