Effects of temperature on pharmacokinetics of oxolinic acid in black rockfish, Sebastes schlegeli following oral administration

조피볼락, Sebastes schlegeli에 경구투여된 oxolinic acid의 약물동태에 미치는 수온의 영향

Effects of temperature ($13{\pm}1.5^{\circ}C$, $23{\pm}1.5^{\circ}C$) on the pharmacokinetic properties of oxolinic acid (OA) were studied after oral administration to cultured black rockfish, Sebastes schlegeli. Serum concentrations of OA were determined using HPLC-UV detector after a single dosage of 60 mg/kg body weight (average about 500 g). The peak serum concentrations of OA at $23{\pm}1.5^{\circ}C$ and $13{\pm}1.5^{\circ}C$ were $0.60{\mu}/ml$ at 30 h and $2.22{\mu}g/ml$ at 10 h post-dose, respectively. Better absorption of OA was noted at $13{\pm}1.5^{\circ}C$ compared to $23{\pm}1.5^{\circ}C$. The elimination of OA from serum was considerably faster at $23{\pm}1.5^{\circ}C$ than at $13{\pm}1.5^{\circ}C$. Both absorption and elimination of OA were affected significantly by temperature. The kinetic profile of absorption, distribution and elimination of OA in serum was analyzed by fitting to a two compartment model, with WinNonlin program. The AUC, Tmax and Cmax at $23{\pm}1.5^{\circ}C$ were $42.16{\mu}g{\cdot}h/m\ell$, 26.13 h and $0.43{\mu}g/ml$, respectively. The AUC, Tmax and Cmax at $13{\pm}1.5^{\circ}C$ were $131.98{\mu}g{\cdot}h/ml$, 8.81 h and $2.04{\mu}g/ml$, respectively.

Oxolinic acid를 사육수온($13{\pm}1.5^{\circ}C$, $23{\pm}1.5^{\circ}C$)에 따라서 조피볼락(평균체중 500 g)에 60 mg/kg의 농도로 1회 경구 투여한 다음, 경시적인 혈청내 잔류농도를 분석하였다. $23{\pm}1.5^{\circ}C$의 경우 투여 후 30시간째($0.60{\mu}g/ml$)에 최대혈중농도에 도달하였다. $13{\pm}1.5^{\circ}C$의 경우 투여 후 10시간째($2.22{\mu}g/ml$) 최대혈중농도에 도달하였다. OA의 조피볼락 혈중내 흡수양상과 소실정도는 사육수온에 크게 영향을 받았다. Two-compartment model로 해석하여 WinNonlin program을 이용, 약물동태학적 매개변수(parameter)를 조사하였다. $23{\pm}1.5^{\circ}C$의 경우, 혈장농도－시간곡선하 면적(AUC)은 $42.16{\mu}g{\cdot}h/ml$, 혈중최고농도의 도달시간(Tmax)은 26.13 h, 혈중최고농도(Cmax)는 $0.43{\mu}g/ml$, 예상약물소실시간(Et)은 230시간으로 계산되었다. $13{\pm}1.5^{\circ}C$의 경우, AUC는 $131.98{\mu}g{\cdot}h/ml$, Tmax는 8.81 h, Cmax는 $2.04{\mu}g/ml$, Et는 492시간으로 계산되었다.