DOI QR코드

DOI QR Code

In vitro Effects of L-Ascorbic Acid and Acrylamide on Lymphocyte Proliferation in Young and Aged Mice

  • Published : 2010.03.31

Abstract

This study examined the effects of Acrylamide (ACR) and L-ascorbic acid (AsA) on the proliferation of splenocytes and the mitogen-stimulated lymphocyte proliferation in young (8 weeks) and aged (82 weeks) C57BL/6male mice in vitro. AsA increased splenocyte proliferation in both groups; however, this effect was higher in old mice, while the proliferation of lymphocyte was decreased except for treatment at $1\;{\mu}g/mL$ low concentration in both mice. In addition, ACR treatment resulted in decreased LPS-induced B lymphocyte proliferation and ConA-induced T lymphocyte proliferation in both groups. However, AsA increased LPS/ConA-induced lymphocyte proliferation in young groups and had no effects in old mice except at $0.5\;{\mu}g/mL$ Thus, the present data indicate that there is no difference effect of ACR and AsA on lymphocyte proliferation, whereas the effect of AsA on mitogen-induced cell proliferation was reduced in old mice. Overall, our results suggest that various immunomodulators have differing effects of lymphocytic proliferation on young versus aged mice.

Keywords

References

  1. Takeoka Y, Chen SY, Yago H, Boyd R, Suehiro S, Shultz LD, Ansari AA, Gershwin ME. 1996. The murine thymic microenvironment: changes with age. Int Arch Allergy Immunol 111: 5-12.
  2. Boehmer ED, Goral J, Faunce DE, Kovacs EJ. 2004. Age-dependent decrease in Toll-like receptor 4-mediated proinflammatory cytokine production and mitogen-activated protein kinase expression. J Leukoc Biol 75: 342-349. https://doi.org/10.1189/jlb.0803389
  3. Lopachin RM, Gavin T. 2008. Acrylamide-induced nerve terminal damage: relevance to neurotoxic and neurodegenerative mechanisms. J Agric Food Chem 56: 5994-6003. https://doi.org/10.1021/jf703745t
  4. Prockop DJ, Kivirikko KI. 1995. Collagens: molecular biology, diseases, and potentials for therapy. Annu Rev Biochem 64: 403-434. https://doi.org/10.1146/annurev.bi.64.070195.002155
  5. Otsuka E, Kato Y, Hirose S, Hagiwara H. 2000. Role of ascorbic acid in the osteoclast formation: induction of osteoclast differentiation factor with formation of the extracellular collagen matrix. Endocrinology 141: 3006-3011. https://doi.org/10.1210/en.141.8.3006
  6. Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. J Immunol Methods 65: 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  7. Taub DD, Longo DL. 2005. Insights into thymic aging and regeneration. Immunol Rev 205: 72-93. https://doi.org/10.1111/j.0105-2896.2005.00275.x
  8. Listì F, Candore G, Modica MA, Russo M, Di Lorenzo G, Esposito-Pellitteri M, Colonna-Romano G, Aquino A, Bulati M, Lio D, Franceschi C, Caruso C. 2006. A study of serum immunoglobulin levels in elderly persons that provide new insights into B cell immunosenescence. Ann N Y Acad Sci 1089: 487-495. https://doi.org/10.1196/annals.1386.013
  9. Li YH, Su H, He W, Cui LX, Hiromoto O. 2001. Differential expression analysis and cloning of murine thymic aged-related genes. Zhongguo Yi Xue Ke Xue Yuan Xue Bao 23: 158-162.
  10. Phipps SM, Berletch JB, Andrews LG, Tollefsbol TO. 2007. Aging cell culture: methods and observations. Methods Mol Biol 371: 9-19. https://doi.org/10.1007/978-1-59745-361-5_2
  11. Lai SR, Phipps SMO, Andrews LG, Tollefsbol TO. 2005. Epigenetic control of telomerase and modes of telomere maintenance in aging and abnormal systems. Front Biosci 10: 1779-1796. https://doi.org/10.2741/1661
  12. Rubin H. 1997. Cell aging in vivo and in vitro. Mech Ageing Dev 98: 1-35. https://doi.org/10.1016/S0047-6374(97)00067-5
  13. Frippiat C, Chen QM, Remacle J, Toussaint O. 2000. Cell cycle regulation in $H_2O_2$ induced premature senescence of human diploid fibroblasts and regulatory control exerted by the papilloma virus E6 and E7 proteins. Exp Gerontol 35: 733-745. https://doi.org/10.1016/S0531-5565(00)00167-4
  14. Alexander K, Yang HS, Hinds PW. 2004. Cellular senescence requires CDK5 repression of Rac1 activity. Mol Cell Biol 24: 2808-2819. https://doi.org/10.1128/MCB.24.7.2808-2819.2004
  15. Speziali E, Santiago AF, Fernandes RM, Vaz NM, Menezes JS, Faria AM. 2009. Specific immune responses but not basal functions of B and T cells are impaired in aged mice. Cell Immunol 256: 1-5. https://doi.org/10.1016/j.cellimm.2009.01.010

Cited by

  1. Effects of L-ascorbic acid on the production of pro-inflammatory and anti-inflammatory cytokines in C57BL/6 mouse splenocytes vol.30, pp.1, 2015, https://doi.org/10.7180/kmj.2015.30.1.41