Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Effect of Resistant Starch on Body Weight and Defecation in Loperamide-induced Constipated Rats

저항전분 투여가 loperamide 유도 변비 쥐의 체중변화와 배변상태에 미치는 영향

  • Sin, Hyun-Ju (Department of Food Science and Nutrition, Kyungpook National University) ;
  • Kim, Kwang-Ok (Center for Beautiful Aging, Kyungpook National University) ;
  • Kim, Young-Ah (Department of Food Science and Nutrition, Kyungpook National University) ;
  • Lee, Hye-Sung (Department of Food Science and Nutrition, Kyungpook National University)
  • 신현주 (경북대학교 식품영양학과) ;
  • 김광옥 (경북대학교 장수생활과학연구소) ;
  • 김영아 (경북대학교 식품영양학과) ;
  • 이혜성 (경북대학교 식품영양학과)
  • Received : 2010.02.22
  • Accepted : 2010.09.01
  • Published : 2010.12.31
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Abstract

The present study was conducted to evaluate the effects of resistant starches (RS2, RS3) on weight change and defecation states in rats with constipation induced by loperamide. Sixty male Sprague-Dawley rats were divided into the following six groups: normal control-5% cellulose (NC), constipated-5% cellulose (CC), constipated-5% pectin (CP), constipated-5% RS2 (CR2), constipated-8% RS2 (CR2-H) and constipated-5% RS3 (CR3). The animals were fed AIN76-based experimental diets for 5 weeks. Supplementation of RS2 and RS3 resulted in significantly lower feed efficiency ratios and body weight gain as compared to cellulose supplemented group. Large intestine length was significantly longer in the resistant starch and cellulose groups than in the pectin group. Relative fecal weight to feed intake was significantly higher in the RS3 resistant starch group than in the pectin group, and the appearance of feces was similar to that of the cellulose group. Supplementation of RS3 or cellulose significantly shortened gastrointestinal transit time in the constipated rats as compared to pectin. The results of the present study demonstrated that resistant starch supplementation, especially RS3, may help in depress body weight gain and alleviate constipation through an increase of fecal weight and shortening gastrointestinal transit time in constipated animals.

본 연구는 2종류의 저항전분(RS2, RS3)이 loperamide 유도 변비 쥐의 체중변화와 배변상태에 미치는 영향을 다른 식이섬유 급원과 비교하여 실험 관찰하였다. 실험동물은 Sprague-Dawley계 수컷 쥐 60마리를 사용하였으며 정상 대조군, 변비 셀룰로오스군, 변비 펙틴군, 변비 RS2군, 변비 고RS2군, 변비 RS3군의 6개군으로 나누었다. AIN-76 기본식이에 각각 다른 식이섬유를 첨가하고 에너지 밀도가 동일하게 조제한 실험식을 5주 동안 투여하여 변비 쥐의 식이효율과 체중 변화 및 배변상태와 장통과시간을 분석 비교하여 다음과 같은 결과를 얻었다. 저항전분 투여군들은 셀룰로오스군에 비해서 식이효율과 체중 증가가 유의적으로 낮았고 펙틴군과는 차이가 없었다. 소장의 길이는 모든 식이섬유군간에 유의적 차이가 없었으나, 대장의 길이는 저항전분군들에서 펙틴군에 비해 유의적으로 길었고, 셀룰로오스군과는 유의적인 차이가 없었다. 사료섭취량 대비 분변 배설량은 저항전분 RS2의 경우는 펙틴군 보다는 높았고 셀룰로오스군 보다는 유의적으로 낮았으나 RS3군은 셀룰로오스군과 유의적인 차이가 없이 많았다. 분변의 외형은 RS3군과 고RS2군은 셀룰로오스군과 유사하였으나 저RS2군은 펙틴군과 유사하였다. 변 중 수분함량은 식이섬유 간에 유의적 차이가 없었으나 장 통과시간은 셀룰로오스군과 RS3 투여군이 펙틴군에 비해 유의적으로 단축되는 것으로 나타났다. 본 연구 결과는 변비 동물에서 저항전분의 투여는 식이효율과 체중 증가를 억제하는 작용이 있고, 저항전분중 RS3는 셀룰로오스와 마찬가지로 변비 동물의 배변량을 증가시키고 장 통과시간을 단축시켜 배변 상태를 개선시킬 수 있는 가능성을 나타내었다.

Keywords

References

  1. KCDC. Report on 2007 Korea National Health and Nutrition Examination Survey (KNHANES IV). Korea Centers for Disease Control and Prevention, Seoul, Korea (2007)
  2. Topping DL, Fukushima M, Bird AR. Resistant starch as a prebiotic and synbiotic: State of the art. Proc. Nutr. Soc. 62: 171-176 (2003) https://doi.org/10.1079/PNS2002224
  3. Corfield AP, Carrol D, Myerscouh N, Probert CS. Mucins in the gastrointestinal tract in health and disease. Front. Biosci. 6: 1321-1327 (2001) https://doi.org/10.2741/Corfield
  4. Park JG. Coloproctology. Ilchokak, Seoul, Korea. pp. 273-275 (2005)
  5. Englyst HN, Trowell H, Southgate DAT, Cummings JH. Dietary fiber and resistant starch. Am. J. Clin. Nutr. 46: 873-874 (1987)
  6. Haralampu SG. Resistant starch-a review of physical properties and biological impact of RS3. Carbohydr. Polym. 41: 285-292 (2000) https://doi.org/10.1016/S0144-8617(99)00147-2
  7. Nugent AP. Health properties of resistant starch. Nutr. Bull. 30: 27-54 (2005) https://doi.org/10.1111/j.1467-3010.2005.00481.x
  8. Vanhoof K, De Schrijver R. The influence of enzyme resistant starch on cholesterol metabolism in rats fed on a conventional diet. Brit. J. Nutr. 80: 193-198 (1998)
  9. Lopez HW, Levrat-verny MA, Coudray C, Besson C, Krespine V, Messager A, Demigne C. Rémésy C. Class 2 resistant starches lower plasma and liver lipids and improve mineral retention in rats. J. Nutr. 131: 1283-1289 (2001)
  10. Lopez HW, Coudray C. Bellanger J. Levrat-verny MA, Demigine C, Rayssiguier Y, Remesy C. Resistant starch improves mineral assimilation in rats adapted to a wheat bran diet. Nutr. Res. 20: 141-155 (2000) https://doi.org/10.1016/S0271-5317(99)00146-3
  11. Ferguson LR, Tasman-Jones C, Englyst H, Harris PJ. Comparative effects of three resistant starch preparation on transit time and short chain fatty acid production in rats. Nutr. Cancer 36: 230-237 (2000) https://doi.org/10.1207/S15327914NC3602_13
  12. Seol SM, Bang MH, Choi OS, Kim WK. Effects of high amylose starch on lipid metabolism and immune response in rats. J. Korean Soc. Food. Sci. Nutr. 32: 450-457 (2003) https://doi.org/10.3746/jkfn.2003.32.3.450
  13. Cepinskas G. Adaptive cytoprotection in the small intestine: Role of mucus. Am. J. Physiol. 264: 921-927 (1993)
  14. Shimotoyodome A, Meguro S, Hase T, Tokimitsu I, Sakata T. Decreased colonic mucus in rats with loperamide-induced constipation. Comp. Biochem. Physiol. 126: 203-211 (2000) https://doi.org/10.1016/S1095-6433(00)00194-X
  15. Moundras C, Behr SR, Demigne C, Mazur A, Remesy C. Fermentable polysaccharides that enhance fecal bile acid excretion lower plasma cholesterol and apolipoprotein E-rich HDL in rats. J. Nutr. 124: 2179-2188 (1994)
  16. Schrijver R, Vanhoof KM, Vande GJ. Nutrient utilization in rats and pigs fed enzyme resistant starch. Nutr. Res. 19: 1349-1361 (1999) https://doi.org/10.1016/S0271-5317(99)00092-5
  17. Razadan A, Pettersson D, Pettersson J. Broiler chicken body weights, feed intakes, plasma lipid, and small-intestinal bile acid concentrations in response to feeding of chitosan and pectin. Brit. J. Nutr. 78: 283-291 (1997) https://doi.org/10.1079/BJN19970146
  18. Gordon DT. The importance of total dietary fiber in human nutrition and health. Korean J. Nutr. 25: 75-76 (1992)
  19. Tagliabue A, Raben A, Heijnen ML, Deurenberg P, Pasquali E, Astrup A. The effect of raw potato starch on energy expenditure and substrate oxidation. Am. J. Clin. Nutr. 61: 1070-1075 (1995)
  20. Cummings JH, Beatty ER, Kingman SM, Bingham SA, Englyst HN. Digestion and physiological properties of resistant starch in the human large bowel. Brit. J. Nutr. 75: 733-747 (1996) https://doi.org/10.1079/BJN19960177
  21. Garcia DF, Garcia MV, Bayon JE, Gonzalez GJ. Pectin feeding influences fecal bile acids excretion, hepatic bile acid, and cholesterol synthesis and serum cholesterol in rats. J. Nutr. 126: 1766-1771 (1996)
  22. Wang SG, Yoon EY, Lim YH. Effects of indigestible dextrin on bowel function and serum lipids in rats. J. Korean Soc. Food Sci. Nutr. 25: 560-567 (1996)
  23. Paulini I, Mehta T, Hargis A. Intestinal structural changes in African Green Monkeys after long term psyllium or cellulose feeding. J. Nutr. 117: 253-266 (1987)
  24. Johnson IT, Gee JM, Mahoney RR. Effect of dietary supplements of guar gum and cellulose on intestinal cell proliferation, enzyme levels, and sugar transport in the rat. Brit. J. Nutr. 52: 477-487 (1984) https://doi.org/10.1079/BJN19840115
  25. Topping DL, Gooden JM, Brown IL, Biebrick DA, McGrath L, Trimble RP, Choct M, Illman RJ. A high amylose (amylomaize) starch raises proximal large bowel starch and increases colon length in pig. J. Nutr. 127: 615-622 (1997)
  26. Kim MK, Lee SS. The effect of dietary fiber on the serum lipid level and bowel function in rats. Korean J. Nutr. 28: 23-32 (1995)
  27. Dowling RH. The intestinal response to high bulk feeding in the rat. Clin. Sci. 32: 1-9 (1967)
  28. Park SH, Lee YK, Lee HS. The effect of dietary fiber feeding on gastrointestinal functions and lipid and glucose metabolism in streptozotocin-induced diabetic rats. Korean J. Nutr. 27: 311-322 (1994)
  29. Cummings JH, Bingham SA, Heaton KW, Eastwood MA. Fecal weight, colon cancer risk and dietary intake of nonstarch polysaccharides (dietary fiber). Gastroenterology 103: 1783-1789 (1992)
  30. Brisinda G, Cadeddu F, Maria G, Laughton J, Lembo A, Camilleri M. Chronic constipation. New Engl. J. Med. 350: 1259-1260 (2004) https://doi.org/10.1056/NEJM200403183501219
  31. Corinne M, Stephen RB, Christian D, Andrzej M, Christian R. Fermentable polysaccharides that enhance fecal bile acid excretion lower plasma cholesterol and apolipoprotein E-rich HDL in rats. J. Nutr. 124: 2179-2188 (1994)
  32. Heijen MLA, Van Amelsvoort JMM, Deurenberg P, Beynen AC. Limited effect of consumption of uncooked (RS2) or retrograded (RS3) resistant starch on putative risk factors for colon cancer in healthy men. Am. J. Clin. Nutr. 67: 322-331 (1998)
  33. Gordon DT. Total dietary fiber and mineral absorption. Korean J. Nutr. 25: 429-449 (1992)
  34. Spiller GA, Chernoff MC, Hill RA, Gates JE, Nassar JJ, Shipley EA. Effect of purified cellulose, pectin, and a low residue diet on fecal volatile fatty acids, transit time and fecal weight in humans. Am. J. Clin. Nutr. 33: 754-759 (1980)
  35. Murakami K, Sasaki S, Okubo H, Takahashi Y, Hosoi Y, Itabashi M. Association between dietary fiber, water and magnesium intake and functional constipation among young Japanese women. Eur. J. Clin. Nutr. 61: 616-622 (2007)
  36. Tsuji K. Nutritional role of dietary fiber-recent knowledge on dietary fiber. Korean J. Food Hygiene 7: S73-S76 (1992)
  37. Hillman L, Peters S, Fisher A, Pomare EW. Differing effects of pectin, cellulose and lignin on stool pH, transit time, and weight. Br. J. Nutr. 50: 189-195 (1983) https://doi.org/10.1079/BJN19830088
  38. Kim JY, Kim WK, Kim HY, Kim MH, Kim SH. Effects of soy oligosaccharides and varing dietary fats on intestinal microflora, lipid profiles, and immune responses in rats. Korean J. Nutr. 33: 597-612 (2000)
  39. Blackburn NA, Johnson IT. The effect of guar gum on the viscosity of the gastrointestinal contents and glucose uptake form the perfused jejunum in the rat. Brit. J. Nutr. 46: 239-246 (1981) https://doi.org/10.1079/BJN19810029