BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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High glucose induces differentiation and adipogenesis in porcine muscle satellite cells via mTOR

  • Yue, Tao (State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, China Agricultural University) ;
  • Yin, Jingdong (State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, China Agricultural University) ;
  • Li, Fengna (State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, China Agricultural University) ;
  • Li, Defa (State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, China Agricultural University) ;
  • Du, Min (Department of Animal Science, University of Wyoming)
  • 발행 : 2010.02.28
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The present study investigated whether the mammalian target of rapamycin (mTOR) signal pathway is involved in the regulation of high glucose-induced intramuscular adipogenesis in porcine muscle satellite cells. High glucose (25 mM) dramatically increased intracellular lipid accumulation in cells during the 10-day adipogenic differentiation period. The expressions of CCAAT/enhancer binding protein-$\alpha$ (C/EBP-$\alpha$) and fatty acid synthase (FAS) protein were gradually enhanced during the 10-day duration while mTOR phosphorylation and sterol-regulatory- element-binding protein (SREBP)-1c protein were induced on day 4. Moreover, inhibition of mTOR activity by rapamycin resulted in a reduction of SREBP-1c protein expression and adipogenesis in cells. Collectively, our findings suggest that the adipogenic differentiation of porcine muscle satellite cells and a succeeding extensive adipogenesis, which is triggered by high glucose, is initiated by the mTOR signal pathway through the activation of SREBP-1c protein. This process is previously uncharacterized and suggests a cellular mechanism may be involved in ectopic lipid deposition in skeletal muscle during type 2 diabetes.

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참고문헌

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