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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Epigenetic modification of retinoic acid-treated human embryonic stem cells

  • Cheong, Hyun-Sub (Department of Genetic Epidemiology, SNP Genetics, Inc.) ;
  • Lee, Han-Chul (Medical Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Byung-Lae (Department of Genetic Epidemiology, SNP Genetics, Inc.) ;
  • Kim, Hye-Min (Department of Biological Sciences and Center for Stem Cell Differentiation, Korean Advanced Institute of Science and Technology) ;
  • Jang, Mi-Jin (Department of Biological Sciences and Center for Stem Cell Differentiation, Korean Advanced Institute of Science and Technology) ;
  • Han, Yong-Mahn (Department of Biological Sciences and Center for Stem Cell Differentiation, Korean Advanced Institute of Science and Technology) ;
  • Kim, Seun-Young (Medical Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Yong-Sung (Medical Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Shin, Hyoung-Doo (Department of Genetic Epidemiology, SNP Genetics, Inc.)
  • Received : 2010.06.18
  • Accepted : 2010.10.25
  • Published : 2010.12.31
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Abstract

Epigenetic modification of the genome through DNA methylation is the key to maintaining the differentiated state of human embryonic stem cells (hESCs), and it must be reset during differentiation by retinoic acid (RA) treatment. A genome-wide methylation/gene expression assay was performed in order to identify epigenetic modifications of RA-treated hESCs. Between undifferentiated and RA-treated hESCs, 166 differentially methylated CpG sites and 2,013 differentially expressed genes were discovered. Combined analysis of methylation and expression data revealed that 19 genes (STAP2, VAMP8, C10orf26, WFIKKN1, ELF3, C1QTNF6, C10orf10, MRGPRF, ARSE, LSAMP, CENTD3, LDB2, POU5F1, GSPT2, THY1, ZNF574, MSX1, SCMH1, and RARB) were highly correlated with each other. The results provided in this study will facilitate future investigations into the interplay between DNA methylation and gene expression through further functional and biological studies.

Keywords

References

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