Advances in Traditional Medicine

  • 제10권1호
  • /
  • Pages.21-28
  • /
  • 2010
  • /
  • 2662-4052(pISSN)
  • /
  • 2662-4060(eISSN)
경희대학교 융합한의과학연구소 (Kyung Hee Oriental Medicine Research Center)
권호 표지
DOI QR코드

DOI QR Code

Effect of various chromatographic terpenoid fractions of Luffa cylindrica seeds on in-vitro antimicrobial studies

  • Nagarajan, K. (Division of Bio-medicinal Chemistry R&D Laboratory, Department of Pharmacy, Iimt College of Medical Sciences) ;
  • Saxena, Pallavi (Department of Pharmacology, Shobit University) ;
  • Mazumder, Avijit (Department of Pharmaceutical Technology, Noida Inst. of Engg. & Tech.) ;
  • Ghosh, L.K. (Department of Pharmaceutical Technology, Jadavpur University) ;
  • Devi, G. Uma (Department of Microbiology, Periyar College of Pharmaceutical Sciences)
  • 발행 : 2010.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The objective of the present investigation is to evaluate the antimicrobial potency of the terpenoid fractions isolated from Luffa cylindrica seeds against various pathogenic microbes. The seeds were powdered and extracted with methanol in soxhlet appratus based on phytochemical screening. Three terpenoid components were isolated by column chromatography and identified by thin layer chromatography and chemical analysis which were designated as ${LCSF_4}^*$, ${LCSF_6}^*$ & ${LCSF_8}^*$ respectively. Disc diffusion method was employed to determine the antimicrobial effectiveness of test compounds I, II and III $({LCSF_4}^*,\;{LCSF_6}^*\;&\;{LCSF_8}^*)$ against 6 microbial species viz., Staphylococcus (S.) aureus, Bacillus (B.) subtilis, Escherichia (E.) coli, Pseudomonas (P.) aeruginosa, Candida (C.) albicans and Aspergillus niger. The disc was saturated with $100{\mu}l$ of each compound, allowed to dry and introduced on the upper layer of seeded agar plate. The plates were incubated overnight at $37^{\circ}C$. Microbial growth was determined by measuring the zonal inhibition diameters. Compound I showed maximum potency against gram positive S. aureus (21 mm) in comparison with standard ciprofloxacin (38 mm), whereas the same compound was completely devoid of activity against both the fungi tested. Compound II was found to be highly sensitive against both the gram negative E. coli (20 mm) and P. aeruginosa (22 mm). Compound II was found to exhibit maximum potency against the fungi C. albicans (15 mm) and A. niger (20 mm). Compound III was found to be very effective against both the gram positive S. aureus (20 mm) and B. subtilis (15 mm) respectively.

키워드

참고문헌

  1. Alper J. (1998) Effort to combat microbial resistance lags. ASM News 64, 440-441.
  2. Bauer AW, Kirby WMM, Sherris JC, Turck M. (1996) y col. Antibiotic susceptibility testing by standardized single disck method. Am. J. Clin. Pathol. 45, 493-496.
  3. Cappuccino JG, Sherman N. (1996) Microbiology-A Laboratory Manual, pp. 21-23, 4th Edn., The Benjamin Cummmings Publishing Co. Inc., California.
  4. Chopra RN, Nayar SL, Chopra IC. (2002) Glossary of Indian Medicinal Plants, pp. 156-157, 6th Reprint., National Institute of Science Communication and Information Resources (CSIR), New Delhi.
  5. Cichewicz RH, Thorpe PA. (1996) The antimicrobial properties of Chile peppers (Capsicum species) and their uses in Mayan medicine. J. Ethnopharmacol. 52, 61-70. https://doi.org/10.1016/0378-8741(96)01384-0
  6. Clark AM. (1996) Natural products as a resource for new drugs. Pharm. Res. 13, 1996.
  7. Cowan MM. (1999) Plant products as antimicrobial agents. Clin. Microbiol. Rev. 12, 564-582.
  8. De Boer HJ, Kool A, Broberg A, Mziray WR, Hedberg I, Levenfors JJ. (2005)Antifungal and antibacterial activity of some herbal remedies from Tanzania. J. Ethnopharmacol. 96, 461-469. https://doi.org/10.1016/j.jep.2004.09.035
  9. Ebner DC, Culhane JC, Winkelman TN, Haustein MD, Ditty JL, Ippoliti JT. (2008) Synthesis of novel oxazolidione antimicrobial agents. Bioorg. Med. Chem. 16, 2651-2656. https://doi.org/10.1016/j.bmc.2007.11.040
  10. Geissman TA. (1963) Flavonoid compounds, tannins, lignins and related compounds, pp. 265, Vol. 9, Elsevier., New York.
  11. Greenwood D. (2000) Antimicrobial chemotherapy, pp. 36, Oxford Univ. Press, New York.
  12. Habtemariam S, Gray AI, Waterman PG. (1993) A new antibacterial sesquiterpene from Premna oligotricha. J. Nat. Prod. 56, 140-143.
  13. Hossain SM, Easmin S, Islam SM, Mamunur R. (2004) Novel thiocyanato complexes with potent cytotoxic and antimicrobial properties. J. Pharm. Pharmacol. 56, 1519-1520. https://doi.org/10.1211/0022357044913
  14. Kubo L, Muroi H, Himejima M. (1992) Antibacterial activity of totarol and its potentiation. J. Nat. Prod. 55, 1436-1440. https://doi.org/10.1021/np50088a008
  15. Lewis WH, Elvin-Lewis MP. (1995) Medicinal plants as sources of new therapeutics. Ann. Mo. Bot. Gard. 82, 16-24. https://doi.org/10.2307/2399976
  16. Lin J, Opuku AR, Geheeb-Keller M, Hutchings AD, Terblanche SE, Jager AK, Van Staden J. (1999) Preliminary screening of some traditional Zulu medicinal plants for anti-inflammatory and antimicrobial activities. J. Ethnopharmacol. 68, 267-274. https://doi.org/10.1016/S0378-8741(99)00130-0
  17. Mendoza L, Wilkens M, Urzua A. (1997) Antimicrobial study of the resinous exudates and of diterpenoids and flavonoids isolated from some Chilean Pseudo gnaphalium (Asteraceae). J. Ethnopharmacol. 58, 85-88. https://doi.org/10.1016/S0378-8741(97)00084-6
  18. Mohamed Ali. (1998) GATE Pharmacy, pp. 298, 5th Edn., Tara Publishers., New Delhi.
  19. Nagarajan K, Mazumder A, Ghosh LK. (2006) Comparative antimicrobial evaluation studies of the extracts and isolates of leaves & bark of Wrightia tomentosa. Ancient Sci. Life. 26 (1&2), 12-18.
  20. Oyetayo FL, Oyetayo VO, Ajewole V. (2007) Phytochemical profile and antibacterial properties of the seed and leaf of the Luffa plant (Luffa cylindrica). J. Pharmacol. Toxicol. 2, 586-589. https://doi.org/10.3923/jpt.2007.586.589
  21. Paech K, Tracey MV. (1955) Modern Methods of Plant Analysis, pp. 66-79, Vol. 3, Springer-Verlag., Berlin.
  22. Parekh J, Chanda S. (2006) In-vitro antimicrobial activity of some Indian folklore medicinal plants. J. Cell Tissue Res. 6, 577-580.
  23. Rana BK, Singh UP, Taneja V. (1997) Antifungal activity and kinetics of inhibition by essential oil isolated from leaves of Aegle marmelos. J. Ethnopharmacol. 57, 29-34. https://doi.org/10.1016/S0378-8741(97)00044-5
  24. Rastogi RP, Mehrotra BN. (2004) Compendium of Indian Medicinal Plants, pp. 250, 3rd Reprint., Vol. 1, Central Drug Research Institute., Lucknow and National Institute of Science Communication and Information Resources., New Delhi.
  25. Reiner R. (1982) Detection of antibiotic activity. In: Antibiotics an Introduction, pp. 21-25, Roche Scientific Services, Switzerland.
  26. Schultes RE. (1978) The Kingdom of Plants, pp. 208, McGraw Hill Book Co., New York.
  27. Seely HW, Van Denmark PJ. (1972) Microbes in action, pp. 361, 2nd Edn., W.H. Freeman & Co., USA.
  28. Stokes EJ. (1975) Clinical Bacteriology, pp. 226, 4th Edn., Edward Arnold Ltd., London.
  29. Strohl WR. (1997) Biotechnology of Antibiotics, pp. 9, 2nd Edn., Vol. 82, Marcel Dekker Inc., New York.
  30. Taylor RSL, Edel F, Manandhar NP, Towers GHN. (1996) Antimicrobial activities of Southern Nepalese Medicinal Plants. J. Ethnopharmacol. 50, 97-102. https://doi.org/10.1016/0378-8741(95)01335-0

피인용 문헌

  1. Delile (Acanthaceae) vol.2018, pp.1741-4288, 2018, https://doi.org/10.1155/2018/2047896