Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Petasites japonicus and Momordica charantia L. Extracts on MC3T3-E1 Osteoblastic Cells

머위(Petasites japonicus)와 여주(Momordica charantia L.) 추출물의 MC3T3-E1 조골세포 증식 및 분화에 미치는 효과

  • Ji, Suk-Hee (Dept. of Food Science and Nutrition, Dong-A University) ;
  • Ahn, Do-Hwan (Dept. of Physiology, Kosin Medical College) ;
  • Jun, Mi-Ra (Dept. of Food Science and Nutrition, Dong-A University)
  • 지숙희 (동아대학교 식품영양학과) ;
  • 안도환 (고신대학교 의과대학 생리학교실) ;
  • 전미라 (동아대학교 식품영양학과)
  • Published : 2010.02.27
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Abstract

In this study, the effects of Petasites japonicus and Momordica charantia L. extracts on MC3T3-ET1 osteoblastic cells were investigated. Since the activity of osteoblastic cell is one of the important factors for bone formation, the cellular proliferation of osteoblast was evaluated by MTT and alkaline phosphatase (ALP) activity. Compared to control, the cell proliferation was elevated to 114% and 112% by the treatment of Petasites japonicus and Momordica charantia L. extracts, respectively at the concentration of $10\;{\mu}g/mL$. The cell differentiation was also measured by alkaline phosphatase (ALP) activity at 3, 7, 14, and 27 days treatments with one of the extracts, respectively. As results, the ALP activity was significantly increased at 3 days, compared to control (p<0.05). To evaluate the effect of Petasites japonicus and Momordica charantia L. extracts on bone nodule formation, MC3T3-E1 cells were cultured in $\alpha$-MEM for 3, 14, and 21 days and then stained by alizarin red. To determine the expression patterns of bone-related proteins during the MC3T3-E1 osteoblast-like cell differentiation, osteoblast cells were cultured in $\alpha$-MEM for 24 hr. RNA was extracted and RT-PCR analysis was performed to examine the expression of OPG, RANKL and osteocalcin. Petasites japonicus extract exhibited the significant increment of osteocalcin compared with the positive control, which suggests that Petasites japonicus may have beneficial effects on bone health through the proliferation of osteoblast cells.

본 연구를 통하여 여주 추출물은 세포 증식을 제외하고는 조골세포에 긍정적인 영향을 미치지 못하였지만 머위 추출물은 세포의 증식, ALP 활성, bone nodule의 형성이 대조군과 비슷한 결과를 나타내거나 높은 경향을 나타냄으로써 조골세포의 골 형성 과정인 증식, 기질의 성숙, 기질의 석회화의 세 단계에서 유효성을 증명하였다. 또한 OPG mRNA의 2배 이상의 증가는 조골세포의 골 형성에 주요 매개 물질로서 가능성이 있음을 밝혔다. 따라서 머위 추출물은 골수의 미세 환경에서 세포의 조절작용을 하는 물질로 여겨지며, 골다공증을 포함한 각종 골 결손 질환의 예방과 치료약 개발에 긍정적인 가능성을 제시할 것이라 사료된다.

Keywords

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