Journal of International Academy of Physical Therapy Research (국제물리치료학회지)

International Academy of Physical Therapy Research (국제물리치료연구학회)
권호 표지

The Effects of NEES on PARP Expression and Cell Death in Rat Cerebral Cortex After Ischemic Injury

  • Received : 2010.06.11
  • Accepted : 2010.09.27
  • Published : 2010.10.31
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Abstract

The majority of strokes are caused by ischemia and result in brain tissue damage, leading to problems of the central nervous system including hemiparesis, dysfunction of language and consciousness, and dysfunction of perception. The purpose of this study was to investigate the effects of Poly(ADP-ribose) polymerase(PARP) on necrosis in neuronal cells that have undergone needle electrode electrical stimulation(NEES) prior to induction of ischemia. Ischemia was induced in male SD rats(body weight 300g) by occlusion of the common carotid artery for 5 min, after which the blood was reperfused. After induction of brain ischemia, NEES was applied to Zusanli(ST 36), at 12, 24 and 48 hours. Protein expression was investigated using immuno-reactive cells, which react to PARP antibodies in cerebral nerve cells, and Western blotting. The results were as follows: In the cerebral cortex, the number of PARP reactive cells after 24 hours significantly decreased(p<.05) in the NEES group compared to the GI group. PARP expression after 24 hours significantly decreased(p<.05) in the NEES group compared to the GI group. As a result, NEES showed the greatest effect on necrosis-related PARP immuno-reactive cells 24 hours after ischemia, indicating necrosis inhibition, blocking of neural cell death, and protection of neural cells. Based on the results of this study, NEES can be an effective method of treating dysfunction and improving function of neuronal cells in brain damage caused by ischemia.

Keywords

References

  1. Berger NA. Poly(ADP-ribose) in the cellular response to DNA damage. Radiat Res 1985; 101: 4-15. https://doi.org/10.2307/3576299
  2. Cho ZH, Chung SC, Jones JP, Park JB, Park H, Lee HJ, et al. New findings of the correlation between acupoints and corresponding brain cortices using functional MRI. Proc Natl Acad Sci USA 1998; 95: 2670-2673. https://doi.org/10.1073/pnas.95.5.2670
  3. Endres M, Scott G, Namura S, Salzman AL, Huang PL, Moskowitz MA, et al. Role of peroxynitrite and neuronal nitric oxide synthase in the activation of poly(ADP-ribose) synthetase in a murine model of cerebral ischemia-reperfusion. Neurosci Lett 1998; 248: 41-44. https://doi.org/10.1016/S0304-3940(98)00224-9
  4. Fisher M. The ischemic penumbra: identification evolution and treatment concepts. Cerebrovasc Dis 2004; 17(suppl 1): 1-6.
  5. Gao H, Guo J, Ahao P and Cheng J. The neuroprotective effect of electroacupuncture on focal cerebral ischemia in monkey. Acupunct Electro- Ther Res 2002; 27: 45-57. https://doi.org/10.3727/036012902816026112
  6. Ha HC, Snyder SH. Poly(ADP-ribose) polymerase is a mediator of necrotic cell death by ATP depletion. Proc Natl Acad Sci USA 1999; 96: 13978-13982. https://doi.org/10.1073/pnas.96.24.13978
  7. Hausmann R, Biermann T, Wiest I, Tubel J, Betz P. Neuronal apoptosis following human brain injury. Int J Legal Med 2004; 118(1): 32-36. https://doi.org/10.1007/s00414-003-0413-4
  8. Ikai K, Ueda K, Hayaishi O. Immunohistoche mical demonstration of poly(adenosine diphosphate- ribose) in nuclei of various rat tissues. J Histochem Cytochem 1998; 28: 670-676.
  9. Inoue I, Chen L, Zhou L, Zeng X and Wang H. Reoroduction of scalp acupuncture therapy on stroke in the model rats spontaneous hypertensive rat-stroke prone(SHR-SP). Neurosci Lett 2002; 333: 191-194. https://doi.org/10.1016/S0304-3940(02)01032-7
  10. Jang MH, Shin MC, Koo GS, Lee CY, Kim EH, Kim CJ. Acupuncture decreases nitric oxide synthase expression in periaqueductal gray area of rats with streptozotocin-induced diabetes. Neurosci Lett 2003; 337: 155-158. https://doi.org/10.1016/S0304-3940(02)01318-6
  11. Karczewski JM, Peters JG, Noordhoek J. Prevention of oxidant-induced cell death in Caco-2 colon carcinoma cells after inhibition of poly(ADP-ribose) polymerase and $Ca2^{+}$ chelation: involvement of a common mechanism. Biochem Pharmacol 1999; 57: 19-26. https://doi.org/10.1016/S0006-2952(98)00286-X
  12. Kato H, Konure K. Biochemical and molecular characteristics of the brain with developing cerebral infarction. Cellular and Molecular Neurobiol 1999; 19: 93-108. https://doi.org/10.1023/A:1006920725663
  13. Kim EH, Kim YJ, Lee HJ, Huh Y, Chung JH, Seo JC, et al. Acupuncture increase cell proliferation in dentate gyrus after transient global ischemia in gerbils. Neurosci Lett 2001; 277: 21-24.
  14. Kochanek PM, Hallenbeck JM, Polymorphonuc lear leukocytes and monocytes/macrophages in the pathogenesis of cerebral ischemia and stroke. Stroke 1992; 23: 1367-1379. https://doi.org/10.1161/01.STR.23.9.1367
  15. Lee YA. Patients' Lived Experience in Rehabilitating from Stroke. The Kor J Rehabili Nur 2001; 4: 1.
  16. Ng I, Yeo TT, Tang WY, Soong R, Ng PY, Smith DR. Apoptosis occurs after cerebral contusions in humans. Neurosurgery 2000; 46: 949-956.
  17. Nicoletti VG, Stella AM. Role of PARP under stress conditions; cell death or protection. Neurochem Res 2003; 28: 187-194. https://doi.org/10.1023/A:1022316914492
  18. Ogura T, Takenouchi N, Yamaguchi M, Matsukage A, Sugimura T, Esumi H. Striking similarity of the distribution patterns of the poly(ADP-ribose) polymerase and DNA polymerase beta among various mouse organs. Biochem Biophys Res Commun 1990; 172: 377-384. https://doi.org/10.1016/0006-291X(90)90683-E
  19. Runnerstam M, Bao F, Huang Y, Shi J, Gutierrez E, Hamberger A, Hansson H, Viano D, Haglid K. A model for diffuse brain injury by rotational acceleration, IL Effect on extracellular glutamate Intracranial pressure and neuronal apoptosis. J Neurotrauma 2001; 18(3): 259-273. https://doi.org/10.1089/08977150151070892
  20. Schraufstatter IU, Hyslop PA, Hinshaw DB, Spragg RG, Sklar LA, Cochrane CG. Hydrogen peroxide-induced injury of cells and its prevention by inhibitors of poly(ADP-ribose) polymerase. Proc Natl Acad Sci USA 1986; 83: 4908-4912. https://doi.org/10.1073/pnas.83.13.4908
  21. Sharp FR, Lu A, Tang Y, Millhorn DE. Multiple molecular penumbras after cerebral ischemia. J Cereb Blood Flow Metab 2000; 20: 1011-1032. https://doi.org/10.1097/00004647-200007000-00001
  22. Uchida S, Kagitani F, Suzuki A and Aikawa Y. Effect of acupuncture like stimulation on cortical cerebral blood flow in anesthetized rats. Jpn J Physiol 2000; 50: 495-507. https://doi.org/10.2170/jjphysiol.50.495
  23. Virag L, Salzman AL, Szabo C. Poly(ADP-ribose) synthetase activation mediates mitochondrial injury during oxidantinduced cell death, J Immunol 1998; 161: 3753-3759.
  24. Wallis RA, Panizzon KL, Henry D, Wasterlain CG. Neuroprotection against nitric oxide injury with inhibitors of ADP-ribosylation. Neuroreport 1993; 5: 245-248.
  25. Zhang J, Dawson VL, Dawson TM, Snyder SH. Nitric oxide activation of poly (ADP-ribose) synthetase in neurotoxicity. Science 1994; 263: 687-689. https://doi.org/10.1126/science.8080500
  26. Zhang J, Pieper A, Snyder SH. Poly(ADP-ribose) synthetase activation: an early indicator of neurotoxic DNA damage. J Neurochem 1995; 65: 1411-1414.