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Antioxidative and Antigenotoxic Effect of Omija (Schizandra chinensis B.) Extracted with Various Solvents

다양한 용매에 의해 추출된 오미자의 항산화능과 항유전독성 효과

  • Kim, Min-Jung (Dept. of Food and Nutrition, Kyungnam University) ;
  • Park, Eun-Ju (Dept. of Food and Nutrition, Kyungnam University)
  • 김민정 (경남대학교 식품영양학과) ;
  • 박은주 (경남대학교 식품영양학과)
  • Published : 2010.04.30

Abstract

The purpose of this study was to evaluate antioxidant and antigenotoxic effects of Omija (Schizandra chinensis B.) extracted with various solvents (acetone, ethanol, and methanol). The total polyphenol content (TPC) of methanol extract (ME), ethanol extract (EE) and acetone extract (AE) from Omija were 1183.3, 1009.4, and 747.3 mg/100 g (garlic acid equivalents: GAE), respectively. Antioxidant effects of the Omija extracts was measured by DPPH radical-scavenging activity (RSA) and superoxide dismutase (SOD)-like activity. The $IC_{50}$ for DPPH RSA was in the order of EE $(1411.1\;{\mu}g/mL)$=AE $(1462.0\;{\mu}g/mL)$>ME $(1585.0\;{\mu}g/mL)$. The $IC_{50}$ for SOD-like activities was the highest in ME $(905.7\;{\mu}g/mL)$=EE $(970.3\;{\mu}g/mL)$>AE $(1579.4\;{\mu}g/mL)$. The antigenotoxic effect of Omija on DNA damage induced by $H_2O_2$ in human leukocytes was evaluated by comet assay. $H_2O_2$ induced DNA damage was effectively protected by all of the Omija extracts. Aectone extract of Omija showed the highest antigenotoxic effect ($IC_{50}$ value of AE is $14.6\;{\mu}g/mL$) followed by EE, and ME (21.4 and $34.4\;{\mu}g/mL$), respectively. As a result, we propose that Omija (Schizandra chinensis B.) can serve as a new natural source enriched with potent antioxidant and antigenotoxic agents.

오미자 5 g에 100 mL의 세 가지 용매(아세톤, 에탄올, 메탄올)를 각각 가하여 추출하여 농축한 다음, DMSO 용매에 녹여 항산화활성과 항유전독성을 조사하였다. 그 결과 총페놀 함량에서 메탄올 추출물이 1183.3 mg/100 g으로 가장 높았다. 항산화활성을 50, 100, 250, 500, $1000\;{\mu}g/mL$ 농도에서 측정하였을 때 DPPH radical 소거능과 SOD 유사활성은 각각 농도에 의존하여 결과 값이 향상되었으며, DPPH radical 소거능에서 높은 $IC_{50}$값을 나타낸 것은 에탄올 추출물로 $1411.1\;{\mu}g/mL$을 나타내었고, SOD 유사활성에서 $IC_{50}$값은 오미자 메탄올 추출물과 에탄올 추출물이 $905.7\;{\mu}g/mL$, $970.3\;{\mu}g/mL$ 순으로 비슷한 활성을 나타내었다. 또한 comet assay에서는 오미자 추출물을 1, 5, 10, $50\;{\mu}g/mL$의 농도로 백혈구에 처리한 후 $H_2O_2\;200\;{\mu}M$의 농도로 처리하여 DNA 손상을 유도한 결과 손상된 $50\;{\mu}g/mL$ 농도에서 DNA tail 부분의 DNA 함량을 측정한 % tail DNA inhibition이 각각 AE(73.1%), ME(63.7%), EE(62.8%)로 $H_2O_2$ 처리 양성 대조구에 비해 유의적으로 증가하였다. 이는 오미자에 함유되어 있는 페놀 화합물 외에 lignan 화합물을 포함한 여러 가지 다른 생리활성 물질들의 항산화력으로 인해 DPPH radical 소거능과 SOD 유사활성, 항유전독성 활성이 상호 의존적이 지 않음을 나타내고 있다고 사료된다. 따라서 오미자의 항산화 활성과 항유전독성의 메카니즘적 상호관계를 규명하는 연구가 향후 수행되어져야 할 것이다. 이상의 결과에서 오미자의 용매에 따른 추출물들은 각각 항산화 활성과 항 유전독성이 있음을 알 수 있었고, 따라서 본 연구에서는 오미자의 천연 생리활성 물질로서의 활용 가능성을 규명할 수 있었다.

Keywords

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