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Discrimination of Korean Cattle (Hanwoo) with Imported Beef from USA Based on the SNP Markers

  • Shim, Jung-Mi (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Seo, Dong-Won (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Seo, Seong-Won (Department of Animal Biosystem Sciences, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Jong-Joo (School of Biotechnology, Yeungnam University) ;
  • Min, Dong-Myung (Experiment & Research Institute, NAQS, MIFAFF) ;
  • Kim, Ik-Chul (Experiment & Research Institute, NAQS, MIFAFF) ;
  • Jeon, Jin-Tae (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, Jun-Heon (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University)
  • Received : 2010.06.04
  • Accepted : 2010.11.03
  • Published : 2010.12.31

Abstract

Due to the large amount of beef imported from the USA to Korea, Korean consumers have become increasingly interested in the country of origin since it can affect market prices. Previously, Bos indicus and Bos taurus-specific markers were developed for the purpose of cattle breed identification, specifically discrimination of Australian beef. In this study, six SNP markers derived from Illumina 50K bovine SNP chip data were used for the discrimination between Korean cattle (Hanwoo) and imported beef from USA. PCR-RFLP genotyping methods were also developed, which indicates that these markers can be applied relatively easily compared to other markers. Taking into account a discrimination rate of 55% based on MC1R marker between Hanwoo and imported beef from USA, two additional markers, SNPs 23803 and 34776, were ideal and resulted in probability of identification of 0.942 and probability of misjudgment of 0.03. Therefore, the markers developed in this study can greatly contribute to the correct discrimination between beef from USA and Hanwoo beef.

Keywords

References

  1. Adalsteinsson, S., Bjarnadottir, S., Vage, D. I., and Jonmundsson, J. V. (1995) Brown coat color in Icelandic cattle produced by the loci extension and Agouti. J. Hered. 86, 395-398.
  2. Gan, H. Y., Li, J. B., Wang, H. M., Gao, Y. D., Liu, W. H., Li, J. P., and Zhong, J. F. (2007) Allele frequencies of TYR and MC1R in Chinese native cattle. J. Anim. Sci. 78, 484-488. https://doi.org/10.1111/j.1740-0929.2007.00466.x
  3. Guibert, S., Girardot, M., Leveziel, H., Julien, R., and Qulmouden, A. (2004) Pheomelanin coat colour dilution in French cattle breeds is not correlated with the TYR, TYRP1 and DCT transcription levels. Pigment Cell Res. 17, 337-345. https://doi.org/10.1111/j.1600-0749.2004.00152.x
  4. Hearing, V. J. and Tsukamoto, K. (1991) Enzymatic control of pigmentation in mammals. FASEB J. 5, 2902-2909.
  5. Kim, T. H., Yoon, D. H., Park, E. W., Lee, H. Y., Oh, S. J., Cheong, I. C., Thak, T. Y., Kim, K. N., and Han, J. Y. 2000. A study on genotype frequencies of the bovine melanocortin recepter 1 (MC1R) in cattle breeds. J. Anim. Sci. Technol. 42, 735-744.
  6. Klungland, H., Vage, D. I., Gomez-Raya, L., Adalsteinsson, S., and Lien, S. (1995) The role of melanocyte-stimulating hormone (MSH) receptor in bovine coat color determination. Mamm. Genome 6, 636-639. https://doi.org/10.1007/BF00352371
  7. Mohanty, T. R., Seo, K. S., Park, K. M., Choi, T. J., Choe, H. S., Baik, D. H., and Hwang, I. H. (2008) Molecular variation in pigmentation genes contributing to coat colour in native Korean Hanwoo cattle. Anim. Genet. 39, 550-553. https://doi.org/10.1111/j.1365-2052.2008.01746.x
  8. Sasazaki, S., Itoh, K., Arimitsu, S., Imada, T., Takasuga, A., Nagaishi, H., Takano, S., Mannen, H., and Tsuji, S. (2004) Development of breed identification markers derived from AFLP in beef cattle. Meat Sci. 67, 275-280. https://doi.org/10.1016/j.meatsci.2003.10.016
  9. Sasazaki, S., Mutohh, H., Tsurifune, K., and Mannen, H. (2007) Development of DNA markers for discrimination between domestic and imported beef. Meat Sci. 77, 161-166. https://doi.org/10.1016/j.meatsci.2007.02.024
  10. Yoon, D., Kwon, Y. S., Lee, K. Y., Jung, W. Y., Sasazaki, S., Mannen, H., Jeon, J. T., and Lee, J. H. (2008) Discrimination of Korean cattle (Hanwoo) using DNA markers derived from SNPs in bovine mitochondrial and SRY genes. Asian-Australas. J. Anim. Sci. 21, 25-28. https://doi.org/10.5713/ajas.2008.70209

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