Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Optimization of Extraction Conditions for Extracts from Cucurbita moschata Duch. by Response Surface Methodology

반응표면분석에 의한 늙은 호박 추출물의 추출조건 최적화

  • Published : 2010.03.31
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Abstract

Extraction characteristics of pumpkin (Cucurbita moschata Duch.) and the functional properties of the corresponding extracts were monitored by the response surface methodology (RSM). The maximum electron donating ability was found as 24.57% at the conditions of 33.13 watt microwave power, 53.67% ethanol concentration and 3.76 min extraction time. The maximum tyrosinase inhibition was 96.59% at 56.21 watt, 68.02% and 7.97 min. The SOD-like activity was 45.57% at 36.00 watt, 71.51% and 5.28 min. The total polyphenol content was 85.02% at 127.39 watt, 76.18% and 2.68 min. Based on superimposition of four dimensional RSM with respect to electron donating ability, tyrosinase inhibition, SOD-like activity and total polyphenol contents obtained under the various extraction conditions, the optimum ranges of extraction conditions were found to be microwave power of 72~144 watt, ethanol concentration of 0~38% and extraction time of 6~9 min.

늙은 호박을 최적의 추출조건에서 추출물을 얻고자 반응표면분석을 이용하여 추출조건을 최적화하였다. 중심합성 계획에 따라 요인변수로 마이크로웨이브 에너지(30~150 watt), 에탄올 농도(0~100%) 및 추출시간(1~9분)을 달리하고, 전자공여작용, tyrosinase 저해효과, SOD 유사활성 및 총 폴리페놀 함량을 종속변수로 하여 추출하였다. 추출 결과 종속변수에 따라 회귀식의 $R^2$이 각각 0.7035, 0.7748, 0.9496 및 0.9219로 나타나 SOD 유사활성과 총 폴리페놀 함량의 유의성이 인정되었다. 종속변수인 전자공여작용, tyrosinase 저해효과, SOD 유사활성 및 총 폴리페놀 함량 모두 마이크로웨이브 에너지의 영향이 가장 큰 것으로 나타났다. 네 가지 종속변수의 극대값을 얻기 위한 최적 추출조건의 범위는 4차원 반응표면분석을 superimposing하여 얻었다. 그 결과 최적 추출조건이 마이크로웨이브 에너지 72~144 watt, 에탄올 농도 0~38%, 추출시간 6~9분의 범위를 나타내었다.

Keywords

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