Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
권호 표지

Effects of Gwanjul8-bang on Collagen Induced Arthritis in DBA/1J Mice

관절8호방이 Collagen 유발 관절염에서 DBA/1J 생쥐에 미치는 영향

  • Kim, Jong-Kook (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dae-Jeon University) ;
  • Oh, Min-Seok (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dae-Jeon University)
  • 김종국 (대전대학교 한의과대학 한방재활의학과교실) ;
  • 오민석 (대전대학교 한의과대학 한방재활의학과교실)
  • Received : 2010.03.17
  • Accepted : 2010.04.01
  • Published : 2010.04.30
⟨ Previous Next ⟩

Abstract

Objectives : This study was carried out to know the effects of Gwanjul8-bang(here in after reffered to GJ8) on the inhibition of arthritis induced by collagen on DBA/1J mice. Methods : For this purpose, GJ8 was orally administered to mouse with arthritis induced by collagen II. Cytotoxicity, hepatotoxicity, arthritis index, value of immunocyte in draining lymph node(DLN) and paw joint, cytokine in serum were measured in vivo. Results : 1. The arthritis index was decreased significantly. 2. In total cell counts of DLN and paw joint, the cells in DLN increased significantly while there was a significant decrease in paw joint. 3. In lymph nodes, $CD19^+$, $CD3^+$, $CD4^+/CD25^+$ cells increased significantly, and $B220^+/CD23^+$ cells decreased significantly. 4. In joints, $CD3^+$, $CD4^+$, $CD11b^+/Gr-1^+$ cells decreased significantly. 5. The levels of $TNF-{\alpha}$, IL-6, IL-17, MCP-1 and vascular endothelial growth factor(VEGF) in serum was significantly decreased compared with control. 6. Anti-collagen II in serum was significantly decreased compared with control. 7. The degree of arthritis induced damage of joint of GJ8 group is slight compared with control group in histopathologic observation(hematoxylin & eosin(H&E), Masson-Trichrome(MT) staining). Conclusions : Comparison of the results for this study showed that GJ8 had immunomodulatory effects. So we expect that GJ8 should be used as a effective drugs for not only rheumatoid arthritis but also another auto-immune disease. Therefore there seems to be many clinical research needed in the future.

Keywords

References

  1. 해리슨 번역 편찬위원회.해리슨 내과학.서울: 정담출판사.2006:2148-57.
  2. 한방재활의학과학회. 한방재활의학과학. 서울:군자출판사. 2005:78-99.
  3. 대한정형외과학회. 정형외과학. 서울: 최신의학사. 2001:175-6.
  4. 이혜순. 류마티스 관절염의 유전적 요인. 대한내과학회지. 2008;75(5):515-24.
  5. 유호상. 관절염에 관한 韓醫學碩.博論文73編에 대한 分析.대전대학교대학원. 2001.
  6. 허준. 東醫寶鑑. 경상남도: 동의보감출판사. 2005 :1027.
  7. 최정식, 송태원. 鷄血藤이 collagen으로 유발된 생쥐의 관절염 억제에 관한 연구. 대한본초학회지. 2003;18(3):79-88.
  8. 서해경, 오민석, 김동희. 류마토이드 관절염 환자 활막세포에 대한 鷄血藤의 면역반응. 동의생리병리학회지. 2003;17(3):780-6.
  9. 조영주,김성훈,박종오,김동희. 豨薟이 RA병태 모델에서 관련인자 및 조직학적 변화에 미치는 영향. 동의생리병리학회지. 2003;17(2):403-11.
  10. 대한병리학회. 병리학. 서울:고문사. 1997:85, 1672,1166-70.
  11. 양형인. 류마토이드 관절염의 최신 치료. 경희의학. 2004;20(1):1-3.
  12. 서영일. 류마티스 관절염의 새로 개발된 치료제. 대한내과학회지. 2009;76(1):12-7
  13. 송정수. 류마티스 관절염 치료에서 종양괴사인자억제제 사용에 대한 고찰. 대한류마티스학회지. 2007;14(1):1-14.
  14. 송영욱. 최근 조명된 류마티스 관절염의 병태생리. 대한내과학회지. 2009;76(1):1-6.
  15. 최도영,이재동,백용현,이송실,유명철,한정수,양형인,박상도,유미현,박은경,박동석. 류마토이드 관절염에 대한 한약의 면역학적 연구동향. 대한침구학회지. 2004;21(4):177-96.
  16. Holmdahl R,Jansson L,Larsson A,Jonsson R.Arthritisin DBA/1 mice induced with passively transferred type II collagen immune serum, immunohistopathology and serum levelsofanti-typeIIcollagenauto-antibodies. Scand J Immunol.1990;31:147-57. https://doi.org/10.1111/j.1365-3083.1990.tb02754.x
  17. 곽중문,오민석,박종오,김동희. 疎風活血湯加味方이 collagen으로 유발된 생쥐의 관절염 억제에 관한 연구. 동의생리병리학회지. 2002; 16(4):810-7.
  18. 정수현,김순중. 三痺湯이 collagen으로 유발된 관절염에 미치는 영향. 한방재활의학과학회지. 2000;10(1):173-83.
  19. 정수현,김순중,서일복. 대강활탕이 collagen 으로 유발된 관절염에 미치는 영향. 한방재활의학과학회지. 2004;14(3):79-101
  20. 한주원,송영일,윤일지,오민석. 거습복합방이 류마토이트 관절염에 미치는 영향.한방재활의학과학회지. 2006;16(3):1-16
  21. 홍순성,윤일지,오민석.거풍제습탕의 류마토이드 관절염 치료효과에 대한 실험적 연구. 동의생리병리학회지. 2006;20(5):1241-4.
  22. 전국한의과대학 본초학교수 공편저. 본초학. 서울:영림사. 1994:151,219,272,448,536,540,578.
  23. 이규범. 임상병리 핸드북. 서울:고문사. 2003 :102-5,233-5.
  24. 임상류마티스학 편찬위원회. 임상류마티스학. 서울:한국의학사. 2006:195,218,229-32,235-6.
  25. 정태호,김정철,김문규. 최신면역학강의. 대구: 경북대학교출판부. 1996:9,10,22,25,77,193, 200,215,211-2,232-5,248,357.
  26. 김세종. 면역학. 서울:고려의학. 1994:3,8,18, 25-32,114,134,148-50,157-8,206.
  27. Varbanova BB. et al. Clinicalimmunology. New York:Mosby. 2001;4(136):18.
  28. 타다 토미오. 면역의 의미론. 서울:한울. 2007 :103.
  29. Jonathan K, Tal M, Ehud H,Iftach S, Ethan S, Michal S. Naturally occurring $CD4^{+}CD25^{+}$ regulatory T cellssuppressthe ability to withstand injury to the central nervous system. NAS. 2002;99(24):15620-5. https://doi.org/10.1073/pnas.232565399
  30. Charbonnier LM, van Duivenvoorde LM, Apparailly F,Cantos C,Han WG,Noel D, Duperray C,Huizinga TW,Toes RE, Jorgensen C, Louis-Plence P. Immature dendriticcell suppress collagen-induced arthritis by in vivo expansion of $CD49b^{+}$ regulatory T cell. JImmunol. 2006;177(6):3806-13. https://doi.org/10.4049/jimmunol.177.6.3806
  31. Gavin MA, Clarke SR, Negrou E, Gallegos A, Rudensky A. Homeostasis and anergy of $CD4^{+}/CD25^{+}$ suppressor T cells in vivo. NatImmunol. 2002;3(1):33-41.
  32. Rosenwasser LJ, Busse WW, Lizambri RG, Olejnik TA, Totoritis MC. Allergicasthma and an anti-CD23 mAb(IDEC-152):results of a phaseI, single-dose, dose-escalatingclinical trial. J Allergy Clin Immunnol. 2003;112(3) :563-70. https://doi.org/10.1016/S0091-6749(03)01861-X
  33. Mosby internatiional Ltd. Crash Couse. Immune blood and Lymphatic Systems. 서울:한우리. 2000:17-21.
  34. Johnson BA, Haines GK, Harlow LA, Koch AE. Adhension molecule expression in human synovial tissue. Arthritis Rheum.1993;36(2) :137-46. https://doi.org/10.1002/art.1780360203
  35. Hochberg, Silman, Smolen, Weinblatt, Weisman. Rheumatology. Philadelpia:Elsevier. 2008:848-51.
  36. Feldman M, Brennan FM, Maini RN. Rheumatoid Arthritis. Cell.1996;85:307-10. https://doi.org/10.1016/S0092-8674(00)81109-5
  37. Feldmann M, Maini RN. Anti-TNF alpha therapy of rheumatoid arthritis what have we learned, Annu Rev. Immunol. 2001;19 :163-96. https://doi.org/10.1146/annurev.immunol.19.1.163
  38. Houssiau FA, Devogelaer JP, Van Damme J, de Deux-chaisnes CN, Van Snick J. Interleukin-6 in synovial fluid and serum of patients with rheumatoid arthritis and other inflammatory arthritides. Arthritis Rheum.1988;32:784-8.
  39. 이준희, 김근태, 류선, 김주인, 백승훈, 김성일. 류마토이드 관절염 동물 모델에서 활막의 RANKL/OPG mRNA 발현비율 및 IL-17의 효과. 대한류마토이드학회지. 2006;13(2):123-31.
  40. Baggiolini M, Dewald B, Moser B. Human chemokines. Annu Rev. Immunol. 1997;15:675-705. https://doi.org/10.1146/annurev.immunol.15.1.675
  41. 고학재,유승아,우성룡,김해림,조철수,김완욱. 배양된 류마토이드 활액 단핵세포 및 활막 섬유아세포에서 Vascular Endothelial Growth Factor의 자극에 의한 Tumor Necrosis Factor-a 와 Interleukin-6 생성의 증가. 대한류마토이드학회지. 2004;11(2):133-43.
  42. Guo C, Piacentini L. Type I Collagen-induced MMP-2 Activation Coincides with Up-regulation of Membrane Type1-Matrix Metalloproteinase and TIMP-2 in Cardiac Fibroblasts. JBC. 2003;278(47):699-708. https://doi.org/10.1074/jbc.C200588200