Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Properties and Antioxidative Activities of Phenolic Acid Concentrates of Rice Bran

미강 페놀산 농축물의 특성 및 항산화 활성

  • Received : 2010.04.23
  • Accepted : 2010.05.31
  • Published : 2010.10.31
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Abstract

This study investigated the properties and antioxidative activities of phenolic acid concentrates of rice bran. Rice bran contains bioactive substances such as phenolic compounds, which can provide health benefits as natural antioxidants. This study examined how levels of phenolic acids can be obtained efficiently through various extraction methods. The extractions of defatted rice bran were followed by using ethylacetate (RBE-I), ethylacetate after alkaline hydrolysis (RBE-II), and 80% methanol (RBE-III). For all extracts, yields (%), total polyphenol contents (TPC), various phenolic acids and antioxidative activities were estimated. RBE-II had the highest total polyphenol contents (526.72 mg/100 g rice bran) and showed high antioxidative activity (74.7%). To concentrate the phenolic acids, RBE-II was passed through Sep-pak $C_{18}$ Vac cartridge and F1-RBE-II was collected by the elution of 50% methanol. The total phenolic content of F1-RBE-II (736.8 mg/100 g rice bran) was higher than that of RBE-II (367.1 mg/100 g rice bran), and the ratios of ferulic acid (73%) and sinapic acid (14%) increased. As RBE-II was analysed by HPLC, 6 different phenolic acids were found via chromatography, whereas F1-RBE-II showed 5 different peaks and the major phenolic acid was identified as ferulic acid. The ABTS radical scavenging activity of F1-RBE-II was the highest among the rice bran extracts. In a ${\beta}$-carotene-linoleic acid model system, linoleic acid oxidation was reduced by F1-RBE-II (73%) and RBE-II (35%).

본 연구에서는 현미의 미강으로부터 생리활성이 있는 페놀산물질을 얻기 위해 다양한 추출법을 시도하였고, 얻어진 미강 페놀산 농축물의 성분 분석 및 항산화 활성에 대해 조사하였다. 미강 추출물 중 알칼리 가수분해를 시행한 다음 에틸아세테이트로 분획하여 얻은 RBE-II의 총 폴리페놀 함량과 항산화 활성이 가장 높은 것으로 조사되었고, 페놀산 성분의 농축 및 정제를 위해 Sep-pak $C_{18}$ Vac cartridge를 통과시켜 미강 페놀산 농축물(F1-RBE-II)을 얻었다. HPLC 분석을 통해 얻어진 페놀산 성분 중 ferulic acid가 가장 많았고, p-coumaric acid, sinapic acid, vanillic acid, 그리고 syringic acid가 존재하였으며, 그 구성성분이 주로 hydroxycinnamic acid 계열에 속한다는 것을 알 수 있었다. DPPH radical과 ABTS radical 소거능에서 미강 페놀산 농축물(F1-RBEII)는 강력한 항산화 활성을 나타냈으며, ${\beta}$-CLMS을 이용한 지질산화 유도반응에서도 우수한 peroxy radical 저해능을 발휘하여, 합성 항산화제인 BHA의 활성과 비교했을 때 높은 과산화 지질억제력을 보였다. 본 연구를 통해 미강 페놀산 농축물의 성분 및 강한 항산화 능력이 확인됨으로천연 항산화제 및 지방 산화 방지제로 개발하기에 부족함이 없는 것으로 사료된다.

Keywords

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