Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Isolation of a Calcium-Binding Fraction from a Hot-Water Extract of Smilax rhizoma

청미래덩굴 뿌리 열수 추출물로부터 칼슘 결합 물질의 분리

  • Lee, Ji-Hye (Department of Food Science & Technology, Chungnam National University) ;
  • Jeon, So-Jeong (Department of Food Science & Technology, Chungnam National University) ;
  • Song, Kyung-Bin (Department of Food Science & Technology, Chungnam National University)
  • 이지혜 (충남대학교 농업생명과학대학 식품공학과) ;
  • 전소정 (충남대학교 농업생명과학대학 식품공학과) ;
  • 송경빈 (충남대학교 농업생명과학대학 식품공학과)
  • Received : 2010.07.22
  • Accepted : 2010.12.03
  • Published : 2010.12.30
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Abstract

We isolated a calcium-binding substance from Smilacis rhizoma hot-water extract using ion exchange, normal phase HPLC, and gel filtration chromatography; fractions were analyzed for calcium-binding activity. Fractions (F6) with the highest calcium-binding activity from the resource Q coulmn were pooled and further purified on an $NH_2$ column. Two major peaks were separated and the fraction (F61) with the higher calcium-binding activity was then loaded onto a $Superdex^{TM}$ column. A single peak (F611) with calcium-binding activity was finally obtained. These results suggest that the isolated calcium-binding fraction could be used as a functional food additive, similar to a calcium supplement, in the food industry.

청미래덩굴 뿌리(Smilacis rhizoma)로부터 칼슘과 결합하는 물질을 분리하고자 열수로 추출한 추출물을 ion exchange, normal-phase HPLC 및 gel filtration chromatogarphy를 이용하여 칼슘 결합 물질을 순차적으로 분리하였다. 그 결과 ion exchange chromatography에서 7개의 major peaks를 얻었으며, 이 중 F6 fraction이 0.083 mM로 칼슘과 가장 높은 결합력을 가졌다. 또한 F6를 $NH_2$ column으로 분획한 결과 F61에서 0.130 mM의 가장 높은 칼슘함량을 나타내었으며, 최종적으로 $Superdex^{TM}$를 이용하여 F611 fraction으로 분리하였다. 따라서 청미래덩굴 뿌리 추출물 중 F611 fraction을 이용하여 biomineral을 제조함으로써 칼슘 보충제나 기능성 성분의 원료로써 식품산업에 활용될 수 있다고 판단된다.

Keywords

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