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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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The production and immunostimulatory activity of double-stranded CpG-DNA

  • Park, Byoung-Kwon (Department of Microbiology, College of Medicine, Hallym University) ;
  • Kim, Dong-Bum (Department of Microbiology, College of Medicine, Hallym University) ;
  • Rhee, Jae-Won (Center for Medical Science Research, College of Medicine, Hallym University) ;
  • Kim, Min-Soo (Department of Microbiology, College of Medicine, Hallym University) ;
  • Seok, Hyun-Jeong (Department of Biochemistry, College of Natural Sciences, Chungbuk National University) ;
  • Choi, Soo-Young (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Park, Jin-Seu (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Lee, Young-Hee (Department of Biochemistry, College of Natural Sciences, Chungbuk National University) ;
  • Kwon, Hyung-Joo (Department of Microbiology, College of Medicine, Hallym University)
  • Published : 2010.03.31
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Abstract

CpG-DNA, which contains unmethylated CpG dinucleotides in the context of specific sequences, has remarkable and diverse immunological effects, including induction of proinflammatory cytokine expression and regulation of the Th1/Th2 immune response. Here, we examined the immunostimulatory activities of double-stranded (ds) CpG-DNA in the human B cell line RPMI8226. To investigate whether dsCpG-DNA stimulates immune cells, we constructed a plasmid containing repeated dsCpG-DNA and produced dsCpG-DNA by PCR amplification and EcoR I digestion. PCR-amplified dsCpG-DNA alone did not have immmunostimulatory activity. However, dsCpGDNA encapsulated with lipofectin induced IL-8 promoter activation, HLA-DRA expression, and IL-8 expression in a CG sequence-independent manner. The effects of encapsulated dsCpGDNA were independent of minor endotoxin contamination. These findings suggest the potential use of dsCpG-DNA as a therapy for immune response regulation.

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References

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