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Optimization of Enzymatic Pretreatment for the Production of Fermented Ginseng using Leaves, Stems and Roots of Ginseng

  • Cho, Kyung-Lae (Department of Food and Biotechnology and Center for Food Function and Safety, Hoseo University) ;
  • Woo, Hye-Jin (Department of Food and Biotechnology and Center for Food Function and Safety, Hoseo University) ;
  • Lee, In-Sook (Gimpo-City Agricultural Technology Center) ;
  • Lee, Jun-Won (Gyeonggi Agricultural Research and Extension Services) ;
  • Cho, Young-Cheol (Gyeonggi Agricultural Research and Extension Services) ;
  • Lee, Il-Nam (R&D Center, Nu-Pharm, Co., Ltd.) ;
  • Chae, Hee-Jeong (Department of Food and Biotechnology and Center for Food Function and Safety, Hoseo University)
  • Published : 2010.03.31

Abstract

This study sought to optimize the extraction and enzymatic treatment conditions of Panax ginseng leaves, stems, and roots for the production of fermented ginseng. The optimization enhanced the extraction of total saccharide, a nutrient and growth-activating factor for Lactobacillus bacteria. The hydrolysis of ginseng leaves, stems, and roots was tested with eight enzymes (Pentopan, Promozyme, Celluclast, Ultraflo, Pectinex, Ceremix, Viscozyme, and Tunicase). The enzymatic hydrolysis conditions were statistically optimized by the experimental design. Optimal particle size of ginseng raw material was <0.15 mm, and optimal hydrolysis occurred at a pH of 5.0-5.5, a reaction temperature of 55-$60^{\circ}C$, a Ceremix concentration of 1%, and a reaction time of 2 hr. Ceremix produced the highest dry matter yield and total saccharide extraction. Ginseng leaves were found to be the most suitable raw material for the production of fermented ginseng because they have higher carbohydrate and crude saponin contents than ginseng roots.

Keywords

References

  1. Elma ZT, Ilian EZ, Christina IH. Effect of ginsenoside Rg1 on insulin binding in mice liver and brain membrane. Phytother Res. 5: 46-48 (1991) https://doi.org/10.1002/ptr.2650050114
  2. Attle AS, Wu JA, Yuan CS. Ginseng pharmacology: multiple constituents and multiple action. Biochem Pharmacol. 58: 1685-1693 (1999) https://doi.org/10.1016/S0006-2952(99)00212-9
  3. Im KS, Chung HY, Park SH, Je NK. Anticancer effect of the hydrolyzed monogluco-ginsenoside of total saponin from ginseng leaf. Korean J Ginseng Sci. 19: 291-294 (1995)
  4. Kim MJ, Jung NP. The effect of ginseng saponin on the mouse immune system. Korean J Ginseng Sci. 11: 130-135 (1987)
  5. Kang SY, Kim ND. The antihypertensive effect of red ginseng saponin and the endothelium derived vascular relaxation. Korean J Ginseng Sci. 16: 175-182 (1992)
  6. Wu JY, Gardner BH, Murphy CI, Seals JR, Kensil CR, Recchia J, Beltz GA, Newman GW, Newman MJ. Saponin adjuvant enhancement of antigen-specific immuneresponses to an experimental HIV-1 vaccine. J Immunol. 148: 1519-1525 (1992)
  7. Bae EA, Cho MK, Park EK, Park SY, Shin HY, Kim DH. Metabolism of ginsenoside Rc by human intestinal bacteria and its related antiallergic activity. Biol Pharm Bull. 25: 743- 747 (2002) https://doi.org/10.1248/bpb.25.743
  8. Park EK, Cho MK, Han MJ, Kim DH. Antiallergic and antiinflammatory activities of ginsenoside $Rh_1$. Int Arch Allergy Immunol. 133: 113-120 (2004) https://doi.org/10.1159/000076383
  9. Shibata S, Tanaka O, Soma K, Iita Y, Ando Y, Nakamura H. Studies on saponins and sapogenins of ginseng, the structure of panaxatriol. Tetra Lett. 3: 207-213 (1965)
  10. Shibata S. Some chemical studies on ginseng. In: Proceedings of International Ginseng Symposium, The Korean Society of Ginseng, Seoul, 1: 69-76 (1974)
  11. Namba T. The encyclopedia of wakan-yaku(traditional sino- Japanese medicines) with color pictures. Hoikusha, Osaka, Japan 1: 50-51 (1993)
  12. Choi YE, Jeong JH. Recent progress of ginseng biotechnology and progress toward food application. J Food Ind Nutr. 8: 24-29 (2003)
  13. Lee BY. Status of Korean ginseng industry and development of new ginseng products. J Food Ind Nutr. 8: 1-9 (2003)
  14. Park S, Cho YJ, Pyee J, Hong HD. Meta-analysis of studies and patents on Korean ginseng in recent 5 years in Korea and prospective needs. J Ginseng Res. 30: 212-219 (2006) https://doi.org/10.5142/JGR.2006.30.4.212
  15. Kitagawa I, Yoshikawa M, Yoshihara M, Hayashi T, Taniyama. Chemical studies on crude drug procession. I, on the constituents of ginseng radix rubura (I). Yakugaku Zasshi 103: 612- 622 (1983) https://doi.org/10.1248/yakushi1947.103.6_612
  16. Park JH. Sun ginseng-a new processed ginseng with fortified activity. J Food Ind Nutr. 9: 23-27 (2004)
  17. Han BH, Park MH, Han YN, Woo LK, Sankawa U, Yahara S, Tanaka O. Degradation of ginseng saponins under mild acidic conditions. Planta Medica. 44: 146-149 (1982) https://doi.org/10.1055/s-2007-971425
  18. Bae EA, Han MJ, Kim EJ, Kim DH. Transformation of ginseng saponins to ginsenoside $Rh_2$ by acids and human intestinal bacteria and biological activities of their transformants. Arch Pharm Res. 27: 61-67 (2004) https://doi.org/10.1007/BF02980048
  19. Chen Y, Nose M, Ogihara Y. Alkaline cleavage of ginsenosides. Chem Pharm Bull. 35: 1653-1655 (1987) https://doi.org/10.1248/cpb.35.1653
  20. Kim SD, Do JH, Lee JC. Effect of red ginseng residue on various enzyme production of alcohol fermentation koji. Korean J Ginseng Sci. 6: 131-137 (1982)
  21. Bae EA, Park SY, Kim DH. Constitutive ${\beta}$-glucosidases hydrolyzing ginsenoside $Rb_1$ and $Rb_2$ from human intestinal bacteria. Biol Pharm Bull. 23: 1481-1485 (2000) https://doi.org/10.1248/bpb.23.1481
  22. Park SY, Bae EA, Sung JH, Lee SK, Kim DH. Purification and characterization of ginsenoside $Rb_1$-metabolizing ${\beta}$-glucosidase from fusobacterium K-60, a human intestinal anaerobic bacterium. Biosci Biotechnol Biochem. 65: 1163-1169 (2001) https://doi.org/10.1271/bbb.65.1163
  23. Yu H, Liu H, Zhang C, Tan D, Lu M, Jin F. Purification and characterization of gypenoside-${\alpha}$-L-rhamnosidase hydrolyzing gypenoside-5 into ginsenoside Rd. Process Biochem. 39: 861-867 (2004) https://doi.org/10.1016/S0032-9592(03)00196-1
  24. Ko SR, Suzuki Y, Choi KJ, Kim YH. Enzymatic preparation of genuine prosapogenin, 20(S)-ginsenoside Rh1, from ginsenosides Re and $Rg_1$. Biosci Biotechnol Biochem. 64: 2739- 2743 (2000) https://doi.org/10.1271/bbb.64.2739
  25. Hasegawa H, Sung JH, Matssumiya S, Uchiyama M. Main ginseng saponin metabolites formed by intestinal bacteria. Planta Med. 62: 453-457 (1996) https://doi.org/10.1055/s-2006-957938
  26. Hasegawa H, Sung JH, Benno Y. Role of human intestinal prevotella oris in hydrolyzing ginseng saponins. Planta Med. 63: 436-440 (1997) https://doi.org/10.1055/s-2006-957729
  27. Suzuk, Y, Ko SR, Choi KJ, Uchida K, Lee YG, Kim YH. Enzymatic glycosylation and hydrolysis of ginseng saponins. In: Proceedings of International Ginseng Symposium, The Korean Society of Ginseng, Seoul, 7: 373-374 (1988)
  28. Choi KJ, Kim MW, Kim DH. Fatty acid compositions of the various parts of ginseng plant. Korean J Food Nutr. 12: 357- 363 (1983)
  29. Park H, Park HS, Hong JU. Effect of high temperature Panax ginseng leaf. J Korean Agric Chem Soc. 29: 336-371 (1986)
  30. Zhang S, Takeda T, Zhu T, Yao X, Tanaka Y, Okihara Y. A new minor saponin from the leaves of Panax ginseng. Planta Med. 56: 298-301 (1990) https://doi.org/10.1055/s-2006-960963
  31. Lee JW, Do JH. Antioxidative activity of ethanol extraction fraction from the Korean red tail ginseng. Korean J Food Sci Technol. 33: 497-500 (2001)
  32. Zhang S, Yao X, Chen Y, Cui C, Tezuka T, Kikuchi T. Ginsenoside la, a novel saponin from the leaves of Panax ginseng. Chem Pharm Bull. 37: 1966-1968 (2001)
  33. Chang HK. Effect of processing methods on the saponin contents of Panax ginseng leaf-tea. Korean J Food Nutr. 16: 46- 53 (2003)
  34. KFIA. Food code. Korea Association Food Industry, Seoul, p 585-624 (2006)
  35. Chae SG, Kang KS, Ma SJ, Bang KU, Oh MH, Oh SH. Analysis of Food. Jigu Publishing, Seoul, p 270-275 (2000)
  36. Dubois M, Gillers KA, Hamilton JK, Rebers PA, Smith J. Colorimetric method for determination of sugar and related substance. Anal Chem. 28: 350-352 (1956) https://doi.org/10.1021/ac60111a017
  37. Kim HJ, Jo JS, Nam SH, Park SH, Mhee KC. Free sugar distribution in ginseng plant and change of it's content in the root with dehydration. Korean J Ginseng Sci. 7: 44-50 (1987)
  38. Tark K, Cho K, Park KH, Son S M, Chae HJ. Optimization of extraction conditions for polysaccharide using red ginseng marc. J Ginseng Res. 33: 337-342 (2009) https://doi.org/10.5142/JGR.2009.33.4.337
  39. Cho CW, Kim SW, Rho J, Rhee YK, Kim K. Extraction characteristic of saponin and acidic polysaccharide based in the red ginseng particle size. J Ginseng Res. 32: 179-186 (2008) https://doi.org/10.5142/JGR.2008.32.3.179
  40. Lee JW, Do JH. Extraction condition of acidic polysaccharide from Korean red ginseng marc. J Korea Ginseng Res. 26: 202-205 (2002) https://doi.org/10.5142/JGR.2002.26.4.202

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