Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
권호 표지
DOI QR코드

DOI QR Code

Comparison of Bifidobacteria Selective Media for the Detection of Bifidobacteria in Korean Commercial Fermented Milk Products

  • Published : 2010.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to compare the efficacy and selectivity of TOS and BS media for enumeration of bifidobacteria in commercial fermented milk products. First, bifidobacteria was isolated from 20 fermented milk products, and all isolated bifidobacteria were identified by genomic technology as Bifidobacterium lactis. The two media significantly differed from each other with regard to the recovery of B. lactis, that is, the recovery of this organism was as much as 6 logs lower on BS medium than on TOS. When the concentration of BS solution (mixture of paromomycin sulfate, neomycin, sodium propionate, and lithium chloride) used in BS medium was reduced to 50% (BS50), a relatively high percentage recovery of bifidobacteria from pure cultures was achieved. Susceptibility tests to antibiotics and tests for selective agents for the isolated bifidobacteria and lactic acid bacteria were conducted. The BS solution inhibited some lactic acid bacteria and Bifidobacterium species, while mupirocin (MU) suppressed the growth of all tested lactic acid bacteria but not Bifidobacterium. As compared with BS50 medium, TOS with or without MU showed good bifidobacteria recovery and readily distinguishable colonies; in particular, TOS supplemented with MU had a high selectivity for bifidobacteria. In conclusion, all results suggested that TOS medium with or without MU was found to be suitable for selective enumeration of bifidobacteria from mixed cultures in fermented milk, and better in that capacity than BS medium.

Keywords

References

  1. Biavati, B. and Mattarelli, P. (2005) The family Bifidobacteriaece. In: The prokaryotes: a handbook on the biology of bacteria, 3rd ed. Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K.H. and Stakebrandt, E. (eds.), Springer, NY, Vol 3, pp 333.
  2. Camashella, P., Mignot, O., Pirovano, F., and Sozzi, T. (1998) Methods for differentiated enumeration of mixed cultures of thermophilic lactic acid bacteria and bifidobateria by using only one culture medium. Lait. 78, 461-467. https://doi.org/10.1051/lait:1998444
  3. Collado, M. C., Moreno, Y., Hernandez, E., Cobo, J. M., and Hernandez, M. (2005) In vitro viability of Bifidobacterium strains isolated from commercial dairy products exposed to human gastrointestinal conditions. Food Sci. Tech. Int. 11, 307-314. https://doi.org/10.1177/1082013205056559
  4. Hartemink, R., Kok, B. J., Weenk, G. H., and Rombouts, F. M. (1996) Raffinose-Bifidobacterium (RB) agar, a new selective medium for bifidobacteria. J. Microbiological Methods. 27, 33-43. https://doi.org/10.1016/0167-7012(96)00926-8
  5. IDF (2007) Selective enumeration of bifidobacteria in dairy products: development of a standard method. Bulletin of theInternational Dairy Federation 411/2007.
  6. Ji, G. E., Lee, S. K., and Kim, I. H. (1994) Improved selective medium for isolation and enumeration of Bifidobacterium sp. Korean J. Food Sci. Technol. 26, 526-531.
  7. Lee, J. J., Shin, M. S., Na, S. H., Bae, H. S., and Baek, Y. J. (1994) Selective media containing antibiotics for counting Bifidobacteria. Kor. J. Appl. Micobiol. Biotechnol. 22, 309-315.
  8. Lim, K. S., Huh, C. S., Baek, Y. J., and Kim, H. U. (1995) A selective enumeration medium for bifidobacteria in fermented dairy products. J. Dairy Sci. 78, 2108-2112. https://doi.org/10.3168/jds.S0022-0302(95)76837-0
  9. Martineau, B. (1999) Comparison of four media for the selection of bifidobacteria in dog fecal samples. Anaerobe. 5, 123-127. https://doi.org/10.1006/anae.1999.0238
  10. Nebra, Y. and Blanch, A. R. (1999) A new selective medium for Bifidobacterium spp. Appl. Environ. Microbiol. 65, 5173-5176.
  11. Payne, J. F., Morris, A. E. J., and Beers, P. (1999) Evaluation of selective media for the enumeration of Bifidobacteriumsp. in milk. J. Appl. Microbiol. 86, 353-358. https://doi.org/10.1046/j.1365-2672.1999.00671.x
  12. Roy, D. (2001) Media for the isolation and enumeration of bifidobacteria in dairy products. Int. J. Food Microbiol. 69,167-182. https://doi.org/10.1016/S0168-1605(01)00496-2
  13. Shah, N. P. (2007) Functional cultures and health benefits. Int. Dairy J. 17, 1262-1277. https://doi.org/10.1016/j.idairyj.2007.01.014
  14. Shin, M. S., Lee, J. J., Seo, I. Y., Na, S. H., and Baek, Y. J. (1994) Selective medium for the isolation and counting of bifidobacteria in dairy products. Kor. J. Appl. Microbiol. Biotechnol. 22, 210-216.
  15. Silvi, S., Rumney, C. J., and Rowland, I. R. (1996) An assessment of three selective media for bifidobacteria infaeces. J. Appl. Bacteriol. 81, 561-564.
  16. Tanaka, R. and Mutai, M. (1980) Improved medium for selective isolation and enumeration of Bifidobacterium.Appl. Environ. Microbiol. 40, 866-869.
  17. Temmerman, R., Pot, B., Huys, G., and Swings, J. (2003) Identification and antibiotic susceptibility of bacterial isolates from probiotic products. Int. J. Food Microbiol. 81, 1-10. https://doi.org/10.1016/S0168-1605(02)00162-9
  18. Thitaram, S. N., Siragusa, G. R., and Hinton, Jr, A. (2005) Bifidobacterium-selective isolation and enumeration from chicken caeca by a modified oligosaccharide antibioticselectiveagar medium. Lett. Appl. Microbiol. 41, 355-360. https://doi.org/10.1111/j.1472-765X.2005.01765.x
  19. Van de Casteele, S., Vanheuerzwijn, T., Ruyssen, T., Van Assche, P., Swings, J., and Huys, G. (2006) Evaluation of culture media for selective enumeration of probiotic strains of lactobacilli and bifidobacteria in combination with yoghurt or cheese starters. Int. Dairy J. 16, 1470-1476. https://doi.org/10.1016/j.idairyj.2005.12.002
  20. Vasiljevic, T. and Shah, N. P. (2008) Probiotic-from Metchnikoff to bioactives. Int. Dairy J. 18, 714-728. https://doi.org/10.1016/j.idairyj.2008.03.004
  21. Vlkova, E., Rada, V., and Trojanova, I. (2004) Enumeration, isolation, and identification of bifidobacteria from dairyproducts. Acta Agriculturae Slovenica. 84, 31-36.

Cited by

  1. Concentration of xylooligosaccharides with a low degree of polymerization using membranes and their effect on bacterial fermentation vol.15, pp.1, 2021, https://doi.org/10.1002/bbb.2145