Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidant and Anticancer Activities of Methanol Extracts Prepared from Different Parts of Jangseong Daebong Persimmon (Diospyros kaki cv. Hachiya)

장성 대봉감의 부위별 메탄올 추출물의 항산화 활성 및 항암 활성

  • Jo, Young-Hong (Dept. of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Ji-Won (Dept. of Food Science and Biotechnology, Kyungnam University) ;
  • Lee, Jeung-Min (Dept. of Food Science and Biotechnology, Kyungnam University) ;
  • Ahn, Gwang-Hwan (Sweet Persimmon Research Institute, Gyungsangnamdo Agricultural Research & Extension Services) ;
  • Park, Hae-Ryong (Dept. of Food Science and Biotechnology, Kyungnam University) ;
  • Lee, Seung-Cheol (Dept. of Food Science and Biotechnology, Kyungnam University)
  • 조영홍 (경남대학교 식품생명학과) ;
  • 박지원 (경남대학교 식품생명학과) ;
  • 이정민 (경남대학교 식품생명학과) ;
  • 안광환 (경남농업기술원 단감연구소) ;
  • 박해룡 (경남대학교 식품생명학과) ;
  • 이승철 (경남대학교 식품생명학과)
  • Published : 2010.04.30
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Abstract

The antioxidant activity of methanol extracts from five different parts (flesh, peel, core, seed, calyx) of Jangseong Daebong persimmon (Diospyros kaki cv. Hachiya) were evaluated by determining total phenol content (TPC), DPPH radical scavenging activity (RSA), ABTS RSA, and reducing power (RP). The flesh extract gave the highest yield (92.93%) while the lowest yield was obtained from the seed (5.17%). The seed extract showed the highest total phenolic content ($76.47\pm0.009$ mg GAE/g extract), DPPH RSA ($IC_{50}=52.05\pm1.61\;{\mu}g/mL$), ABTS RSA ($IC_{50}=30.94\pm0.41\;{\mu}g/mL$) and RP ($IC_{50}=87.94\pm0.37\;{\mu}g/mL$). In addition, the calyx extract also showed high antioxidant activity. On the other hand, the core extract gave the lowest TPC and all antioxidant assays. In particular, HT-29 cells showed extensive cell death when treated with $500\;{\mu}g/mL$ of calyx extracts. Thus, these results suggest that methanolic extracts of Jangseong Daebong persimmon seed and calyx may serve as a potential source of natural antioxidant for food and nutraceutical application.

장성 대봉감을 부위별(과육, 껍질, 심, 씨, 꼭지)로 세분하여 메탄올 추출물을 제조한 후 항산화 활성과 항암 활성을 조사하였다. 총 페놀 함량은 씨>꼭지>껍질>과육>심의 순위였으며, 항산화 활성에 관해 분석한 항목인 DPPH 라디칼 소거능, ABTS 라디칼 소거능, 환원력은 모든 경우에서 씨>꼭지>과육>껍질>심의 순위로 나타났다. 인간 유래 대장암 세포주에 대한 대봉감 추출물의 항암 활성은 꼭지>씨>껍질>심>과육의 순서로 측정되었으며, 꼭지 부위 추출물은 농도의존적으로 항암 활성을 보였다. 이상의 결과로 장성 대봉감의 씨와 꼭지에서 항산화 활성과 항암 활성이 확인되어 이들 부산물을 유용하게 이용할 필요가 있을 것으로 생각된다.

Keywords

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