Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지

Antioxidant and Neuronal Cell Protective Effects of an Extract of Houttuynia cordata Thunb (a Culinary Herb)

어성초 추출물의 항산화 및 신경세포 보호효과

  • Jeong, Hee-Rok (Department of Food Science & Technology, and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Kwak, Ji-Hyun (Department of Food Science & Technology, and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Kim, Ji-Hye (Department of Food Science & Technology, and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Choi, Gwi-Nam (Department of Food Science & Technology, and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Jeong, Chang-Ho (Department of Food Science & Technology, Kyunghee University) ;
  • Heo, Ho-Jin (Department of Food Science & Technology, and Institute of Agriculture & Life Science, Gyeongsang National University)
  • 정희록 (경상대학교 농업생명과학대학 식품공학과 및 농업생명과학연구원) ;
  • 곽지현 (경상대학교 농업생명과학대학 식품공학과 및 농업생명과학연구원) ;
  • 김지혜 (경상대학교 농업생명과학대학 식품공학과 및 농업생명과학연구원) ;
  • 최귀남 (경상대학교 농업생명과학대학 식품공학과 및 농업생명과학연구원) ;
  • 정창호 (경희대학교 생명과학대학 식품공학과) ;
  • 허호진 (경상대학교 농업생명과학대학 식품공학과 및 농업생명과학연구원)
  • Received : 2010.04.30
  • Accepted : 2010.09.03
  • Published : 2010.10.30
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Abstract

The in vitro antioxidant activities and neuronal cell protective effects of 60% (w/v) methanolic extract from Houttuynia cordata were investigated. The contents of total phenolics and quercitrin in the extract were 17.71 mg/g and 75.80 ${\mu}g$/g, respectively. DPPH and ABTS radical-scavenging activities were 87.79% and 99.27%, respectively, when the extract was tested at 5 mg/ml. The FRAP (ferric reducing/antioxidant power) assay showed a dose-dependent increse in activity. In a cell viability assay using MTT, the extract protected against $H_2O_2$-induced neurotoxicity. Lactate dehydrogenase (LDH) leakage was also inhibited by the extract, as was lipid peroxidation as shown using the mouse brain homogenate test. These data indicate that a 60% (w/v) methanolic extract of Houttuynia cordata has in vitro antioxidant activities, and ingestion there of may reduce the risk of developing neurodegenerative disorders.

본 연구에서는 예비실험결과 높은 총 페놀 화합물 함량(17.71 mg/g)을 나타낸 어성초 60% 메탄올 추출물의 항산화 효과 및 신경세포 보호효과를 알아보기 위해 다양한 연구를 진행하였다. 어성초의 DPPH와 ABTS radical 소거 활성 및 FRAP assay결과 농도 의존적인 경향이 나타났으며, 높은 항산화 활성을 보여주었다. MTT, LDH assay를 통한 신경세포 보호효과를 측정한 결과 MTT 실험에서는 어성초 60% 메탄올 추출물의 모든 농도에서 positive control로서의 vitamin C와 유사한 세포 생존율을 나타냈고, LDH 실험에서는 추출물에 의한 농도 의존적인 효소 방출량 감소가 관찰되었다. 또한 LDH 실험에서 나타난 신경세포막 파괴의 원인을 확인하고자 뇌 조직을 이용한 지질의 과산화 억제 활성을 측정한 결과 농도 의존적으로 지질의 과산화가 억제 되는 것으로 나타나 지질의 과산화는 신경세포의 파괴를 유발시킬 수 있다는 가능성을 확인하였다. 본 연구결과를 종합해 볼 때 quercitrin 및 다양한 페놀성 화합물을 함유한 어성초 60% 메탄올 추출물은 항산화 및 산화적 스트레스로부터 나타나는 지질의 과산화로 인한 신경세포 보호효과를 나타내어 퇴행성 신경질환 등을 예방할 수 있는 기능성 식품 소재로서의 활용 가치가 높다고 판단된다.

Keywords

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