한국어병학회지 (Journal of fish pathology)

  • 제23권2호
  • /
  • Pages.145-153
  • /
  • 2010
  • /
  • 1226-0819(pISSN)
  • /
  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
권호 표지

PCR 기법을 이용한 2009년 우리나라 서해안과 남해안 바지락, Ruditapes philippinarum의 Perkinsus olseni 감염에 관한 보고

Survey of Perkinsus olseni infection in Manila clam, Ruditapes philippinarum in 2009 on the west and south coast of Korea using PCR technique

  • 이남실 (국립수산과학원 병리연구과) ;
  • 황지연 (국립수산과학원 병리연구과) ;
  • 최동림 (국립수산과학원 병리연구과) ;
  • 박명애 (국립수산과학원 병리연구과)
  • Lee, Nam-Sil (Division of Pathology, National Fisheries Research and Development Institute) ;
  • Hwang, Jee-Youn (Division of Pathology, National Fisheries Research and Development Institute) ;
  • Choi, Dong-Lim (Division of Pathology, National Fisheries Research and Development Institute) ;
  • Park, Myoung-Ae (Division of Pathology, National Fisheries Research and Development Institute)
  • 투고 : 2010.07.05
  • 심사 : 2010.08.23
  • 발행 : 2010.08.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

내용은 2009년 하반기, 우리나라 남해안(고흥, 나로도)과 서해안(선재도, 파도리)의 네 지점에서 시료채취 한 바지락에서의 Perkinsus olseni 감염에 대한 모니터링 결과로, 이전에 국제수역사무국(OIE)의 manual에서 지정하던 P. olseni에 대하여 특이적인 primer set으로 사용한 PCR 분석 결과와 2009년에 새로이 지정하는 P. olseni에 대하여 특이적인 primer set을 사용한 분석 결과를 비교하고, 결과를 통한 P. olseni의 검출율을 조사하였다. 특히 2009년에 새로이 지정된 PolsITS-140F와 PolsITS-600R의 primer set을 사용한 결과는 신속한 검출여부 분석에 적합한 것으로 생각되었다. P. olseni의 검출경향은 파도리에서 가장 낮았으며, 고흥에서 가장 높았다. 파도리의 7월, 8월 그리고 12월의 결과를 제외하고는 전 지점에서 7월에서 12월까지 지속적으로 높은 검출율을 나타내었다. PolsITS-140F와 PolsITS-600R의 primer set을 이용한 PCR 산물의 염기서열을 분석한 결과, 선재도의 결과에서 두 군데의 염기차이를 나타내는 것 이외에는 모두 같은 것으로 확인되었다.

Prevalence of a protozoan parasite Perkinsus olseni in Manila clam Ruditapes philippinarum was surveyed from July to December 2009 on the west and south coast of Korea. P. olseni infection was diagnosed using two primer sets, P.olseni NTS Forward/P.olseni NTS Reverse set and PolsITS-140F/PolsITS-600R set in polymerase chain reaction(PCR). The results using PolsITS-140F and PolsITS-600R primer set was retained up to 60% at all stations from July to December, except for Padori. Especially, Goheung showed 100% prevalence from October to December. The results about comparison of the 4 station's DNA sequences which were analyzed from PCR products(457bp) using PolsITS-140F and PolsITS-600R primer set, there were only 2base differences at Sunjedo.

키워드

참고문헌

  1. Azevedo, C.: Fine structure of Perkinsus atlanticus n. sp. (apicomplexa, Perkinsea) parasite of the clam Ruditapes decussatus from Portugal. J. Parasitol., 75:627-635, 1989.
  2. Blackbourn, J., Bower, S.M. and Meyer, G.R.: Perkinsus qugwadi sp. nov.(incertace sedis), a pathogenic protozoan parasite of Japanese scallops, Patinopecten yessoensis, cultured in British Columbia, Canada. Can. J. Zool., 7:942-953, 1998.
  3. Burreson, E.M. and Calvo, L.M.R.: Epizootiology of Perkinsus marunus disease of oysters in Chesapeake Bay, with emphasis on data since 1985. J. Shellfish Res., 15:17-34, 1996.
  4. Casas, S.M., Grau, A., Kimberly, S.R., Apalupakul, K., Azevedo, C. and Villalba A.: Perkinsus mediterraneus n. sp., a protistan parasite of the European flat oyster Ostrea edulis from the Balearic Islands, Mediterranean Sea, Dis. Aquat. Org., 58:231-234, 2004. https://doi.org/10.3354/dao058231
  5. Choi, K.S. and Park, K.I.: Review on the Protozoan Parasite Perkinsus olsei(Lester and Davis 1981) Infection in Asian Water. Coastal Environmental and Ecosystem Issues of the East China Sea, Eds., A. Ishimatsu and H.-J. Lie, pp.269-281. 2010.
  6. Choi, K.S., Park K.I., Cho M. and Soudant P.: Diagnosis, Pathology, and Taxonomy of Perkinsus sp. Isolated from the Manila clam Ruditapes philippinarum in Korea. J. Aquaculture, 18:207-214, 2005.
  7. Coss, C.A., Robledo, J.A.F., Ruita, G.M. and Vasta, G.R.: Description of Perkinsus andrewsi n. sp. isolated from the Balthic clam(Macoma balthica) by characterization of the ribosomal RNA locus, and development of a species-specific PCR-based diagnostic assay. J. Eukaryot. Microbiol., 48:52-61, 2001. https://doi.org/10.1111/j.1550-7408.2001.tb00415.x
  8. Cremonte, F., Balseiro, P. and Figueras, A.: Occurrence of Perkinsus olseni(Protozoa:Apicomplexa) and other parasites in the venerid commercial clam Pitar rostrata from Uruguay, southwestern Atlantic coast. Dis. Aquat. Org., 64:85-90, 2005. https://doi.org/10.3354/dao064085
  9. Dungan, C.F. and Reece K.S.: In vitro propagatio of two Perkinsus spp. Parasites from Japanese Manila clams Venerupis philippinarum and Description of Pekinsus honshuensis n. sp. J. Eukaryot. Microbiol. 53: 316-326, 2006 https://doi.org/10.1111/j.1550-7408.2006.00120.x
  10. Goggin, D.L. and Lester, R.J.G.: Perkinsus, a protistan parasite of abalone in Australia: a review. Aust. J. Mar, Freshwater Res., 46:639-646, 1995. https://doi.org/10.1071/MF9950639
  11. Hamaguchi, M., Suzuki, N., Usuk,i H. and Ishioka, H.: Perkinsus protozoan infection in shor-necked clam tapes(=Ruditapesphillippinarum) in Japan. Fish Pathol., 33:73-480, 1998. https://doi.org/10.3147/jsfp.33.73
  12. La Peyre, M., Casas, S. and La Peyre, J.: Salinity effects on viability, metabolic activity and proliferation of three Perkinsus species. Dis. Aquat. Org.,71:59-74, 2006. https://doi.org/10.3354/dao071059
  13. Lester, R.J.G. and Davis, G.H.G.: A new Perkinsus species(Apicomplexa, Perkinsea) from the abalone, Halitis ruber. J. Invert. Pathol. 37:181-187, 1981. https://doi.org/10.1016/0022-2011(81)90073-2
  14. Mackin, J.G., Owen, H.M. and Collier, A.: Preliminary note on the occurrence of a new protistan parasite, Dermocystidium marinum n. sp. in Crassostrea nirginica(Gmelin). Science, 111:328-329, 1950. https://doi.org/10.1126/science.111.2883.328
  15. Marsh, A.G., Gauthier, J.D. and Vasta, G.R.: A seniquantitative PCR assay for assessing Perkinsus marinus infections in the eastern oyster, Crassostrea virginica. J. Parasitol., 81:577-583, 1995. https://doi.org/10.2307/3283856
  16. McLayghlin, S.M., Tall, B.D., Shaheen, A., Elsayed, E.E. and Faisal, M:,Zoosporulation of a new Perkinsus species isolated from the gills of the softshell clam Mya arenaria. Parasite, 7:115-122, 2000. https://doi.org/10.1051/parasite/2000072115
  17. Moss, J.A.: Characterization of exotic pathogens associated with the suminoe oyster, Crassostrea ariakensis. Ph.D. Sissertaion. Virginia Institute of Marine Science. College of William and Mary, Gloucester Point, Virginia USA. p.230, 2007.
  18. Moss, J.A., Xiao, J., Dungan, C.F. and Reece, K.S.: Description of Perkinsus beihaiensis n. sp., a new Perkinsus sp. parasite in oysters of southern China. J. Eukayot. Microbiol. 55:117-130, 2008. https://doi.org/10.1111/j.1550-7408.2008.00314.x
  19. Murell, A., Kleenan, S.N., Barker, S.C. and Lester, R.J.G.: Synonymy of Perkinsus olseni Lester & Davis, 1981 and Perinsus atlanticus Azevedo, 1989 and an update on the phylogenetic position of the genus Perkinsus. Bull. Eur. Assoc. Fish Pathol., 22:258-265, 2002.
  20. Noren, F., Moestrup O. and Rehnstam-Holm A.-S.:Parivilucifera infectans Noren et Moestrup gen. et sp. nov.(Perkinsozoa phylum nov.): a parasitic flagellate capable of killing toxic microalgae. Eur. J. Protistol., 35, 233-254,1999. https://doi.org/10.1016/S0932-4739(99)80001-7
  21. OIE: Manual of Diagnostic Tests for Aquatic Animals, World organization for animal health, Paris, 2009.
  22. Park, K.-I., Ngo, T.T.T., Choi, S.-D., Cho, M. and Choi, K.-S.: Occurrence of Perkinsus olseni in the Venus clam Protothaca jedoensis in Korean waters. J. Inv. Pathol. 93:81-87, 2006. https://doi.org/10.1016/j.jip.2006.04.007
  23. Park, K.-I. and Choi, K.-S.: Spatial distribution of the protozoan parasite Pekinsus sp. found in Manila clams, Ruditapes phillippinarum, in Korea. Aquaculture, 203:9-22, 2001. https://doi.org/10.1016/S0044-8486(01)00619-6
  24. Park, K.I., Park, J.K., Lee, J. and Choi, K.S.: Use of molecular markers for species identification of Korean Perkinsus sp. isolated from Manilaclam Ruditapes philippinarum. Dis. Aquat. Org., 66:255-263, 2005. https://doi.org/10.3354/dao066255
  25. Robledo J.A.F., Coss C.A. and Vasta G.R.: Characterization of the ribosomal RNA locus of Perkinsus atlanticusand development of a polymerase chain reaction-based diagnostic assay. J. Parastol., 86:972-978, 2000. https://doi.org/10.1645/0022-3395(2000)086[0972:COTRRL]2.0.CO;2
  26. Uddin, M.J., Yang, H.S., Choi, K.S., Kim, H.J., Hong, J.S. and Cho, M.: Seasonal Changed in Perkinsus olseni Infection and Gametogenesis in Manil Clam, Ruditapes philippinarum, From Seonjaedo Island in Incheon, off the West Coast of Korea. J. World Aquaculture society, 41:93-101, 2010. https://doi.org/10.1111/j.1749-7345.2009.00337.x
  27. Villalba, A., Casas, S.M., Lopez, C. and Carballal, M.J.: Study of pekinsosis in the carpet shell clam Tapes decussatus in Galicia(NW Spain) II. Temporal pattern of disease dynamics and association with clam mortality. Dis. Aquat. Organ., 65:257-267, 2005. https://doi.org/10.3354/dao065257