Evaluation of remineralization and acid resistance effect in fluoride varnish by Micro-computed tomography

미세전산화단층촬영술을 이용한 불소바니쉬의 재광화 및 내산성 평가

  • Oh, Han-Na (Dept. of Preventive and Public Health Dentistry) ;
  • Jeong, Seong-Soog (Dept. of Preventive and Public Health Dentistry) ;
  • Lee, Hye-Jin (Dept. of Preventive and Public Health Dentistry) ;
  • Youn, Hye-Jeong (Dept. of Dental Hygiene, Shinheung College University) ;
  • Jung, Eun-Ju (Dept. of Dental Hygiene, Cheongam University) ;
  • Ha, Myung-Ok (Dept. of Dental Hygiene, Gwangju Health College University)
  • 오한나 (전남대학교 치의학전문대학원 예방치과학교실) ;
  • 정성숙 (전남대학교 치의학전문대학원 예방치과학교실) ;
  • 이혜진 (전남대학교 치의학전문대학원 예방치과학교실) ;
  • 윤혜정 (신흥대학 치위생과) ;
  • 정은주 (청암대학 치위생과) ;
  • 하명옥 (광주보건대학 치위생과)
  • Received : 2010.10.01
  • Accepted : 2010.10.23
  • Published : 2010.10.30

Abstract

Objectives : The purpose of this investigation was to evaluate the remineralization and acid resistance in fluoride varnish by Micro-computed tomography (micro CT). Methods : Specimens of bovine teeth enamel were embedded in resin, polished and randomly divided into 3 groups (a control group, a NaF solution group, a fluoride varnish group). Each group has 3 specimens that was standardized according to Vickers hardness number (VHN). Specimens were immersed in demineralization solution for 72 hours. The control group had no treatment, the NaF solution group was treated by a 5% NaF solution for 4 minutes, and the fluoride varnish group was treated by a fluoride varnish for one hour. All specimens were subjected to a chemical pH cycling method for 14 days. After a chemical pH cycling method, the density were measured using micro CT. Then, specimens were immersed in each demineralization solution for 72 hours. After demineralization processed, the density were measured using micro CT. Results : 1. The density was significantly higher in the fluoride varnish and 5% NaF solution group than that of the control group after 14 days cycling (p<0.05). And the density value of the fluoride varnish group was higher than that of the 5% NaF solution, with no significant difference. 2. The differences of density after acid resistance treatment were statistically significant among 3 groups(p<0.05). Conclusions : It is suggested that fluoride varnish showed the remineralizing effect and acid resistance effect on the enamel, and micro CT could be used to evaluate the change of enamel lesion.

Keywords

References

  1. Lee YE, Baek HJ, Choi YH, et al. Comparison of remineralization effect of three topical fluoride regimens on enamel initial carious lesions. J Dent 2010;38(2):166-171. https://doi.org/10.1016/j.jdent.2009.10.002
  2. Adriaens ML, Dermaut LR, Verbeeck RM. The use of Fluor Protector, a fluoride varnish, as a caries prevention method under orthodontic molar bands. Eur J Orthod 1990;12(3):316-319.
  3. Ekstrand J, Koch G, Petersson LG. Plasma fluoride concentration and urinary fluoride excretion in children following application of the fluoridecontaining varnish duraphat. Caries Res 1980;14(4): 185-189. https://doi.org/10.1159/000260452
  4. 윤명옥, 이난영, 이상호. 불소바니쉬가 법랑질 탈회에 미치는 영향. 대한소아치과학회지 2008;35(3): 446-455.
  5. Peyron M, Matsson L, Birkhed D. Progression of approximal caries in primary molars and the effect of duraphat treatment. Scand J Dent Res 1992; 100(6):314-8.
  6. Twetman S, Petersson LG. Prediction of caries in pre-school children in relation to fluoride exposure. Eur J Oral Sci 1996;104(5-6):523-528. https://doi.org/10.1111/j.1600-0722.1996.tb00136.x
  7. Frostell G, Birkhed D, Edwardsson S, et al. Effect of partial substitution of invert sugar for sucrose in combination with duraphat treatment on caries development in preschool children: the Malmo Study. Caries Res 1991;25(4):304-310. https://doi.org/10.1159/000261381
  8. Grodzka K, Augustyniak L, Budny J, et al. Caries increment in primary teeth after application of duraphat fluoride varnish. Community Dent Oral Epidemiol 1982;10(2):55-59. https://doi.org/10.1111/j.1600-0528.1982.tb00362.x
  9. 조민정, 심형순, 이향님 외 4인. 불소바니쉬가 내산성 및 재광화에 미치는 영향. 한국치위생학회지 2009; 9(4):740-752.
  10. Hu W, Featherstone JD. Prevention of enamel demineralization: an in-vitro study using lightcured filled sealant. Am J Orthod Dentofacial Orthop. 2005;128(5):592-600. https://doi.org/10.1016/j.ajodo.2004.07.046
  11. 조난주, 이상호, 이난영. 불소바니쉬가 인공 우식 병소의 발생에 미치는 영향. 대한소아 치과학회지 2007; 34(4):613-622.
  12. Holmen L, Ogaard B, Rolla G, Thylstrup A. A polarized light and scanning electron microscope study of the effect of Duraphat treatment on in vivo caries. Scand J Dent Res 1986;94(6):521-9.
  13. 황지원. pH 순환 모형을 이용한 CPP-ACP 연고와 불소바니쉬의 재광화 효과 비교. 연세대학교 석사학위 논문 2008.
  14. Bergmans L, Van Cleynenbreugel J, Wevers M, Lambrechts P. A methodology for quantitative evaluation of root canal instrumentation using microcomputed tomography. Int Endod J 2001; 34(5):390-398. https://doi.org/10.1046/j.1365-2591.2001.00413.x
  15. 신영림, 김교한, 송근배. 불소용액양치 및 불소이온 도입법에 의한 불소도포가 초기법랑질 탈회병소의 재석회화에 미치는 영향. 대한구강보건학회지 1994; 18(2):441-457.
  16. 배익현, 김재문, 정태성, 김신. 글라스 아이오노머 수복재의 불소 유리 및 재흡수 양상. 대한소아치과학회지 2005;32(1):136-143.
  17. Arends J, Nelson DGA, Dijkman AG et al. Effects of various fluorides on enamel structure and chemistry: cariology today. Basel:Karger;1984:245-258.
  18. Ekstrand J, Koch G, Petersson LG. Plasma fluoride concentration and urinary fluoride excretion in children following application of the fluoride-containing varnish duraphat. Caries Res 1980;14(4):185-189. https://doi.org/10.1159/000260452
  19. Strohmenger L, Brambilla E. The use of fluoride varnishes in the prevention of dental caries: a short review. Oral Dis 2001;7(2):71-80. https://doi.org/10.1034/j.1601-0825.2001.70202.x
  20. Petersson LG, Twetman S, Dahlgren H, et al. Professional fluoride varnish treatment for caries control: a systematic review of clinical trials. Acta Odontol Scand 2004;62(3):170-176. https://doi.org/10.1080/00016350410006392
  21. Bawden JW. Fluoride varnish a useful new tool for public health dentistry. J Public Health Dent 1998; 58(4):266-269. https://doi.org/10.1111/j.1752-7325.1998.tb03007.x
  22. Todd MA, Staley RN, Kanellis MJ, Donly KJ, Wefel JS. Effect of a fluoride varnish on demineralization adjacent to orthodontic brackets. Am J Orthod Dentofacial Orthop 1999;116(2): 159-167. https://doi.org/10.1016/S0889-5406(99)70213-1
  23. Khattak MF, Conry JP, Ko CC. Comparison of three topical fluorides using computer imaging. J Clin Pediatr Dent 2005;30(2):139-144.
  24. Holm AK. Effect of a fluoride varnish(duraphat${\circledR}$) in preschool children. Community Dent Oral Epidemiol 1979;7(5):241-245. https://doi.org/10.1111/j.1600-0528.1979.tb01225.x
  25. Modeer T, Twetman S, Bergstrand F. Three-year study of the effect of fluoride varnish (duraphat) on proximal caries progression in teenagers. Scand J Dent Res 1984;92(5):400-407.
  26. 권지훈, 박호원, 이주현, 서현우. 공초점 레이저 주사 현미경을 이용한 불소바니쉬 재광화 효과의 분석. 대한소아치과학회지 2008;35(1):57-64.
  27. 김광현, 한세현, 김종철. Fluoride varnish와 acidulated phosphate fluoride gel이 인공우식 병소에 미치는 영향. 대한소아치과학회지 2001;28(1):159-165.
  28. 이혁상, 김영재, 김정욱, 장기택. 미세전산화단층촬영을 이용한 글라스 아이오노머 수복물의 인접면우식 재광화 효과에 관한 장기간 연구. J Korean Acad Pediatr Dent 2006;33(3):498-503.