Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Functional implications of gene expression analysis from rice tonoplast intrinsic proteins during seed germination and development

벼 종자에서 액포막 aquaporin (tonoplast intrinsic protein) 유전자의 발현과 기능

  • Huh, Sun-Mi (Bio-crops development Division, National Academy of Agricultural Science, RDA) ;
  • Lee, In-Sook (Bio-crops development Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Beom-Gi (Bio-crops development Division, National Academy of Agricultural Science, RDA) ;
  • Shin, Young-Seop (Rice research Division, National Institute of Crop Science, RDA) ;
  • Lee, Gang-Seop (Genomics Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Dool-Yi (Bio-crops development Division, National Academy of Agricultural Science, RDA) ;
  • Byun, Myung-Ok (Bio-crops development Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Dong-Hern (Bio-crops development Division, National Academy of Agricultural Science, RDA) ;
  • Yoon, In-Sun (Bio-crops development Division, National Academy of Agricultural Science, RDA)
  • 허선미 (농촌진흥청 국립농업과학원 신작물개발과) ;
  • 이인숙 (농촌진흥청 국립농업과학원 신작물개발과) ;
  • 김범기 (농촌진흥청 국립농업과학원 신작물개발과) ;
  • 신영섭 (농촌진흥청 국립식량과학원 답작과) ;
  • 이강섭 (농촌진흥청 국립농업과학원 유전자분석개발과) ;
  • 김둘이 (농촌진흥청 국립농업과학원 신작물개발과) ;
  • 변명옥 (농촌진흥청 국립농업과학원 신작물개발과) ;
  • 김동헌 (농촌진흥청 국립농업과학원 신작물개발과) ;
  • 윤인선 (농촌진흥청 국립농업과학원 신작물개발과)
  • Received : 2010.10.18
  • Accepted : 2010.11.02
  • Published : 2010.12.31
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Abstract

Rice seed maturation and germination involve drastic changes in water and nutrient transport, in which tonoplast aquaporins may play an important role. In the present study, gene expression profiles of 10 tonoplast intrinsic proteins (TIP) from rice were investigated by RT-PCR during seed development and germination. OsTIP3;1 and OsTIP3;2 were specifically expressed in mature seeds. Their transcript level rapidly decreased after onset of seed germination and gene expression was induced by ABA treatment. In contrast, expression of OsTIP2;1 and OsTIP4;3 was not seed specific as transcripts were found in vegetative tissues as well. Their respective transcript levels decreased at an early stage of seed development, whereas they increased at a later stage of seed germination and elongation of embryonic roots and shoots. When seed germination was inhibited by various stress conditions and ABA, expression of OsTIP2;1 and OsTIP4;3 was completely suppressed. In contrast, the expression level of OsTIP2;2 rapidly increased after seed imbibition and the transcript level was maintained under conditions inhibiting seed germination. These results implicate that tissue specific and developmental transcriptional regulation of OsTIPs in rice seeds depends on their specific function. In addition, OsTIPs can be discriminated by different potential phosphorylation and methylation sites in their protein structures. OsTIP3;1 and OsTIP3;2 possess unique phosphorylation signatures at their N-terminal domain, loop B and loop E, respectively. OsTIP2;1 and OsTIP4;3 have a potential methylation site at their Nterminal domain. This suggests that activity of specific tonoplast aquaporins may be regulated by post-translational modification as well as by transcriptional control.

종자 발달과 발아는 수분과 양분 함량의 급격한 변화를 수반하는 복합적인 과정이다. 본 연구에서는 유전자 발현과 단백질 구조 비교 분석을 통해 벼 종자의 발아와 발달과정에 관여하는 액포막 aquaporin (tonoplast intrinsic protein)을 규명하였다. OsTIP3;1, OsTIP3;2는 종자 특이적인 TIP로 종자가 성숙되는 시기에 발현되었다가, 종자가 발아하면서 전사체가 사라지는 양상을 보였으며, ABA 처리에 의해 발현이 유도되었다. 단백질 구조 예측 결과로부터 OsTIP3;1, OsTIP3;2가 단백질의 N-말단, B와 E loop에 다른 TIP와 뚜렷이 구분되는 인산화 잔기 특징을 확인하였다. OsTIP2;1과 OsTIP4;3은 종자가 발달하는 과정에서 유전자 발현이 감소하였다가, 종자 발아 후기에 뿌리와 배축의 신장이 활발한 시기에 발현이 급증하였다. 특히 OsTIP2;1은 뿌리에서 강한 발현을 보였으므로, 뿌리 생장에 필요한 팽압 공급에 중요한 기능을 할 것으로 제안된다. OsTIP2;1과 OsTIP4;3 단백질의 N-말단에는 특징적으로 메틸화 (methylation) 가능성이 높은 아미노산 잔기가 예측되었다. OsTIP2;2는 OsTIP2;1과는 달리 종자 침윤 후 7시간 이내에 발현이 빠르게 유도되며, 발아가 억제되는 조건에서도 유전자 발현이 유지되는 것으로 보아 종자의 초기 수화 과정에 관여할 것으로 추측된다. OsTIP2;2 단백질의 N-말단에는 OsTIP2;1에 존재하는 인산화 Ser 잔기와 메틸화 잔기가 결실된 특징을 보였다. 이런 결과들은 벼 종자의 발달과 발아 과정에서 나타나는 액포의 종류와 기능에 따라 서로 다른 TIP가 선택적으로 유전자 발현수준에서 조절되며, 인산화, 메틸화 등 단백질 수식에 의한 활성조절 기작 역시 매우 다르다는 것을 시사한다.

Keywords

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