DOI QR코드

DOI QR Code

Skin UVB Photo Aging Effect from Extract of Fermented Reynoutria elliptica

호장근(Reynoutria elliptica) 발효 추출물의 UVB에 의한 피부 광노화 억제효과

  • 임애경 ((재)대구테크노파크 바이오산업지원센터) ;
  • 정유정 (인제대학교 식품생명과학부) ;
  • 김길수 (경북대학교 수의과대학) ;
  • 김용해 (신화제약연구소(주)) ;
  • 곽정훈 (신화제약연구소(주)) ;
  • 홍주헌 (대구가톨릭대학교 외식식품산업학부) ;
  • 김학윤 (계명대학교 환경대학) ;
  • 김대익 ((재)대구테크노파크 바이오산업지원센터)
  • Published : 2010.03.31

Abstract

To evaluate the skin aging inhibition effect of fermented Reynoutria elliptica, skin aging model was produced by the irradiation of UVB to hairless mice for 5 weeks. The skin erythema index for the positive control (PC), not fermented Reynoutria elliptica extract (NFR), and fermented Reynoutria elliptica extract (FR-500, 1000, 2000) groups were lower than that of the control group. However, both lipid and water capacity for the PC and FR groups were higher than those of the control group. Collagen fibers in dermis of the FR groups were almost intact with a regular arrangement which were similar to the normal (NO) group. Also, relatively much less number of mast cells and inflammatory cells were found in FR groups. The skin TBARS contents and XO activity in the FR group were significantly lower than the control group. The activities of GSH, SOD and CAT for the FR groups were significant higher than the control group. Therefore, fermented Reynoutria elliptica extract can be practically useful for the prevention or improvement of skin aging in terms of health promotion and beauty for the people.

호장근 발효 추출물을 대상으로 피부노화 억제활성을 살펴보고자 발효 전후의 호장근 추출물을 대상으로 피부 홍반도, 유수분 함량, 형태학 조직병리학적 검사, GSH, TBARS 그리고 XO, SOD, CAT 등의 항산화 효소활성을 측정하였다. 피부 홍반도에 있어서는 대조군에 비해 호장근 발효 추출물 처리군에서 뛰어난 홍반 감소 효과를 확인할 수 있었으며, 유수분 함량에 있어서도 FR-1000군이 유분함량은 $21.75{\pm}0.72$, 수분함량은 $55.13{\pm}1.07$로 CO군에 비해 유의하게 높은 함량을 보였다. 또한 형태학적으로는 FR을 처리한 실험군에서 피부의 주름이 현저하게 감소되었으며, 조직병리학적 검사에서도 표피 및 진피의 화농과 염증의 치료에 상당한 효과가 있음을 확인하였다. 또한 호장근 발효 추출물의 농도가 증가할수록 GSH는 증가하고 TBARS와 XO의 활성은 감소하였으며, 항산화 효소인 SOD, CAT 활성은 농도 의존적으로 증가함을 확인할 수 있었다. 이러한 결과들을 종합하여 볼 때 UVB 조사로 노화가 유발된 hairless mouse에서 호장근 발효 추출물의 도포가 노화된 피부에 염증반응을 약화시키며 유수분 함량을 증가시켜 피부장벽의 손상을 방지하며, 항산화 효소 및 resveratrol과 같은 항산화 물질을 증가시켜 항노화 기능성을 가지는 것으로 판단된다. 이로써 호장근 발효 추출물은 피부노화 예방 및 기능성 화장품 원료로서의 사용 가능성을 제시하는 바이다.

Keywords

References

  1. Kim EJ, Ahn SY, Nam GW, Lee HK, Moon SJ, Kim YM, Oh MS, Kim NS. 2006. The anti-aging effect of the cosmetic products containing the needles of red pine on human skin. Kor J Herbology 21: 25-31.
  2. Kim YC, Min KJ, Sin JS, Gang SR, Kim PS. 2008. The effects of geranium oil on the inhibition of skin aging in hairless mice (I). J Kor Soc Cosm 14: 495-503.
  3. Darr D, Fridovich I. 1994. Free radical in cutaneous biology. J Invest Dermatol 102: 671-675. https://doi.org/10.1111/1523-1747.ep12374036
  4. Hanawalt PC, Cooper PK, Ganesan AK, Smith CA. 1979. DNA repair in bacteria and mammalian cells. Ann Rev Biochem 48: 783-836. https://doi.org/10.1146/annurev.bi.48.070179.004031
  5. Lee TK, Kim JH, So JN. 2003. Emodin from Polygonum cuspidatum showed angiogenesis inhibiting activity in vitro. J Korean Soc Agric Chem Biotechnol 46: 50-54.
  6. Lee IS, Im HG, Lee SG. 2003. Growth inhibition of Helicobacter pylorio by Reynoutria elliptica Migo. Korean J Food Sci Technol 35: 1182-1187.
  7. Chi HJ, Choi JR, Yu SC. 1982. Pharmacognostical studies on "Ho-Jang" (III). Phytochemical study of the rhizome of Polygonum ellipicum Migo. Korean J Phytochem 13: 145-152.
  8. Park YS, Rho YS, Kim SK. 1984. Studies on antibacterial activity of several herb medicinal Tars-Reynoutriae radix, Sanguisorbae radix and Albizziae cortex. Bull Pharm Sci 12: 61-66.
  9. Ellman GL. 1959. Tissue sulfhydryl group. Arch Biochem Biophys 82: 70-77. https://doi.org/10.1016/0003-9861(59)90090-6
  10. Ohkawa H, Ohishi N, Yake K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95: 351-358. https://doi.org/10.1016/0003-2697(79)90738-3
  11. Stripe F, Della CE. 1969. The regulation of rat liver xanthine oxidase. Conversion in vitro of the enzyme activity from dehydrogenase (typeD) oxidase (type O). J Biol Chem 244: 3855-3863.
  12. Martin JP, Dailey M, Sugarman E. 1987. Negative and positive assays of superoxide dismutase based on hematoxylin autoxidation. Arch Biochem Biophys 255: 329-336. https://doi.org/10.1016/0003-9861(87)90400-0
  13. Abei H. 1974. Catalase. In Methods of Enzymatic Analysis. Bergmeyer, HU ed. Academic press, New York, USA. Vol 2, p 673.
  14. Soter NA. 1990. Acute effects of ultraviolet irradiation the skin. Dermatol 9: 11-15.
  15. Chiba K, Kawakami K, Sone T, Onoue M. 2003. Characteristics of skin wrinkling and dermal changes induced by repeated application of squalene monohydroperoxide to hairless mouse skin. Skin Pharmacol Appl Skin Physiol 16: 242-251. https://doi.org/10.1159/000070847
  16. Sheu SJ, Wu TT. 2009. Resveratrol protects against ultraviolet A-mediated inhibition of the phagocytic function of human retinal pigment epithelial cells via large-conductance calcium-activated potassium channels. Kaohsiung J Med Sci 25: 381-388. https://doi.org/10.1016/S1607-551X(09)70531-5
  17. Ray PS, Maulik G, Cordis GA, Bertelli AAE, Bertelli A, Das DK. 1999. The red wine antioxidant resveratrol protects isolated rat hearts from ischemia reperfusion injury. Free Radic Biol Med 27: 160-169. https://doi.org/10.1016/S0891-5849(99)00063-5
  18. Kligman LH, Akin FH, Kligman AM. 1982. Prevention of ultraviolet damage to the dermis of hairless mice by sunscreens. J Invest Dermatol 78: 181-189. https://doi.org/10.1111/1523-1747.ep12506359
  19. Mononey SJ, Edmonds SH, Giddens LD, Leaen DB. 1992. The hairless mouse model of photoaging: evaluation of the relationship between dermal elastin, collagen, skin thickness and wrinkles. Photochem Photobiol 56: 505-511. https://doi.org/10.1111/j.1751-1097.1992.tb02194.x
  20. Kligman LH, Crosby MJ, Higley H. 1993. The UVB-irradiated hairless mouse as a model for the evaluation of injecable collagen implant. J Dermatol Treat 1: 27-31.
  21. Yang ES, Hong RH, Kang SM. 2007. The effects of genistein on the proliferation and type I pN collagen synthesis in aged normal human fibroblasts. Kor J Microbiol Biotechnol 35: 316-324.
  22. Gedik E, Girgin S, Ozturk H, Obay BD, Ozturk H, Buyukbayram H. 2008. Resveratrol attenuates oxidative stress and histological alterations induced by liver ischemia/reperfusion in rats. World J Gastroenterol 14: 7101-7106. https://doi.org/10.3748/wjg.14.7101
  23. Im M. 1985. Effects of superoxide dismutase and allopurinol in survival of acute island skin flaps. Ann Surgery 201: 357-359. https://doi.org/10.1097/00000658-198503000-00018
  24. Manson PN. 1983. The role of oxygen free radicals in ischemic tissue injury in island skin flaps. Ann Surgery 198: 87-90. https://doi.org/10.1097/00000658-198307000-00017
  25. Morel DW, Chisolm GM. 1989. Antioxidative treatment of diabetic rats inhibits lipoprotein oxidation and cytotoxicity. J Lipid Res 30: 1827-1834.
  26. Deisseroth A, Dounce AL. 1970. Catalase physical and chemical properties, mechanism of catalysis and physiological role. Physiol Rev 50: 3-24.

Cited by

  1. Antioxidant and antityrosinase activity of fermented silkworm hemolymph vol.52, pp.2, 2014, https://doi.org/10.7852/jses.2014.52.2.159
  2. Skin Regeneration Effect of the Glycosaminoglycans from Liparis tessellatus eggs 2017, https://doi.org/10.1016/j.ijbiomac.2017.08.182
  3. Improving the efficacy of Lespedeza cuneata ethanol extract on ultraviolet-induced photoaging vol.21, pp.2, 2014, https://doi.org/10.11002/kjfp.2014.21.2.264
  4. Inhibitory Effects of Lespedeza cuneata Ethanol Extract on Ultraviolet-Induced Photo Aging vol.41, pp.11, 2012, https://doi.org/10.3746/jkfn.2012.41.11.1540
  5. Antioxidant and Whitening Activities of Various Cultivars of Korean Unripe Peaches (Prunus persica L. Batsch) vol.41, pp.2, 2012, https://doi.org/10.3746/jkfn.2012.41.2.156
  6. 노간주나무(Juniperus rigida Sieb.)의 주름개선 효과 vol.41, pp.4, 2010, https://doi.org/10.4014/kjmb.1307.07004
  7. Antimicrobial and Anti-Inflammatory Effects of the Ethanol Extract of Polygonum cuspidatum as a Mouthwash Component vol.16, pp.4, 2010, https://doi.org/10.1177/1934578x211005255