Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Distribution of Telomeric DNA in Korean Native Chicken Chromosomes

한국 재래닭 염색체의 텔로미어 분포 양상

  • Sohn, Sea-Hwan (Department of Animal Science & Biotechnology, Jinju National University) ;
  • Cho, Eun-Jeong (Department of Animal Science & Biotechnology, Jinju National University)
  • 손시환 (진주산업대학교 동물생명과학과) ;
  • 조은정 (진주산업대학교 동물생명과학과)
  • Received : 2010.08.13
  • Accepted : 2010.08.31
  • Published : 2010.09.30
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Abstract

Telomeres are nucleoprotein structures at the ends of chromosomes consisting of DNA sequences arranged in tandemly repeated units $(TTAGGG)_n$. However, this hexamers can also occur at non-telomeric sites in some avians and vertbrate. This study was carried out to present the distribution of telomeric DNA sequences in Korean Native Chicken chromosomes. The fluorescence in situ hybridization technique using a telomeric DNA probe was performed to the metaphase spreads of chicken early embryonic cells. Telomeric DNA signals were detected at the ends of chromosomes including macrochromosomes and microchromosomes. In chicken, surprisingly, chromosome 1 showed very distinct interstitial telomeric DNA hybridization patterns which located two interstitial sites in the p-arm at 1p11 and 1p23, and one in the q-arm at 1q32. In chromosome number 2 and 3 also displayed interstitial telomeric signals (ITS) in the long arms at 2q24 and 3q32, respectively. The pattern of telomeric DNA distribution in Korean Native Chicken chromosomes was in agreement with a previously reported in Gallus domesticus. The relative amount of telomeric DNA sequences in each macrochromosomes ranged from 4.6% to 16.3%. Distribution of telomeric DNAs at the end of p-arm was much more than that of q-arm in almost chicken chromosomes. The distribution of ITS in chicken chromsomes implicate to tandem chromosome fusions that might have occurred during the process of karyotype evolution.

텔로미어(telomere)는 염색체 양 말단에 위치하는 DNA와 단백질의 복합체로서 (TTAGGG)n의 단순 반복 염기 서열로 이루어져 있다. 그러나 일부 조류 및 척추동물의 경우 염색체의 양 말단 부위외 간질적 위치에도 telomeric DNA sequence가 분포한다. 본 연구는 닭 염색체에 있어 telomeric DNA의 분포 양상을 제시하고자 한국 재래닭의 초기 배아로부터 염색체 표본을 제작하고, telomeric DNA probe를 이용한 FISH를 수행하여 염색체 상 텔로미어의 분포 양상을 분석하였다. 분석 결과, 닭의 모든 염색체 양 말단부에 텔로미어가 분포하는 것으로 나타났으며, 더불어 대형 염색체 중 1번의 1q32, 1p11, 1p23 위치와 2번 염색체의 2q24 및 3번 염색체 3q32에 interstitial telomeric signal(ITS)이 존재하는 것이 확인되었다. 이러한 한국 재래닭 염색체의 텔로미어 분포 양상은 이전 Gallus domesticus에서 발표한 분포 양상과 거의 일치한 것으로 나타났다. 한국 재래닭의 각 염색체별 텔로미어 함유율은 4.6~16.3% 정도로 분석되었으며, 거의 대부분의 염색체에서 단완 말단부의 telomeric DNA의 함량이 장완 말단부보다 높은 것으로 나타났다. 닭 염색체에서 ITS의 존재와 분포 양상은 핵형학적으로 진화 과정 중 염색체 간의 융합에 의해 신생 염색체가 형성되었을 가능성을 시사한다.

Keywords

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