Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antiproliferation Effects of Germinated-Korean Rough Rice Extract on Human Cancer Cells

한국산 발아 벼 추출물의 여러 가지 암세포주에 대한 증식 억제 효과 비교

  • Kim, Hyun-Young (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Hwang, In-Guk (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Joung, Eun-Mi (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Kim, Tae-Myoung (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Kim, Dae-Joong (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Park, Dong-Sik (National Institute of Crop Science) ;
  • Lee, Jun-Soo (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Jeong, Heon-Sang (Dept. of Food Science and Technology, Chungbuk National University)
  • Published : 2010.03.31
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Abstract

This study was conducted to investigate the effects of 70% ethanol extracts of various germinated-rough rice cultivars: ('Ilpum', 'Goami2', 'Keunnun', 'Sulgaeng', 'Baegjinju', and 'Heugkwang') on proliferation of human cancer cell lines (MKN-45, HCT116 and NCI-H460). Antiproliferation effects of rough rice on different cancer cell lines were higher in after germination than before germination. The viability of HCT-116 colon cancer cells was lowest at 18.89% in after germination of 'Heugkwang' at 1.0 mg/mL. The cell viability of MKN-45 lung cancer cells and MKN-45 stomach cancer cells were in the range of 5~10% in after germination of 'Ilpum', 'Goami2', 'Baegjinju', and 'Sulgaeng', and 'Heugkwang' at 1.0 mg/mL. These results suggest that germinated rough rice might have a potential preventive effect on human cancer cells.

한국산 벼 6종(일품벼, 백진주벼, 설갱벼, 고아미2호, 거대배아벼 및 흑광벼)의 발아 및 무발아 에탄올 추출물에 대한 암세포주 증식 억제효과를 위암세포주(MKN45), 대장암세포(HCT116) 및 폐암세포(NCI-H460)에 대하여 살펴 본 결과 일부 품종의 경우 발아 후에 1 mg/mL 농도에서 암세포증식 억제효과가 증가하였다. 대장암세포주에 대한 성장억제 효과가 가장 좋았던 품종은 흑광벼로 발아 에탄올 추출물 18.89%의 생존율을 보였으며, 폐암세포주에 대해서는 일품, 고아미2호, 백진주 및 설갱벼 발아 에탄올 추출물은 5~10%의 생존율을 보였다. 위암세포에 대해서는 일품, 고아미2호, 백진주 및 설갱벼에 대하여 5~10%의 생존율을 보여 대조군에 비해 암세포주 증식 억제효과가 있는 것으로 나타났다. 본 연구결과 벼를 발아시킬 경우 항암활성이 증가함을 알 수 있었으며, 발아 후 벼의 성분분석 및 추후 항암실험의 다양한 지표를 활용하여 항암활성을 입증하는 연구가 수행되어야 할 것으로 판단된다.

Keywords

References

  1. Kwak TS, Teo JH. 2004. Varietal variation of ripening and physico-chemical properties in different rice ecotypes. J Korean Intl Agric 16: 130-135.
  2. Ministry of Agriculture and Forestry Republic of Korea. 2005. Agricultural and Forestry Statistical Yearbook, Agricultural and Forestry Major Statistics.
  3. Kim LS, Son YK, Son JR, Hur HS. 2001. Effect of germination condition and drying methods on physicochemical properties of sprouted brown rice. J Korean Crop Sci 46: 221-228.
  4. Ministry of Agriculture and Forestry Republic of Korea. 2005. Agricultural and Forestry Statistical Yearbook, Agricultural and Forestry Major Statistics, Stock Farm Product Annual per Consumption Trend.
  5. Lee YR, Kim JY, Woo KS, Hwang IG, Kim KH, Kim KJ, Kim JH, Jeong HS. 2007. Changes in the chemical and functional components of Korean rough rice before and after germination. Food Sci Biotechnol 16: 1006-1010.
  6. Lee MH, Son HS, Choi OK, Oh SK, Kwon TB. 1994. Changes in physico-chemical properties and mineral contents during buck-wheat germination. J Korean Food Nutr 7: 267-273.
  7. Cho BM, Yoon SK, Kim WJ. 1985. Changes in amino acid and fatty acids composition during germination of rapeseed. J Korean Food Sci 45: 87-91.
  8. Choi KS, Kim ZU. 1985. Changes in lipid components during germination of mungbean. J Korean Food Sci Technol 17: 271-275.
  9. Colmenarse DRAS, Bressani R. 1990. Effect of germination on the chemical composition and nutritive values of amaranth grain. Cereal Chem 67: 519-523.
  10. Kim IS, Kwon TB, Oh SK. 1985. Study on the chemical change of general composition fatty acids and mineral contents during germination. J Korean Food Sci Technol 17: 371-376.
  11. Hsu D, Leung HK, Finney PL, Morad MM. 1980. Effect of germination on nutritive value and baking properties of dry peas, lentils and faba beans. J Food Sci 45: 87-91. https://doi.org/10.1111/j.1365-2621.1980.tb03877.x
  12. Ikeda K, Arioka K, Fujii S, Kusano T, Oku M. 1984. Effect on buck-wheat protein quality of seed germination and changes in trypsin inhibitor content. Cereal Chem 61: 236-240.
  13. Ishiyama M, Tominaga H, Shiga M, Sasamoto K, Ohkura Y, Ueno K. 1996. A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet. Biol Pharm Bull 19: 1518-1520. https://doi.org/10.1248/bpb.19.1518
  14. Hong MY, Seeram NP, Zhang Y, Heber D. 2008. Anticancer effects of Chinese red yeast rice versus monacolin K alone on colon cancer cells. J Nutr Biochem 19: 448-458. https://doi.org/10.1016/j.jnutbio.2007.05.012
  15. Manson MM, Gescher A, Hudson EA, Plummer SM, Squires MS, Prigent SA. 2000. Blocking and suppressing mechanism of chemoprevention by dietary constituents. Toxicology Lett 112: 499-505. https://doi.org/10.1016/S0378-4274(99)00211-8
  16. Lee DJ, Kim KH, Kang JH, Lee TS, Kim HW. 2006. Antioxidant and anticancer activities of methanolic extracts in grains of the Korean rice laneraces. J Korean Intl Agric 18: 264-269.
  17. Kuno T, Hirose Y, Yamada Y, Hata K, Quiang SH, Asano N, Oyama T, Zhi H, Iwasake T, Kobayashi H, Mori H. 2006. Chemoprevention of mouse urinary bladder carcinogenesis by fermented brown rice and rice bran. Oncol Rep 15: 533-538.
  18. Tian S, Nakamura K, Cui T, Kayahara H. 2005. High-performance liquid chromatographic determination of phenolic compounds in rice. J Chromatogr A 1063: 121-128. https://doi.org/10.1016/j.chroma.2004.11.075
  19. Kang BR, Park MJ, Lee HS. 2006. Germination dependency of antioxidative activities in brown rice. J Korean Soc Food Sci Nutr 35: 389-394. https://doi.org/10.3746/jkfn.2006.35.4.389
  20. Craig WJ. 1997. Phytochemicals: guardians of our health. J Am Diet Assoc 97: S119-S204. https://doi.org/10.1016/S0002-8223(97)00721-9
  21. Adom KK, Liu RH. 2002. Antioxidant activity of grains. J Agric Food Chem 50: 6182-6187. https://doi.org/10.1021/jf0205099
  22. Kang MY, Kim SI, Koh HJ, Chin JH, Nam SH. 2004. Antioxidative activity of germinated specialty rices. J Korean Food Sci Technol 36: 624-630.

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