Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Artemisia princeps Extract on Bone Metabolism

애엽 추출물이 골 대사에 미치는 영향

  • 이승민 ((주)KMSI 부설 한국의과학연구소) ;
  • 김명규 ((주)KMSI 부설 한국의과학연구소) ;
  • 이승연 ((주)KMSI 부설 한국의과학연구소) ;
  • 강태훈 ((주)KMSI 부설 한국의과학연구소)
  • Published : 2010.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Artemisia princeps has been utilized as a traditional medicine for a variety of diseases in Korea. In this study, we investigated the effects of Artemisia princeps extract (APE) on bone metabolism both in vitro using primary mouse bone marrow-derived macrophage and in vivo using ovariectomized rats. APE decreased the number of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells and TRAP activity. Also, APE inhibited bone resorptive activity of differentiated osteoclasts. In ovariectomized rats, APE alleviated the decrease in the trabecular bone mineral density. These results showed that APE might be useful for the prevention of postmenopausal bone loss.

본 연구에서는 마우스 골수유래 파골세포와 난소절제 동물모델을 이용하여 애엽 추출물이 골대사에 미치는 영향을 살펴보았다. 애엽 추출물이 파골세포의 분화 및 활성에 미치는 영향을 알아보기 위하여 마우스 골수유래 전구세포에 M-CSF와 RANKL을 처리하여 파골세포로의 분화를 유도한 후, TRAP 염색법을 실시하고 TRAP 활성도를 측정하였다. 그 결과 애엽 추출물은 TRAP 양성 다핵세포의 형성과 TRAP 활성을 농도 의존적으로 감소시켰으며, 파골세포의 골흡수 또한 애엽 추출물의 최고농도($30\;{\mu}g/mL$) 처리 시약 90%까지 억제되는 것을 확인하였다. 난소절제 동물모델을 이용한 실험에서도 애엽 추출물이 난소절제에 따른 급격한 골밀도 감소를 막아준다는 사실을 확인할 수 있었다. 이상의 결과로 미루어 볼 때, 애엽 추출물이 에스트로겐 분비 감소로 인하여 급격한 골 손실이 나타나는 폐경기 여성의 골다공증 예방에 효과가 있을 것으로 사료된다.

Keywords

References

  1. Vaananen HK, Zhao H, Mulari M, Halleen JM. 2000. The cell biology of osteoclast function. J Cell Sci 113: 377-381.
  2. Suda T, Nakamura I, Jimi B, Takahashi N. 1997. Regulation of osteoclast function. J Bone Mineral Res 12: 869-879. https://doi.org/10.1359/jbmr.1997.12.6.869
  3. Canalis E, McCarthy T, Centrella M. 1988. Growth factors and the regulation of bone remodeling. J Clin Invest 81: 277-281. https://doi.org/10.1172/JCI113318
  4. Jilka RL. 1998. Cytokines, bone remodeling and estrogen deficiency. Bone 23: 75-81. https://doi.org/10.1016/S8756-3282(98)00077-5
  5. Boyle WJ, Simonet WS, Lacey DL. 2003. Osteoclast differentiation and activation. Nature 423: 337-342. https://doi.org/10.1038/nature01658
  6. Teitelbaum SL. 2000. Bone resorption by osteoclasts. Science 289: 1504-1508. https://doi.org/10.1126/science.289.5484.1504
  7. Takayanagi H. 2005. Mechanistic insight into osteoclast differentiation in osteoimmunology. J Mol Med 83: 170-179. https://doi.org/10.1007/s00109-004-0612-6
  8. Lacey DL, Timms R, Tan HL, Kelly MJ, Dunstan CR, Burgess T, Elliot R, Colonboro A, Elliot G, Scully S. 1998. Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation. Cell 93: 165-176. https://doi.org/10.1016/S0092-8674(00)81569-X
  9. Takayanagi H, Kim S, Koga T, Nishina H, Isshiki M, Yoshida H, Saiura A, Isobe M, Yokochi T, Inoue J, Wagner EF, Mak TW, Kodama T, Taniguchi T. 2002. Induction and activation of the transcription factor NFATc1 (NFAT2) integrate RANKL signaling in terminal differentiation of osteoclasts. Dev Cell 3: 889-901. https://doi.org/10.1016/S1534-5807(02)00369-6
  10. Lee J, Lee SH, Choi Y, Kim N. 2007. The negative role of IdS in osteoclastogenesis. Adv Exp Med Biol 602: 23-31. https://doi.org/10.1007/978-0-387-72009-8_3
  11. Takayanagi H. 2007. Osteoimmunology: shared mechanisms and crosstalk between the immune and bone systems. Nat Rev Immunol 7: 292-304. https://doi.org/10.1038/nri2062
  12. Bae KH. 2007. The medicinal plants of Korea. Kyo-hak publishing Co, Seoul, Korea. p 490.
  13. Min SW, Kim NJ, Baek NI, Kim DH. 2009. Inhibitory effects of eupatilin and jaceosidin isolated from Artemisia princeps on carrageenan-induced inflammation in mice. J Ethnopharmacol 125: 487-500. https://doi.org/10.1016/j.jep.2009.06.026
  14. Kim MJ, Han JM, Jin YY, Baek MH, Chung HG, Choi MS, Lee KT, Sok DE, Jeong TS. 2008. In vitro antioxidant and anti-inflammatory activities of Jaceosidin from Artemisia princeps Pampanini cv. Sajabal. Arch Pharm Res 31: 429-437. https://doi.org/10.1007/s12272-001-1175-8
  15. Ryu JH, Lee HJ, Jeong YS, Ryu SY, Han YN. 1998. Yomogin, an inhibitor of nitric oxide production in LPS-activated macrophages. Arch Pharm Res 21: 481-484. https://doi.org/10.1007/BF02974648
  16. Kang YJ, Jung UJ, Lee MK, Kim HJ, Jeon SM, Park YB, Chung HG, Baek NI, Lee KT, Jeong TS, Choi MS. 2008. Eupatilin, isolated from Artemisia princeps Pampanini, enhances hepatic glucose metabolism and pancreatic beta-cell function in type 2 diabetic mice. Diabetes Res Clin Pract 82: 25-32. https://doi.org/10.1016/j.diabres.2008.06.012
  17. Sarath VJ, So CS, Won YD, Gollapudi S. 2007. Artemisia princeps var orientalis induces apoptosis in human breast cancer MCF-7 cells. Anticancer Res 27: 3891-3898.
  18. Park EY, Lee KW, Lee HW, Cho YW, Baek NI, Chung HG, Jeong TS, Choi MS, Lee KT. 2008. The ethanol extract from Artemisia princeps Pampanini induces p53-mediated G1 phase arrest in A172 human neuroblastoma cells. J Med Food 11: 237-245. https://doi.org/10.1089/jmf.2007.609
  19. Han JM, Kim MJ, Baek SH, An S, Jin YY, Chung HG, Baek NI, Choi MS, Lee KT, Jeong TS. 2009. Antiatherosclerotic effects of Artemisia princeps Pampanini cv. Sajabal in LDL receptor deficient mice. J Agric Food Chem 57: 1267-1274. https://doi.org/10.1021/jf802639y
  20. Muhlbauer RC, Lozano A, Reinli A. 2002. Onion and a mixture of vegetables, salads, and herbs affect bone resorption in the rat by a mechanism independent of their base excess. J Bone Mineral Res 17: 1230-1236. https://doi.org/10.1359/jbmr.2002.17.7.1230
  21. Palacios VG, Robinson L, Borysenko CW, Lehmann T, Kalla SE, Blair HC. 2005. Negative regulation of RANKLinduced osteoclastic differentiation in raw264.7 cells by estrogen and phytoestrogens. J Biol Chem 280: 13720-13727. https://doi.org/10.1074/jbc.M410995200
  22. Kim SW, Kim HG, Lee BE, Hwand HH, Baek DH, Ko SY. 2006. Effects of mushroom, Pleurotus eryngii , extracts on bone metabolism. Clin Nutr 25: 166-170. https://doi.org/10.1016/j.clnu.2005.10.014
  23. Seo BI, Ku SK, Cha EM, Park JH, Kim JD, Choi HY, Lee HS. 2005. Effect of Mornidae radix extracts on experimental osteoporosis in sciatic neuroectomized mice. Phytother Res 19: 231-238. https://doi.org/10.1002/ptr.1683
  24. Kim OJ, Kang KK, Kim DH, Baik NG, Ahn BO, Kim WB, Yang JI. 1996. Four-week oral toxicity study of DA-9601, an antiulcer agent of Artemisia spp. extract in rats. J Appl Pharmacol 4: 354-363.
  25. Liu ZG, Zhang R, Li C, Ma X, Liu L, Wang JP, Mei QB. 2009. The osteoprotective effect of Radix dipsaci extract in ovariectomized rats. J Ethnopharmacol 123: 74-81. https://doi.org/10.1016/j.jep.2009.02.025
  26. Zhang R, Liu ZG, Li C, Hu SJ, Liu L, Wang JP, Mei QB. 2009. Du-Zhong (Eucommia ulmoides Oliv.) cortex extract prevent OVX-induced osteoporosis in rats. Bone 45: 553-559. https://doi.org/10.1016/j.bone.2008.08.127
  27. Xie F, Wu CF, Zhang Y, Yao XS, Cheung PY, Chan AS, Wong MS. 2005. Increase in bone strength by Sambucus williamsii HANCE in ovariectomized rats. Biol Pharm Bull 28: 1879-1885. https://doi.org/10.1248/bpb.28.1879
  28. Zhang Y, Li XL, Lai WP, Chen B, Chow HK, Wu CF, Wang NL, Yao XS, Wong MS. 2007. Anti-osteoporotic effect of Erythrina variegata L. in ovariectomized rats. J Ethnopharmacol 109: 165-169. https://doi.org/10.1016/j.jep.2006.07.005
  29. Shirke SS, Jadhav SR, Jagtap AG. 2009. Osteoprotective effect of Phaseolus vulgaris L. in ovariectomy-induced osteopenia in rats. Menopause 16: 589-596. https://doi.org/10.1097/gme.0b013e31818e64c4
  30. Shirke SS, Jadhav SR, Jagtap AG. 2008. Methanolic extract of Cuminum cyminum inhibits ovariectomy-induced bone loss in rats. Exp Biol Med 233: 1403-1410. https://doi.org/10.3181/0803-RM-93
  31. Syed Z, Khan A. 2002. Bone densitometry: applications and limitations. J Obstet Gynaecol Can 24: 476-484. https://doi.org/10.1016/S1701-2163(16)31095-7
  32. Boutroy S, Bouxsein ML, Munoz F, Dalmas PD. 2008. In vivo assessment of trabecular bone microarchitecture by high-resolution peripheral quantitative computed tomography. J Clin Endocrinol Metab 90: 6508-6515. https://doi.org/10.1210/jc.2005-1258
  33. Zhang Y, Lai WP, Leung PC, Wu CF, Wong MS. 2007. Short to mid-term effects of ovariectomy on bone turnover, bone mass and bone strength in rats. Biol Pharm Bull 30: 898-903. https://doi.org/10.1248/bpb.30.898

Cited by

  1. Cytotoxicity and Antioxidative Activity of Artemisiae Argi Folium Alcoholic Extracts and Their Fractions vol.19, pp.6, 2011, https://doi.org/10.7783/KJMCS.2011.19.6.421
  2. 국내 한방 패치 특허 현황에 대한 분석연구 vol.22, pp.1, 2018, https://doi.org/10.25153/spkom.2018.22.1.004
  3. 갈색거저리 유충 오일이 MG-63 조골세포 분화에 미치는 영향 vol.29, pp.9, 2010, https://doi.org/10.5352/jls.2019.29.9.1027
  4. RANKL 유도된 파골세포 분화에 대한 풀무치 에탄올 추출물의 분화 억제 효과 vol.29, pp.10, 2019, https://doi.org/10.5352/jls.2019.29.10.1104